Abstract
Our earlier studies proposed a radically new idea suggesting interdependency between TNF-α/TNFR1 and IL-1β/IL-1R pathways in modulation of Staphylococcus aureus-induced CXCL8/CXCR1 axis. However, the effects of inhibition of cytokine receptor mobilization at intracellular level and surface TNFR1 and IL-1R shedding on S. aureus-induced CXCR1 expression have not been studied so far in peritoneal macrophages. This study aimed to investigate the role of inhibition of receptor mobilization from the intracellular pool (using brefeldin A) and surface receptor shedding (using TAPI-1) on CXCR1 expression during dual receptor (TNFR1 plus IL-1R) neutralization in peritoneal macrophages isolated from wild-type Swiss Albino mice. Release of superoxide anion, nitric oxide, and hydrogen peroxide was measured and cytokine production was done by ELISA. Expression of surface receptors (TNFR1, IL-1R, and CXCR1) and inflammatory mediators was studied by Western blot. It was observed that S. aureus-infected macrophages showed elevated ROS production, secretion of TNF-α, IL-1β, and CXCL8, along with increased expression of surface receptors (TNFR1, IL-1R, and CXCR1), and inflammatory markers (iNOS and COX-2) compared with control or treated groups (p < 0.05). However, prior treatment of macrophages with BFA or TAPI-1 in the presence of anti-TNFR1 antibody and IRAP during S. aureus infection showed significant reduction of all these parameters (p < 0.05). We can conclude that targeting of TNFR1 and IL-1R (with major focus on surface expression study) either through blockage of intracellular receptor trafficking pathway or via surface receptor shedding diminishes TNFR1/IL-1R interaction and consequently downregulates CXCR1 expression along with inflammatory signalling pathways during bacterial infections.
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References
Cassado Ados A, D’Império Lima MR, Bortoluci KR. Revisiting mouse peritoneal macrophages: heterogeneity, development, and function. Front Immunol. 2015;6:225.
Gordon RJ, Lowry FD. Pathogenesis of methicillin-resistant Staphylococcus aureus infection. Clin Infect Dis. 2008;46:350–9.
Parameswaran N, Patial S. Tumor necrosis factor-α signaling in macrophages. Crit Rev Eukaryot Gene Expr. 2010;20:87–103.
Yimin, Kohanawa M, Zhao S, Ozaki M, Haga S, Nan G, et al. Contribution of toll-like receptor 2 to the innate response against Staphylococcus aureus infection in mice. PLoS One. 2013;8(9):e74287. https://doi.org/10.1371/journal.pone.0074287.
Fournier B. The function of TLR2 during staphylococcal diseases. Front Cell Infect Microbiol. 2013;2:1–8.
Matsushima K, Morishita K, Yoshimura T, Lavu S, Kobayashi Y, Lew W, et al. Molecular cloning of a human monocyte-derived neutrophil chemotactic factor (MDNCF) and the induction of MDNCF mRNA by interleukin 1 and tumor necrosis factor. J Exp Med. 1988;167:1883–93.
Hall DA, Beresford IJ, Browning C, Giles H. Signalling by CXC-chemokine receptors 1 and 2 expressed in CHO cells: a comparison of calcium mobilization, inhibition of adenylyl cyclase and stimulation of GTPgammaS binding induced by IL-8 and GRO alpha. Br J Pharmacol. 1999;126:810–8.
Fan X, Patera AC, Pong-Kennedy A, Deno G, Gonsiorek W, Manfra DJ, et al. Murine CXCR1 is a functional receptor for GCP-2/CXCL6 and interleukin-8/CXCL8. J Biol Chem. 2007;282:11658–66.
Bishayi B, Nandi A, Dey R, Adhikary R. Expression of CXCR1 (IL-8 receptor A) in splenic, peritoneal macrophages and resident bone marrow cells after acute live or heat killed Staphylococcus aureus stimulation in mice. Microb Pathog. 2017;109:131–50.
Bishayi B, Adhikary R, Sultana S, Dey R, Nandi A. Altered expression of CXCR1 (IL-8R) in macrophages utilizing cell surface TNFR1 and IL-1 receptor during Staphylococcus aureus infection. Microb Pathog. 2017;113:460–71.
McCann FE, Perocheau DP, Ruspi G, Blazek K, Davies ML, Feldmann M, et al. Selective tumor necrosis factor receptor I blockade is antiinflammatory and reveals immunoregulatory role of tumor necrosis factor receptor II in collagen-induced arthritis. Arthritis Rheum. 2014;66:2728–38.
Marriott HM, Gascoyne KA, Gowda R, Geary I, Nicklin MJ, Iannelli F, et al. Interleukin-1β regulates CXCL8 release and influences disease outcome in response to Streptococcus pneumoniae, defining intercellular cooperation between pulmonary epithelial cells and macrophages. Infect Immun. 2012;80:1140–9.
Boraschi D, Tagliabue A. The interleukin-1 receptor family. Semin Immunol. 2013;25:394–407.
Jayaraman P, Sada-Ovalle I, Nishimura T, Anderson AC, Kuchroo VK, Remold HG, et al. IL-1β promotes antimicrobial immunity in macrophages by regulating TNFR signaling and caspase-3 activation. J Immunol. 2013;190:4196–204.
Olaru F, Jensen LE. Staphylococcus aureus stimulates neutrophil targeting chemokine expression in keratinocytes through an autocrine IL-1alpha signalling loop. J Invest Dermatol. 2010;130:1866–76.
Okamoto S, Mukaida N, Yasumoto K, Horiguchi H, Matsushima K. Molecular mechanism of interleukin-8 gene expression. Adv Exp Med Biol. 1993;351:87–97.
Roebuck KA. Regulation of interleukin-8 gene expression. J Interf Cytokine Res. 1999;19:429–38.
Wolf JS, Chen Z, Dong G, Sunwoo JB, Bancroft CC, Capo DE, et al. IL (interleukin)-1alpha promotes nuclear factor-kappaB and AP-1-induced IL-8 expression, cell survival, and proliferation in head and neck squamous cell carcinomas. Clin Cancer Res. 2001;7:1812–20.
Fujiwara T, Oda K, Yokota S, Takatsuki A, Ikehara Y. Brefeldin A causes disassembly of the Golgi complex and accumulation of secretory proteins in the endoplasmic reticulum. J Biol Chem. 1988;263:18545–52.
Ripley CR, Fant J, Bienkowski RS. Brefeldin A inhibits degradation as well as production and secretion of collagen in human lung fibroblasts. J Biol Chem. 1993;268:3677–82.
Bao S, Smith RM, Jarett L, Garvey WT. The effects of brefeldin A on the glucose transport system in rat adipocytes. Implications regarding the intracellular locus of insulin-sensitive Glut4. J Biol Chem. 1995;270:30199–204.
Wyrozumska P, Ashley JW, Ramanadham S, Liu Q, Garvey WT, Sztul E. Novel effects of brefeldin A (BFA) in signaling through the insulin receptor (IR) pathway and regulating FoxO1-mediated transcription. Cell Logist. 2014;4:e27732.
Storey H, Stewart A, Vandenabeele P, Luzio JP. The p55 tumour necrosis factor receptor TNFR1 contains a trans-Golgi network localization signal in the C-terminal region of its cytoplasmic tail. Biochem J. 2002;366:15–22.
Reddy P, Slack JL, Davis R, Cerretti DP, Kozlosky CJ, Blanton RA, et al. Functional analysis of the domain structure of tumor necrosis factor-alpha converting enzyme. J Biol Chem. 2000;275:14608–14.
Fischer R, Kontermann RE, Maier O. Targeting sTNF/TNFR1 signaling as a new therapeutic strategy. Antibodies. 2015;4:48–70.
Croft M, Benedict CA, Ware CF. Clinical targeting of the TNF and TNFR superfamilies. Nat Rev Drug Discov. 2013;12:147–68.
Deng M, Loughran PA, Zhang L, Scott MJ, Billiar TR. Shedding of the tumor necrosis factor (TNF) receptor from the surface of hepatocytes during sepsis limits inflammation through cGMP signalling. Sci Signal. 2015;8:ra11.
Saperstein S, Chen L, Oakes D, Pryhyber G, Finkelstein J. IL-1β augments TNF-α-mediated inflammatory responses from lung epithelial cells. J Interf Cytokine Res. 2009;29:273–84.
Peschon JJ, Slack JL, Reddy P, Stocking KL, Sunnarborg SW, Lee DC, et al. An essential role for ectodomain shedding in mammalian development. Science. 1998;282:1281–4.
Wang J, Al-Lamki RS, Zhang H, Kirkiles-Smith N, Gaeta ML, Thiru S, et al. Histamine antagonizes tumor necrosis factor (TNF) signaling by stimulating TNF receptor shedding from the cell surface and Golgi storage pool. J Biol Chem. 2003;278:21751–60.
Xanthoulea S, Pasparakis M, Kousteni S, Brakebusch C, Wallach D, Bauer J, et al. Tumor necrosis factor (TNF) receptor shedding controls thresholds of innate immune activation that balance opposing TNF functions in infectious and inflammatory diseases. J Exp Med. 2004;200:367–76.
Sakimoto T, Yamada A, Sawa M. Release of soluble tumor necrosis factor receptor 1 from corneal epithelium by TNF-alpha-converting enzyme-dependent ectodomain shedding. Invest Ophthalmol Vis Sci. 2009;50:4618–21.
Giai C, Gonzalez CD, Sabbione F, Garofalo A, Ojeda D, Sordelli DO, et al. Staphylococcus aureus induces shedding of IL-1RII in monocytes and neutrophils. J Innate Immun. 2016;8:284–98.
Aggarwal BB, Gupta SC, Kim JH. Historical perspectives on tumor necrosis factor and its superfamily: 25 years later, a golden journey. Blood. 2012;119:651–65.
Needleman P, Manning PT. Interactions between the inducible cyclooxygenase (COX-2) and nitric oxide synthase (iNOS) pathways: implications for therapeutic intervention in osteoarthritis. Osteoarthr Cartil. 1999;7:367–70.
Meltzer MS, Adams DO, Edelson PJ. Peritoneal mononuclear phagocytes from small animals. In: Methods for studying mononuclear phagocytes: Academic Press New York; 1981. p. 63–7.
Wang C, Yu X, Cao Q, Wang Y, Zheng G, Tan TK, et al. Characterization of murine macrophages from bone marrow, spleen and peritoneum. BMC Immunol. 2013;14:6–16.
Yao L, Berman JW, Factor SM, Lowy FD. Correlation of histopathologic and bacteriologic changes in experimental murine model of bacteremic Staphylococcus aureus infection. Infect Immun. 1997;65:3889–95.
Li Y, Si R, Feng Y, Chen HH, Zou L, Wang E, et al. Myocardial ischemia activates an injurious innate immune signaling via cardiac heat shock protein 60 and toll-like receptor 4. J Biol Chem. 2011;286:31308–19.
Amrani Y, Ammit AJ, Panettieri RA Jr. Tumor necrosis factor receptor (TNFR) 1, but not TNFR2, mediates tumor necrosis factor-α–induced interleukin-6 and RANTES in human airway smooth muscle cells: role of p38 and p42/44 mitogen activated protein kinases. Mol Pharmacol. 2001;60:646–55.
Goto H, Ishihara Y, Kikuchi T, Izawa A, Ozeki N, Okabe E, et al. Interleukin1 receptor antagonist has a novel function in the regulation of MMP13 expression. PLoS One. 2015;10:e0140942.
Becker T, Volchuk A, Rothman JE. Differential use of endoplasmic reticulum membrane for phagocytosis in J774 macrophages. Proc Natl Acad Sci U S A. 2005;102:4022–6.
Lowry OH, Rosenbrough NJ, Farr AL, Randall RJ. Protein measurement with the follin phenol reagent. J Biol Chem. 1951;193:265–75.
Schwarz YA, Amin RS, Stark JM, Trapnell BC, Wilmott RW. Interleukin-1 receptor antagonist inhibits interleukin-8 expression in A549 respiratory epithelial cells infected in vitro with a replication-deficient recombinant adenovirus vector. Am J Respir Cell Mol Biol. 1999;21:388–94.
Nandi A, Bishayi B. A novel CCR-2/TLR-2 triggered signaling in murine peritoneal macrophages intensifies bacterial (Staphylococcus aureus) killing by reactive oxygen species through TNF-R1. Immunol Lett. 2017;190:93–107.
Sherry B, Dai WW, Lesser ML, Trachtman H. Dysregulated chemokine receptor expression and chemokine-mediated cell trafficking in pediatric patients with ESRD. Clin J Am Soc Nephrol. 2008;3:397–406.
Absolom. DR. basic methods for the study of phagocytosis. Methods Enzymol. 1986;132:95–180.
Watanabe I, Ichiki M, Shiratsuchi A, Nakanishi Y. TLR2-mediated survival of Staphylococcus aureus in macrophages: a novel bacterial strategy against host innate immunity. J Immunol. 2007;178:4917–25.
Alves-Filho JC, Freitas A, Souto FO, Spiller F, Paula-Neto H, Silva JS, et al. Regulation of chemokine receptor by Toll-like receptor 2 is critical to neutrophil migration and resistance to polymicrobial sepsis. Proc Natl Acad Sci U S A. 2009;106:4018–23.
Ha H, Debnath B, Neamati N. Role of the CXCL8-CXCR1/2 Axis in Cancer and inflammatory diseases. Theranostics. 2017;7:1543–88.
Iwahashi N, Murakami H, Nimura Y, Takahashi M. Activation of RET tyrosine kinase regulates interleukin-8 production by multiple signaling pathways. Biochem Biophys Res Commun. 2002;294:642–9.
Melehani JH, Duncan JA. Inflammasome activation can mediate tissue-specific pathogenesis or protection in Staphylococcus aureus infection. Curr Top Microbiol Immunol. 2016;397:257–82.
Serhan CN. Inflammation. Signalling the fat controller. Nature. 1996;384:23–4.
Hu YP, Peng YB, Zhang YF, Wang Y, Yu WR, Yao M, et al. Reactive oxygen species mediated prostaglandin E (2) contributes to acute response of epithelial injury. Oxidative Med Cell Longev. 2017;2017:4123854.
Barbieri E, Di Fiore PP, Sigismund S. Endocytic control of signaling at the plasma membrane. Curr Opin Cell Biol. 2016;39:21–7.
Nakamura N, Rabouille C, Watson R, Nilsson T, Hui N, Slusarewicz P, et al. Characterization of a cis-Golgi matrix protein, GM130. J Cell Biol. 1995;131:1715–26.
Cardell LO, Uddman R, Zhang Y, Adner M. Interleukin-1β up-regulates tumor necrosis factor receptors in the mouse airways. Pulm Pharmacol Ther. 2008;21:675–81.
Rodrigues MF, Alves CC, Figueiredo BB, Rezende AB, Wohlres-Viana S, Silva VL, et al. Tumour necrosis factor receptors and apoptosis of alveolar macrophages during early infection with attenuated and virulent Mycobacterium bovis. Immunology. 2013;139:503–12.
Brill A, Chauhan AK, Canault M, Walsh MT, Bergmeier W, Wagner DD. Oxidative stress activates ADAM17/TACE and induces its target receptor shedding in platelets in a p38-dependent fashion. Cardiovasc Res. 2009;84:137–44.
Scott AJ, O'Dea KP, Callaghan DO, Williams L, Dokpesi JO, Tatton L, et al. Reactive oxygen species and p38 mitogen-activated protein kinase mediate tumor necrosis factor α-converting enzyme (TACE/ADAM-17) activation in primary human monocytes. J Biol Chem. 2011;286:35466–76.
Hino T, Nakamura H, Abe S, Saito H, Inage M, Terashita K, et al. Hydrogen peroxide enhances shedding of type I soluble tumor necrosis factor receptor from pulmonary epithelial cells. Am J Respir Cell Mol Biol. 1999;20:122–8.
Gómez MI, Sokol SH, Muir AB, Soong G, Bastien J, Prince AS. Bacterial induction of TNF-alpha converting enzyme expression and IL-6 receptor alpha shedding regulates airway inflammatory signalling. J Immunol. 2005;175:1930–6.
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The author thanks the University Grants Commission (UGC), Government of India, New Delhi, India for funding this project under the University for potential of excellence (UPE) scheme in the UGC UPE II grant: Focus area: Modern Biology Group C2: Mechanistic and therapeutic aspects of infectious diseases (Sanctioned No. UGC/856/UPE-2/MOD BIO/2nd Inst dated 18 Sept 2017). The author is indebted to, Department of Science and Technology, Government of India for providing us with the instruments procured under the DST-PURSE programme to the Department of Physiology, University of Calcutta.
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Dutta, P., Sultana, S., Dey, R. et al. Regulation of Staphylococcus aureus-induced CXCR1 expression via inhibition of receptor mobilization and receptor shedding during dual receptor (TNFR1 and IL-1R) neutralization. Immunol Res 67, 241–260 (2019). https://doi.org/10.1007/s12026-019-09083-x
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DOI: https://doi.org/10.1007/s12026-019-09083-x