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Physiological, biochemical, and molecular characterization of a new female sterile mutant in turnip

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Abstract

A female sterile plant with aborted pistils was previously obtained in an advanced self-pollinated turnip inbred line. Cross progeny with normal wild-type (WT) plants successfully characterized the mechanism of pistil abortion, and the mutant was designated ‘tpa’ (turnip pistil abortion). Further morphological investigations showed that the tpa mutant included two phenotypes, pistil abortion partially (PAP) and pistil abortion completely (PAC), and our recent result showed that the tpa trait was controlled by two recessive genes (Wu et al. 2011). In this study, comparisons of the floral organs indicated that corolla size and petal, sepal, filament, and anther length measurements did not significantly differ between the tpa and WT plants. Subsequently, the activities of enzymes (catalase, ascorbate peroxidase, and guaiacol peroxidase) related to the metabolism of reactive oxygen, the O −2 production rate, as well as the contents of hydrogen peroxide and malondialdehyde were tested and found to be generally higher in tpa floral organs than in the WT. The reverse was true for the flower stems, however. Quantitative differences in peroxidase, cytochrome oxidase, and esterase isoenzyme patterns were also detected among these different organs. These findings suggest that pistil abortion is probably correlated with the unbalanced homeostasis of ROS antioxidant enzymes. Moreover, a specific band was recognized exclusively in the tpa plants using random amplified polymorphic DNA analysis. Sequencing and blasting results indicated that the 878-bp DNA fragment was located in the D1/D2/D3 region, near the 5′ end of the 26S rDNA. This study valuably adds to the current knowledge of and will help further elucidate the biological mechanism of female sterility induction.

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Abbreviations

APX:

Ascorbate peroxidase

CAT:

Catalase

COD:

Cytochrome oxidase

EDTA:

Ethylenediaminetetraacetic acid

EST:

Esterase

GPX:

Guaiacol peroxidase

H2O2 :

Hydrogen peroxide

MDA:

Malondialdehyde

O −2 :

Superoxide anion radical

PAC:

Pistil aborted completely

PAGE:

Polyacrylamide gel electrophoresis

PAP:

Pistil aborted partially

POD:

Peroxidase

RAPD:

Random amplified polymorphic DNA

rDNA:

Ribosomal DNA

ROS:

Reactive oxygen species

TBA:

2-thiobarbituric acid

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Acknowledgments

This work was supported by the Nature Science Foundation of China (Grant 30771377), the Nature Science Foundation of Zhejiang Province (Grant Y3090294), and the Sci-Tech Project of Zhejiang Province (Grant 2009C32029).

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Authors and Affiliations

  1. Laboratory of Cell and Molecular Biology, Institute of Vegetable Science, Zhejiang University, 866 Yuhangtang Road, Hangzhou, 310058, Zhejiang, China

    Zhenning Liu, Xiaolin Yu, Fangzhan Wang, Shuai Hu, Yapei Liu & Gang Lu

  2. Key Laboratory of Horticultural Plant Growth, Development, and Quality Improvement, Ministry of Agriculture, Hangzhou, 310058, Zhejiang, China

    Zhenning Liu, Xiaolin Yu, Fangzhan Wang, Shuai Hu, Yapei Liu & Gang Lu

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  1. Zhenning Liu
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  2. Xiaolin Yu
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  3. Fangzhan Wang
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  4. Shuai Hu
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  5. Yapei Liu
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Correspondence to Xiaolin Yu.

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Liu, Z., Yu, X., Wang, F. et al. Physiological, biochemical, and molecular characterization of a new female sterile mutant in turnip. Plant Growth Regul 68, 239–248 (2012). https://doi.org/10.1007/s10725-012-9712-4

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