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Sevoflurane Combined with ATP Activates Caspase-1 and Triggers Caspase-1-Dependent Pyroptosis in Murine J774 Macrophages

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Abstract

Sevoflurane is one of the most commonly used volatile anesthetics. Recent studies have shown that sevoflurane plays an important role in modulation of inflammation and immunity. However, little is known about the related molecular mechanisms. This study was designed to investigate the effects and mechanisms of sevoflurane on inflammatory cell death pyroptosis in the murine macrophage cell line J774 cells. Sevoflurane combined with ATP could increase the level of activated caspase-1, pyroptosis, and reactive oxygen species (ROS). Furthermore, treatment of cells with the caspase-1 inhibitor Ac-YVAD-CMK dramatically decreased the percentage of pyroptosis. In addition, inhibition of ROS with N-acetyl-l-cysteine or diphenyleneiodonium significantly reduced the activated levels of caspase-1. These results demonstrated that sevoflurane combined with ATP could activate caspase-1 and trigger caspase-1-dependent pyroptosis through the modulation of ROS production.

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ACKNOWLEDGMENTS

The authors thank the Major Program of National Natural Science Foundation of China (81130036) and National Science Fund for Distinguished Young Scholars from National Natural Science Foundation of China (30825037).

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Authors and Affiliations

  1. Department of Anesthesiology, The First Affiliated Hospital, School of Medicine, Zhejiang University, QingChun Road 79, 310003, Hangzhou, China

    Yue Jin, Hui Li, Guohao Xie, Shuijin Wu & Xiangming Fang

  2. Laboratory of Molecular Cell Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes of Biological Sciences, Chinese Academy of Sciences, Shanghai, 200031, China

    Shuzhen Chen

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  1. Yue Jin
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  2. Hui Li
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  3. Guohao Xie
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  4. Shuzhen Chen
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Correspondence to Xiangming Fang.

Additional information

Yue Jin and Hui Li contributed equally to this work.

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Jin, Y., Li, H., Xie, G. et al. Sevoflurane Combined with ATP Activates Caspase-1 and Triggers Caspase-1-Dependent Pyroptosis in Murine J774 Macrophages. Inflammation 36, 330–336 (2013). https://doi.org/10.1007/s10753-012-9550-6

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  • DOI: https://doi.org/10.1007/s10753-012-9550-6

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