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Integration of white shrimp (Litopenaeus vannamei) and green seaweed (Ulva prolifera) in minimum-water exchange aquaculture system

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Abstract

Ulva prolifera is one of the most common macroalgae and is distributed widely off the coast of China. It is well known for its rapid growth rate and good reproduction and it has fast nitrogen removal efficiency. Integration of seaweed cultivation in shrimp farming water is considered a potential aquaculture practice since seaweeds can convert dissolved inorganic nitrogen into biomass and the biomass can be harvested easily. This study investigated the effect of integrating green seaweed (Ulva prolifera) with Litopenaeus vannamei (500 shrimp m−3) at varying levels of water exchange daily on water quality and shrimp growth performance. The four daily water exchange quantities were 5% (T1), 10% (T2), 15% (T3), and 20% (T4). The appropriate range of the stocking density of U. prolifera was 800 mg L−1. No significant differences were observed in total ammonia nitrogen (TAN) concentration between T2 and T3 (P > 0.05) from beginning to end. The concentrations of nitrite and nitrate in all treatment remained constant at low levels from beginning to the end. On day 35, there were no significant differences in survival rate of shrimp among T2, T3, and T4. No significant differences in FCR were observed in group T2, as compared to T1, T3, or T4. No significant differences in cumulative weight of U. prolifera were observed among T1, T2, and T3; however, they were significantly larger than that of T4. The study demonstrates that integrating U. prolifera (800 mg L−1) with L. vannamei (500 shrimp m−3) with 10% water exchange can control the water quality and enhance shrimp growth.

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References

  • Akbary P, Aminikhoei Z (2018) Effect of water-soluble polysaccharide extract from the green alga Ulva rigida on growth performance, antioxidant enzyme activity, and immune stimulation of grey mullet Mugil cephalus. J Appl Phycol 30:1345–1353

    Article  CAS  Google Scholar 

  • Azim ME, Little DC (2008) The biofloc technology (BFT) in indoor tanks: water quality, biofloc composition, and growth and welfare of Nile tilapia (Oreochromis niloticus). Aquaculture 283:29–35

    Article  CAS  Google Scholar 

  • Baloo L, Azman S, Said MIM, Ahmad F, Mohamad M (2014) Biofiltration potential of macroalgae for ammonium removal in outdoor tank shrimp wastewater recirculation system. Biomass Bioenergy 66:103–109

    Article  CAS  Google Scholar 

  • Berri M, Slugocki C, Olivier M, Helloin E, Jacques I, Salmon H, Demais H, Le Goff M, Collen PN (2016) Marine-sulfated polysaccharides extract of Ulva armoricana green algae exhibits an antimicrobial activity and stimulates cytokine expression by intestinal epithelial cells. J Appl Phycol 28:2999–3008

    Article  Google Scholar 

  • Bikker P, van Krimpen MM, van Wikselaar P, Houweling-Tan B, Scaccia N, van Hal JW, Huijgen WJJ, Cone JW, López-Contreras AM (2016) Biorefinery of the green seaweed Ulva lactuca to produce animal feed, chemicals and biofuels. J Appl Phycol 28:3511–3525

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Bray WA, Williams RR, Lightner DV, Lawrence AL (2006) Growth, survival and histological responses of the marine shrimp, Litopenaeus vannamei, to three dosage levels of oxytetracycline. Aquaculture 258:97–108

    Article  CAS  Google Scholar 

  • Brito LO, Arantes R, Magnotti C, Derner R, Pchara F, Olivera A (2014) Water quality and growth of Pacific white shrimp Litopenaeus vannamei (Boone) in co-culture with green seaweed Ulva lactuca (Linnaeus) in intensive system. Aquacult Int 22:497–508

    Article  CAS  Google Scholar 

  • Cahill PL, Hurd CL, Lokman M (2010) Keeping the water clean — seaweed biofiltration outperforms traditional bacterial biofilms in recirculating aquaculture. Aquaculture 306:153–159

    Article  Google Scholar 

  • Castro R, Zarra I, Lamas J (2004) Water-soluble seaweed extracts modulate the respiratory burst activity of turbot phagocytes. Aquaculture 229:67–78

    Article  Google Scholar 

  • Copertino MDS, Tormena T, Seeliger U (2009) Biofiltering efficiency, uptake and assimilation rates of Ulva clathrata (Roth) J. Agardh (Chlorophyceae) cultivated in shrimp aquaculture waste water. J Appl Phycol 21:31–45

    Article  CAS  Google Scholar 

  • Cuzon G, Lawrence A, Gaxiola G, Rosas C, Guillaume J (2004) Nutrition of Litopenaeus vannamei reared in tanks or in ponds. Aquaculture 235:513–551

    Article  CAS  Google Scholar 

  • Cruz-Suárez LE, León A, Peňa-Rodríguez A, Rodríguez-Peňa G, Moll B, Ricque-Marie D (2010) Shrimp/Ulva co-culture: a sustainable alternative to diminish the need for artificial feed and improve shrimp quality. Aquaculture 301:64–68

    Article  Google Scholar 

  • Esparza-Leal HM, Xavier JAA, Jr WW (2016) Performance of Litopenaeus vannamei, postlarvae reared in indoor nursery tanks under biofloc conditions at different salinities and zero-water exchange. Aquacult Int 24:1–13

    Article  CAS  Google Scholar 

  • Furtado PS, Campos BR, Serra FP, Klosterhoff M, Romano LA, Wasielesky W Jr (2015) Effects of nitrate toxicity in the Pacific white shrimp, Litopenaeus vannamei, reared with biofloc technology (BFT). Aquacult Int 23:315–327

    Article  CAS  Google Scholar 

  • Gao G, Zhong ZH, Zhou XH, Xu JT (2016) Changes in morphological plasticity of Ulva prolifera under different environmental conditions: a laboratory experiment. Harmful Algae 59:51–58

    Article  PubMed  Google Scholar 

  • Ge HX, Li J, Chang ZQ, Chen P, Shen MM, Zhao FZ (2016a) Effect of microalgae with semicontinuous harvesting on water quality and zootechnical performance of white shrimp reared in the zero water exchange system. Aquacult Eng 72-73:70–76

    Article  Google Scholar 

  • Ge HX, Li J, Chen P, Chang ZQ, Shen MM, Zhao FZ (2016b) Cultivation of Platymonas helgolandica in rearing water enhances the growth performance and resistance of Litopenaeus vannamei against Vibrio parahaemolyticus infection. Aquacult Int 25:1279–1290

    Article  CAS  Google Scholar 

  • Guerdat TC, Losordo TM, Classen JJ, Osborne JA, DeLong DP (2010) An evaluation of commercially available biological filters for recirculating aquaculture systems. Aquacult Eng 42:38–49

    Article  Google Scholar 

  • Han JE, Mohney LL, Tang KFJ, Pantoja CR, Lightner DV (2015) Plasmid mediated tetracycline resistance of Vibrio parahaemolyticus associated with acute hepatopancreatic necrosis disease (AHPND) in shrimps. Aquacult Rep 2:17–21

    Article  Google Scholar 

  • Holl CM, Glazer CT, Moss SM (2011) Nitrogen stable isotopes in recirculating aquaculture for super-intensive shrimp production: tracing the effects of water filtration on microbial nitrogen cycling. Aquaculture 311:146–154

    Article  CAS  Google Scholar 

  • Jiao L, Li X, Li T, Jiang P, Zhang L, Wu M (2009) Characterization and anti-tumor activity of alkali-extracted polysaccharide from Enteromorpha intestinalis. Int Immunopharmacol 9:324–329

    Article  CAS  PubMed  Google Scholar 

  • Khoi LV, Fotedar R (2011) Integration of western king prawn (Penaeus latisulcatus Kishinouye, 1896) and green seaweed (Ulva lactuca Linnaeus, 1753) in a closed recirculating aquaculture system. Aquaculture 322-323:201–209

    Article  Google Scholar 

  • Kim JK, Cho ML, Karnjanapratum S, Shin IS, You SG (2011) In vitro and in vivo immunomodulatory activity of sulfated polysaccharides from Enteromorpha prolifera. Int J Biol Macromol 49:1051–1058

    Article  CAS  PubMed  Google Scholar 

  • Kuhn DD, Drahos DD, Marsh L, Flick GJ Jr (2010a) Evaluation of nitrifying bacteria product to improve nitrification efficacy in recirculating aquaculture systems. Aquacult Eng 43:78–82

    Article  Google Scholar 

  • Kuhn DD, Smith SA, Boardman GD, Angier MW, Marsh L, Flick GJ Jr (2010b) Chronic toxicity of nitrate to Pacific white shrimp, Litopenaeus vannamei: impacts on survival, growth, antennae length, and pathology. Aquaculture 309:109–114

    Article  CAS  Google Scholar 

  • Li H, Zhang Y, Chen J, Zheng X, Liu F, Jiao N (2018a) Nitrogen uptake and assimilation preferences of the main green tide alga Ulva prolifera in the Yellow Sea, China. J Appl Phycol. https://doi.org/10.1007/s10811-018-1575-2

  • Li Y, Huang Z, Qiao L, Gao Y, Guan H, Hwan H (2015) Purification and characterization of a novel enzyme produced by Catenovulum sp. LP and its application in the pre-treatment to Ulva prolifera for bio-ethanol production. Process Biochem 50:799–806

    Article  CAS  Google Scholar 

  • Li W, Wang K, Jiang N, Liu X, Wan M, Chang X, Liu D, Qi H, Liu S (2018b) Antioxidant and antihyperlipidemic activities of purified polysaccharides from Ulva pertusa. J Appl Phycol 30:2619–2627

    Article  CAS  Google Scholar 

  • Liu D, Keesing JK, Xing Q, Shi P (2009) World's largest macroalgal bloom caused by expansion of seaweed aquaculture in China. Mar Poll Bull 58:888–895

    Article  CAS  Google Scholar 

  • Mantri VA, Singh RP, Bijo AJ, Kumari P, Reddy CRK, Jha B (2011) Differential response of varying salinity and temperature on zoospore induction, regeneration and daily growth rate in Ulva fasciata (Chlorophyta, Ulvales). J Appl Phycol 23:243–250

    Article  Google Scholar 

  • Murphy C, Hotchkiss S, Worthington J, McKeown SR (2014) The potential of seaweed as a source of drugs for use in cancer chemotherapy. J Appl Phycol 26:2211–2264

    Article  CAS  Google Scholar 

  • Neori A, Chopin T, Troell M, Buschmann AH, Kraemer GP, Halling C, Shpigel M, Yarish C (2004) Integrated aquaculture: rationale, evolution and state of the art emphasizing seaweed biofiltration in modern mariculture. Aquaculture 231:361–391

    Article  Google Scholar 

  • Pintar A, Besson M, Gallezot P, Gibert J, Martin D (2004) Toxicity to Daphnia magna and Vibrio fischeri of Kraft bleach plant effluents treated by catalytic wet-air oxidation. Water Res 38:289–300

    Article  CAS  PubMed  Google Scholar 

  • Pungrasmi W, Phinitthanaphak P, Powtongsook S (2016) Nitrogen removal from a recirculating aquaculture system using a pumice bottom substrate nitrification-denitrification tank. Ecol Eng 95:357–363

    Article  Google Scholar 

  • Qiu X, Neori A, Kim JK, Yarish C, Shpigel M, Guttman L, Ben Ezra D, Odintsov V, Davis DA (2018) Evaluation of green seaweed Ulva sp. as a replacement of fish meal in plant-based practical diets for Pacific white shrimp, Litopenaeus vannamei. J Appl Phycol 30:1305–1316

    Article  CAS  Google Scholar 

  • Samocha TM, Fricher J, Ali AM, Shpigel M, Neori A (2015) Growth and nutrient uptake of the macroalga Gracilaria tikvahiae cultured with the shrimp Litopenaeus vannamei in an integrated multi-trophic aquaculture (IMTA) system. Aquaculture 446:263–271

    Article  Google Scholar 

  • Shao LL, Xu J, Shi MJ, Wang XL, Li YT, Kong LM, Hider RC, Zhao T (2017) Preparation, antioxidant and antimicrobial evaluation of hydroxamated degraded polysaccharides from Enteromorpha prolifera. Food Chem 237:481–487

    Article  CAS  PubMed  Google Scholar 

  • Shi MJ, Wei XY, Xu J, Chen BJ, Zhao DY, Cui S, Zhou T (2017) Carboxymethylated degraded polysaccharides from Enteromorpha prolifera: preparation and in vitro antioxidant activity. Food Chem 215:76–83

    Article  CAS  PubMed  Google Scholar 

  • Sun KM, Li RX, Li YM, Xiao J, Wang ZL, Tang XX, Pang M (2015) Responses of Ulva prolifera to short-term nutrient enrichment under light and dark conditions. Estuar Coast Shelf Sci 163:56–62

    Article  CAS  Google Scholar 

  • Teng ZL, Qian L, Zhou Y (2013) Hypolipidemic activity of the polysaccharides from Enteromorpha prolifera. Int J Biol Macromol 62:254–256

    Article  CAS  PubMed  Google Scholar 

  • van Rijn J (2013) Waste treatment in recirculating aquaculture systems. Aquac Eng 53:49–56

    Article  Google Scholar 

  • Vinatea L, Gálvez AO, Browdy CL, Stokes A, Venero J, Haveman J, Lewis BL, Lawson A, Shuler A, Leffler JW (2010) Photosynthesis, water respiration and growth performance of Litopenaeus vannamei in a super-intensive raceway culture with zero water exchange: interaction of water quality variables. Aquacult Eng 42:17–24

    Article  Google Scholar 

  • Wang H, Liu CF, Qin CX (2007a) Using a macroalgae Ulva pertusa biofilter in a recirculating system for production of juvenile sea cucumber Apostichopus japonicus. Aquacult Eng 36:217–224

    Article  Google Scholar 

  • Wang H, Liu CF, Qin CX, Cao SQ, Ding J (2007b) Using a macroalgae Ulva pertusa biofilter in a recirculating system for production of juvenile sea cucumber Apostichopus japonicas. Aquacult Eng 36:217–224

    Article  Google Scholar 

  • Wang P, Wu CW, Zhou ZZ (2010) Ecological effects of Ulva lactuca on Sebastiscus marmoratus aquculture. South China Fish Sci 6:63–67

    CAS  Google Scholar 

  • Wik TEI, Lindén BT, Wramner PI (2009) Integrated dynamic aquaculture and wastewater treatment modelling for recirculating aquaculture systems. Aquaculture 287:361–370

    Article  CAS  Google Scholar 

  • Xu WJ, Pan LQ (2012) Effects of bioflocs on growth performance, digestive enzyme activity and body composition of juvenile Litopenaeus vannamei in zero-water exchange tanks manipulating C/N ratio in feed. Aquaculture 356-357:147–152

    Article  CAS  Google Scholar 

  • Yabe T, Ishii Y, Amano Y, Koga T, Hayashi S, Nohara S, Tatsumoto H (2009) Green tide formed by free-floating Ulva spp. at Yatsu tidal flat. Japan Limnol 10:239–245

    Article  Google Scholar 

  • Zhang Z, Wang X, Yu S (2011) Synthesized oversulfated and acetylated derivatives of polysaccharide extracted from Enteromorpha linza and their potential antioxidant activity. Int J Biol Macromol 49:1012–1015

    Article  CAS  PubMed  Google Scholar 

  • Zhao J, Jiang P, Liu ZY, Wang JF, Cui YL, Qin S (2011) Genetic variation of Ulva (Enteromorpha) prolifera (Ulvales, Chlorophyta) – the causative species of the green tides in the Yellow Sea. China J Appl Phycol 23:227–233

    Article  Google Scholar 

  • Zhu SM, Deng YL, Ruan YJ, Guo XS, Shi MM, Shen JZ (2015) Biological denitrification using poly (butylene succinate) as carbon source and biofilm carrier for recirculating aquaculture system effluent treatment. Bioresour Technol 192:603–610

    Article  CAS  PubMed  Google Scholar 

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Acknowledgements

The authors are grateful to all the laboratory members for experimental material preparation and technical assistance.

Funding

This study was supported by the earmarked fund for Modern Agro-industry Technology Research System (No.CARS-48), the Program of Shandong Leading Talent (No.LNJY2015002), Su bei science and technology special program (No.SZ-LYG2017029), the Huaihai Institute of Technology start-up funds (No. KQ17022), Open-end Funds of Jiangsu Key Laboratory of Marine Biotechnology (No. HS2017002) and the Priority Academic Program Development of Jiangsu Higher Education Institutions.

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Authors and Affiliations

  1. Jiangsu Key Laboratory of Marine Bioresources and Environment, Huaihai Institute of Technology, Lianyungang, 222005, Jiangsu Province, China

    Hong-xing Ge

  2. Key Laboratory for Sustainable Development of Marine Fisheries, Ministry of Agriculture; Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, 266071, Shandong Province, China

    Hong-xing Ge, Jian Li, Ji-tao Li, Zhao Chen & Fa-zhen Zhao

  3. Jiangsu Key Laboratory of Marine Biotechnolog, Huaihai Institute of Technology, Lianyungang, 222005, Jiangsu Province, China

    Hong-xing Ge & Qian Ni

  4. Co-Innovation Center of Jiangsu Marine Bio-industry Technology, Huaihai Institute of Technology, Lianyungang, 222005, Jiangsu Province, China

    Hong-xing Ge

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  1. Hong-xing Ge
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  2. Qian Ni
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  3. Jian Li
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  4. Ji-tao Li
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Correspondence to Jian Li.

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Ge, Hx., Ni, Q., Li, J. et al. Integration of white shrimp (Litopenaeus vannamei) and green seaweed (Ulva prolifera) in minimum-water exchange aquaculture system. J Appl Phycol 31, 1425–1432 (2019). https://doi.org/10.1007/s10811-018-1601-4

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