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Minocycline Treatment and Bone Marrow Mononuclear Cell Transplantation After Endothelin-1 Induced Striatal Ischemia

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Abstract

We explored whether the modulation of microglia activation with minocycline is beneficial to the therapeutic actions of bone marrow mononuclear cells (BMMCs) transplanted after experimental stroke. Male Wistar adult rats were divided in four experimental groups: ischemic control saline treated (G1, N = 6), ischemic minocycline treated (G2, N = 5), ischemic BMMC treated (G3, N = 5), and ischemic minocycline/BMMC treated (G4, N = 6). There was a significant reduction in the number of ED1+ cells in G3 animals (51.31 ± 2.41, P < 0.05), but this effect was more prominent following concomitant treatment with minocycline (G4 = 29.78 ± 1.56). There was conspicuous neuronal preservation in the brains of G4 animals (87.97 ± 4.27) compared with control group (G1 = 47.61 ± 2.25, P < 0.05). The behavioral tests showed better functional recovery in animals of G2, G3, and G4, compared with G1 and baseline (P < 0.05). The results suggest that a proper modulation of microglia activity may contribute to a more permissive ischemic environment contributing to increased neuroprotection and functional recovery following striatal ischemia.

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Acknowledgments

This work was supported by the Brazilian National Council for Scientific and Technological Development (CNPq) and Fundação de Amparo A Pesquisa do Estado do Pará (FAPESPA). W Gomes-Leal is a principal investigator in grant number 573872/2008-2 from the Ministry of Science and Technology (MCT), Ministry of Health (MS), and CNPq (Edital CT-Biotecnologia/MCT/CNPq/MS/SCTIE/DECIT No. 17/2008) and FAPESPA (PRONEX-FAPESPA-CNPQ-Edital 012-2009).

Disclosures/Conflict of Interest

The authors declare no conflict of interest.

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Authors and Affiliations

  1. Laboratory of Experimental Neuroprotection and Neuroregeneration, Institute of Biological Sciences, Federal University of Pará-Brazil, Rua Augusto Corrêa S/N, Campus do Guamá, 66075-900, Belém, Pará, Brazil

    Marcelo M. Cardoso, Edna C. S. Franco, Celice C. de Souza, Michelle C. da Silva & Walace Gomes-Leal

  2. Theory and Research of Behavior Nucleus, Federal University of Pará, Belém, Brazil

    Edna C. S. Franco

  3. Technological Innovation Center, Arbovirus and Hemorrhagic Fever Department, Evandro Chagas Institute (IEC-SVS/MS), Rodovia BR-316 km 7 s/n Levilândia, 670300-000, Ananindeua, Pará, Brazil

    Amauri Gouveia

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  1. Marcelo M. Cardoso
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Correspondence to Walace Gomes-Leal.

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Cardoso, M.M., Franco, E.C.S., de Souza, C.C. et al. Minocycline Treatment and Bone Marrow Mononuclear Cell Transplantation After Endothelin-1 Induced Striatal Ischemia. Inflammation 36, 197–205 (2013). https://doi.org/10.1007/s10753-012-9535-5

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