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PnF3H, a flavanone 3-hydroxylase from the Antarctic moss Pohlia nutans, confers tolerance to salt stress and ABA treatment in transgenic Arabidopsis

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Abstract

Flavonoids are widely distributed secondary metabolites in plants. However, far less research has been carried out on genes involved in the flavonoid biosynthetic pathways in bryophytes. Here, a novel flavanone 3-hydroxylase gene (PnF3H) was cloned from Antarctic moss Pohlia nutans. Subcellular localization revealed that PnF3H were mainly distributed to cytosol. The expression of PnF3H was rapidly induced by salt, UV-B, cold and drought stresses as well as by exogenously applied abscisic acid (ABA). Transgenic Arabidopsis plants of overexpressing PnF3H exhibited enhanced tolerance to salt and oxidative stresses. The expression levels of several stress resistance genes (i.e., AtSOS3, AtP5CS1, AtHKT1, AtCAT1 and AtAPX1) were markedly up-regulated in transgenic plants. The activities of antioxidant enzymes were increased, while the content of hydrogen peroxide was obviously decreased in PnF3H transgenic plants. Meanwhile, the expression levels of ABA-responsive genes which confer for controlling seeds germination, such as AtABI4, AtABI5, AtABI3 and AtABF3 were markedly decreased. Additionally, overexpressing PnF3H alleviated the inhibitory effects of naringenin on plant growth and changed the flavonoid components in transgenic plants. Therefore, we proposed that PnF3H was a flavanone 3-hydroxylase involving in regulating the salinity tolerance and ABA sensitivity to enable P. nutans to adapt to polar climates.

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Acknowledgements

This work was supported by the National Natural Science Foundation of China (41206176 and 41476174), Natural Science Foundation of Shandong Province (ZR2014DQ012), Basic Scientific Fund for National Public Research Institutes of China (2014T04), and Excellent Creative Team Fund of Jinan.

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Authors and Affiliations

  1. National Glycoengineering Research Center and School of Life Science, Shandong University, Jinan, 250100, China

    Chengcheng Li, Xinghao Yao, Jing Wang, Tailin Wang, Pengying Zhang & Kaoshan Chen

  2. Marine Ecology Research Center, The First Institute of Oceanography, State Oceanic Administration, Qingdao, 266061, China

    Shenghao Liu & Zhaohui Zhang

  3. Shandong Provincial Key Laboratory of Carbohydrate Chemistry and Glycobiology, Jinan, 250100, China

    Chengcheng Li, Xinghao Yao, Tailin Wang, Pengying Zhang & Kaoshan Chen

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  1. Chengcheng Li
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Correspondence to Pengying Zhang.

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Li, C., Liu, S., Yao, X. et al. PnF3H, a flavanone 3-hydroxylase from the Antarctic moss Pohlia nutans, confers tolerance to salt stress and ABA treatment in transgenic Arabidopsis . Plant Growth Regul 83, 489–500 (2017). https://doi.org/10.1007/s10725-017-0314-z

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