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Relationships between endogenous hormonal content and direct somatic embryogenesis in Prunus persica L. Batsch cotyledons

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Abstract

Cotyledons of peach (Prunus persica L. Batsch cv. ZiseMay®) were cultured in vitro on medium deprived of plant growth regulators. Two different lines varying in their embryogenic capacity were studied after 90 days in culture media. Endogenous levels of abscisic acid (ABA), indole-3-acetic acid (IAA), trans-zeatin (Z), trans-zeatin riboside (ZR), the ethylene precursor 1-aminocyclopropane-1-carboxylic acid (ACC), salicylic acid (SA), and jasmonic acid (JA) were analyzed in embryogenic and non-embryogenic cotyledons. No significant differences were observed in total ABA, IAA, ZR, SA and JA concentrations between the embryogenic and non-embryogenic cotyledons. On the contrary, lower Z and ACC contents, and also a reduced balance between Z and IAA levels were related with the embryogenic capacity of the cotyledons. These results suggest that the difference in somatic embryo formation capacity observed between embryogenic and non-embryogenic cotyledons is related to their endogenous Z contents, and that the endogenous hormonal balance between Z and IAA is an important index defining the embryogenic potential in peach cotyledons.

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Acknowledgments

The authors gratefully acknowledge Dr Francisco Pérez Alfocea for his thorough review and suggestions to improve the manuscript and his generosity. This report was supported by the Instituto Nacional de Investigaciones Agrarias (INIA) (RTA2008-00121-00-00) and by a fellowship also provided by INIA to Margarita Pérez-Jiménez.

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Authors and Affiliations

  1. Departamento de Horticultura, Instituto Murciano de Investigación y Desarrollo Agrario y Alimentario (IMIDA), C/Mayor s/n, 30150, La Alberca, Murcia, Spain

    Margarita Pérez-Jiménez & José Cos-Terrer

  2. Departamento de Nutrición Vegetal, Centro de Edafología y Biología Aplicada del Segura (CEBAS), Consejo Superior de Investigaciones Científicas (CSIC), Campus Universitario de Espinardo, 30100, Murcia, Spain

    Elena Cantero-Navarro

  3. Departamento de Biología Vegetal, Facultad de Biología, Universidad de Murcia, Campus Universitario de Espinardo, 30100, Murcia, Spain

    Manuel Acosta

Authors
  1. Margarita Pérez-Jiménez
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  2. Elena Cantero-Navarro
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  3. Manuel Acosta
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  4. José Cos-Terrer
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Correspondence to Margarita Pérez-Jiménez.

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Pérez-Jiménez, M., Cantero-Navarro, E., Acosta, M. et al. Relationships between endogenous hormonal content and direct somatic embryogenesis in Prunus persica L. Batsch cotyledons. Plant Growth Regul 71, 219–224 (2013). https://doi.org/10.1007/s10725-013-9822-7

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  • DOI: https://doi.org/10.1007/s10725-013-9822-7

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