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Expression of CXCR1 (Interleukin-8 Receptor) in Murine Macrophages After Staphylococcus aureus Infection and its Possible Implication on Intracellular Survival Correlating with Cytokines and Bacterial Anti-Oxidant Enzymes

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Abstract

Interaction with the live Staphylococcus aureus promotes secretion of interleukin-8 (IL-8), although the expressions of functional CXCR1 (IL-8RA) in murine macrophages have not been identified. Expression of CXCR1 was induced in S. aureus-infected macrophages, whereas, CXCR1 was undetectable in control macrophages. CXCR1 blocking significantly reduced the phagocytosis of S. aureus and TNF-α, IL-6, IL-1β, IFN-γ, IL-12, and IL-8 production and increased release of MIP-2 and soluble TNF-R1. Increased bacterial catalase and decreased superoxide dismutase (SOD) activities by S. aureus with concomitant decrease in hydrogen peroxide (H2O2), superoxide anion, and nitric oxide (NO) release were observed in case of prior CXCR1 blocking. In the presence of cytochalasin D, S. aureus-mediated induction of IL-8 was inhibited concomitant with decreased bacterial count suggesting that internalization of S. aureus was necessary for induction of IL-8. Shedding of TNF-R1 due to CXCR1 blocking after S. aureus inoculation was critical for neutralization of TNF-α signaling and arrests the inflammation.

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Abbreviations

CPCSEA:

Committee for the Purpose of Control and Supervision of Experiments on Animal

CXC-chemokine:

Having a single amino acid residue interposed between the first two canonical cysteines

CXCL-8:

Interleukin-8

CXCR1-CXC:

Chemokine receptor-1

EDTA:

Ethylenediaminetetraacetic acid

ELR+ :

Glutamate–leucine–arginine (ELR) motif near the N terminal

FBS:

Fetal bovine serum

HBSS:

Hank’s balanced salt solution

IL-8RA:

Interleukin-8 receptor A

iNOS:

Inducible nitric oxide synthase

JNK:

C jun N-terminal kinase

MAPK:

Mitogen-activated protein kinase

MHC-II:

Major histocompatibility complex II

NaNo3 :

Sodium nitrate

NaOH:

Sodium hydroxide

NF-κB:

Nuclear transcription factor kappa beta

NOS2:

Nitric oxide synthase-2

PBMC:

Peripheral blood mononuclear cells

PIP3:

Phospho inositol trisphosphate

PMSF:

Phenyl methyl sulfonyl fluoride

RIPA:

Radio immune precipitation assay buffer

SDS:

Sodium dodecyl sulfate

sTNF-R1:

Soluble TNF receptor type1

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Acknowledgments

This work was supported by the Department of Science and Technology (DST), Science and Engineering Research Board (SERB), Ministry of Science and Technology, Government of India, New Delhi, India [grant number SR/SO/HS/0013/2012, dated 21 May 2013 to BB] for funding this project. The author (Biswadev Bishayi) is indebted to the Department of Science and Technology, Government of India for providing us with the instruments procured under the DST-PURSE program to the Department of Physiology, University of Calcutta.

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  1. Department of Physiology, Immunology Laboratory, University of Calcutta, University Colleges of Science and Technology, 92 APC Road, Calcutta, 700009, West Bengal, India

    Biswadev Bishayi, Arnab Majhi & Rana Adhikary

  2. Department of Physiology, Oxidative Stress and Free Radical Biology Laboratory, University of Calcutta, University Colleges of Science and Technology, 92 APC Road, Calcutta, 700009, West Bengal, India

    Debasish Bandyopadhyay

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Bishayi, B., Bandyopadhyay, D., Majhi, A. et al. Expression of CXCR1 (Interleukin-8 Receptor) in Murine Macrophages After Staphylococcus aureus Infection and its Possible Implication on Intracellular Survival Correlating with Cytokines and Bacterial Anti-Oxidant Enzymes. Inflammation 38, 812–827 (2015). https://doi.org/10.1007/s10753-014-9991-1

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