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Hyperbaric Oxygen Alleviates the Inflammatory Response Induced by LPS Through Inhibition of NF-κB/MAPKs-CCL2/CXCL1 Signaling Pathway in Cultured Astrocytes

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Abstract

The purpose of this study was to investigate the inhibition neuroinflammation mechanisms of hyperbaric oxygen therapy (HBOT). Primary astrocytes were incubated with lipopolysaccharide (LPS) after which they underwent HBOT and separate administration of inflammatory cytokine inhibitors. The respective expression of inflammatory factors was then detected. Results showed that LPS significantly induced increases in the expression levels of chemokine (C-X-C motif) ligand 1 (CXCL1), chemokine C-C motif ligand 2 (CCL2), phospho-nuclear factor-kappa B (p-NF-κB), phospho-c-Jun N-terminal kinase (p-JNK), phospho-extracellular signal-regulated kinase (p-ERK), and phospho-p38 (p-p38) in cultured astrocytes and peaked at 3 h. HBOT downregulated the expression of some inflammation mediators including CXCL1 and CCL2. Furthermore, HBOT inhibited the expression of some up-stream regulators of inflammation mediators including p-NF-κB, p-JNK, p-p38 (at 3 and 6 h), and p-ERK (3 h). Inhibitors of NF-κB, ERK, and JNK (BAY117082, PD98059, and SP600125) significantly suppressed the expression of CXCL1 and CCL2 that were induced by LPS for 3 h. However, the p38 inhibitor, SB203580, had no obvious effect on expression levels of CXCL1 and CCL2. In conclusion, we found that HBOT inhibits neuroinflammation via regulation of the LPS-induced NF-κB/mitogen-activated protein kinases (MAPKs, JNK, and ERK) -CCL2/CXCL1 signaling pathways.

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Funding

This work was funded by the National Natural Science Foundation of China (NSFC 81702223) and the Science and Technology Planning Project of Nantong (MS22016044).

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Author notes

  1. Su Liu and Chun Lu contributed equally to this work.

Authors and Affiliations

  1. Department of Rehabilitation Medicine, Affiliated Hospital of Nantong University, Nantong, China

    Su Liu, Xiaoyun Zhou, Li Sun, Qi Gu, Guangyu Shen & Aisong Guo

  2. School of Medicine, Nantong University, Nantong, China

    Chun Lu

  3. Department of Pathology, Affiliated Hospital of Nantong University, Nantong, China

    Ying Liu

Authors
  1. Su Liu
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  2. Chun Lu
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  3. Ying Liu
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  4. Xiaoyun Zhou
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  5. Li Sun
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  6. Qi Gu
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Correspondence to Aisong Guo.

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Liu, S., Lu, C., Liu, Y. et al. Hyperbaric Oxygen Alleviates the Inflammatory Response Induced by LPS Through Inhibition of NF-κB/MAPKs-CCL2/CXCL1 Signaling Pathway in Cultured Astrocytes. Inflammation 41, 2003–2011 (2018). https://doi.org/10.1007/s10753-018-0843-2

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  • DOI: https://doi.org/10.1007/s10753-018-0843-2

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