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Water deficit-induced oxidative stress and the activation of antioxidant enzymes in white clover leaves

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  • Published:
Biologia Plantarum

Abstract

The objective of this study was to determine the development of the antioxidant enzymes induced by drought stress in white clover (Trifolium repens L.) leaves. Water stress was imposed during 28 d by decreasing the daily irrigation. Leaf water potential (Φw) gradually decreased from −0.46 to −2.33 MPa. For the first 7 d, dry mass (DM), H2O2 and lipid peroxidation were not significantly affected by water deficit. From 14 d of treatment, water stress decreased dry mass and increased content of reactive oxygen species (O2 ·− and H2O2) and oxidative stress (malondialdehyde content). The ascorbate peroxidase (APOD) was activated most rapidly, already during the first week of water stress, but then its activity slowly decreased. Activation of superoxide dismutase (SOD) and catalase (CAT) by water deficit continued during the 14 d (Φw ≥ −1.65 MPa) and then their activities remain on the similar level. The activity of guaiacol-peroxidase (GPOD) increased mostly under progressive water stress and was correlated with increase in lipid peroxidation and growth restriction.

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Abbreviations

APOD:

ascorbate peroxidase

CAT:

catalase

DM:

dry mass

GPOD:

guaiacol peroxidase

MDA:

malondialdehyde

NBT:

nitroblue tetrazolium

ROS:

reactive oxygen species

RWC:

relative water content

SOD:

superoxide dismutase

TEMED:

N,N,Ń,Ń-tetramethylethylenediamine

TBA:

thiobarbituric acid

TCA:

trichloroacetic acid

ε:

coefficient of absorbance

Φw :

leaf water potential

References

  • Asada, K.: Ascorbate peroxidase — a hydrogen peroxide scavenging enzyme in plant. — Physiol. Plant. 85:235–241, 1992.

    Article  CAS  Google Scholar 

  • Beauchamp, C., Fridovich, I.: Superoxide dismutase: improved assays and assay applicable to acrylamide gels. — Anal. Biochem. 44:276–287, 1971.

    Article  CAS  PubMed  Google Scholar 

  • Bradford, M.M.: A rapid sensitive method for the quantification of microgram quantities of protein utilizing the principle of proteins-dye binding. — Anal. Biochem. 72:248–254, 1976.

    Article  CAS  PubMed  Google Scholar 

  • Cakmak, I., Horst, J.H.: Effects of aluminum on lipid peroxidation, superoxide dismutase, catalase, and peroxidase activities in root tips of soybean (Glycine max). — Physiol. Plant. 83:463–468, 1991.

    Article  CAS  Google Scholar 

  • Caruso, C., Chilosi, G., Caporale, C., Leonardi, L., Bertini, L., Magro, P., Buonocore, V.: Induction of pathogenesis-related proteins in germinating wheat seeds infected with Fusarium culmorum. — Plant. Sci. 140:107–120, 1999.

    Article  Google Scholar 

  • Cavalcanti, F.R., Oliveira, J.T.A., Martins-Miranda, A.S., Viégas, R.A., Silvira, J.A.G.: Superoxide dismutase, catalase and peroxidase activities do not confer protection against oxidative damage in salt-stressed cowpea leaves. — New Phytol. 163:563–571, 2004.

    Article  CAS  Google Scholar 

  • Chazen, O, Neumann, P.M.: Hydraulic signals from roots and rapid cell-wall hardening in growing maize (Zea mays L.) leaves are primary responses to polyethylene glycol-induced water deficits. — Plant Physiol. 104:1385–1392, 1994.

    CAS  PubMed  Google Scholar 

  • Chen, G., Asada, K.: Ascorbate peroxidase in tea leaves: Occurrence of two isozymes and the differences in their enzymatic and molecular properties. — Plant Cell Physiol. 30:987–998, 1989.

    CAS  Google Scholar 

  • Chen, Y.A., Shin, J.W., Liu, Z.H.: Effect of light on peroxidase and lignin synthesis in mungbean hypocotyls. — Plant Physiol. Biochem. 40:33–39, 2002.

    Article  CAS  Google Scholar 

  • Costa França, M.G., Thi, A.T.P., Pimntel, C., Pereyra Rossiello, R.O., Zuily-Fodil, Y., Laffray, D.: Differences in growth and water relations among Phaseolus vulgaris cultivars in response to induced drought stress. — Environ. exp. Bot. 43:227–237, 2000.

    Article  PubMed  Google Scholar 

  • Foyer, C.H., Lopez-Delgado, H., Dat, J.F., Scott, I.M.: Hydrogen peroxide- and glutathione-associated mechanisms of acclamatory stress tolerance and signaling. — Physiol. Plant. 100:241–254, 1997.

    Article  CAS  Google Scholar 

  • Foyer, C.H., Noctor, G.: Oxygen processing in photosynthesis: regulation and signaling. — New Phytol. 146:359–388, 2000.

    Article  CAS  Google Scholar 

  • Foyer, C.H., Noctor, G.: Redox sensing and signaling associated with reactive oxygen in chloroplasts, peroxisomes and mitochondria. — Physiol. Plant. 119:355–364, 2003.

    Article  CAS  Google Scholar 

  • Foyer, C.H., Valadier, M.H., Andre, M., Becker, T.W.: Drought-induced effects on nitrate reductase activity and mRNA and on the coordination of nitrogen and carbon metabolism in maize leaves. — Plant Physiol. 117:283–292, 1998.

    Article  CAS  PubMed  Google Scholar 

  • Giannopolities, C.H., Ries, S.K.: Superoxide dismutase. I. Occurrence in higher plant. — Plant Physiol. 59:309–314, 1977.

    Article  Google Scholar 

  • Jiang, Y., Huang, B.: Drought and heat stress injury to two cool-season turfgrasses in relation to antioxidant metabolism and lipid peroxidation. — Crop Sci. 41:436–442, 2001.

    Article  CAS  Google Scholar 

  • Jung, S.: Variation in antioxidant metabolism of young and mature leaves of Arabidopsis thaliana subjected to drought. — Plant Sci. 166:459–466, 2004.

    Article  CAS  Google Scholar 

  • Kim, T.H., Lee, B.R., Jung, W.J., Kim, K.Y., Avice, J.C., Ourry, A.: De novo protein synthesis in relation to ammonia and proline accumulation in water stressed white clover. — Funct. Plant Biol. 31:847–855, 2004.

    Article  CAS  Google Scholar 

  • Lee, T.M., Lin, Y.H.: Changes in soluble and cell wall-bound peroxidase activities with growth in anoxia-treated rice (Oryza sativa L.) coleoptiles and roots. — Plant Sci. 106:1–7, 1995.

    Article  CAS  Google Scholar 

  • Lin, C.C., Kao, C.H.: Cell wall peroxidase activity, hydrogen peroxidase level and NaCl-inhibited root growth of rice seedling. — Plant Soil 230:135–143, 2001.

    Article  CAS  Google Scholar 

  • Lin, C.L., Kao, C.H.: Osmotic stress-induced changes in cell wall peroxidase activity and hydrogen peroxide level in roots of rice seedlings. — Plant Growth Regul, 37:177–184, 2002.

    Article  CAS  Google Scholar 

  • Mishra, N.P., Mishra, R.K., Singhal, G.S.: Changes in the activities of antioxidant enzymes during exposure of intact wheat leaves of strong visible light at different temperature in the presence of protein synthesis inhibitors. — Plant Physiol. 102:903–910, 1993.

    CAS  PubMed  Google Scholar 

  • Peltzer, D., Dreyer, E., Polle, A.: Differential temperature dependencies of antioxidative enzymes in two contrasting species: Fagus sylvatica and Coleus blumei. — Plant Physiol. Biochem. 40:141–150, 2002.

    Article  CAS  Google Scholar 

  • Rao, M.V., Paliyath, G., Ormrod, D.P.: Ultraviolet-B- and ozone-induced biochemical changes in antioxidant enzymes of Arabidopsis thaliana. — Plant Physiol. 110:125–136, 1996.

    Article  CAS  PubMed  Google Scholar 

  • Selote, D.S., Bharti, S., Khanna-Chopra, R.: Drought acclimation reduces O2 ·− accumulation and lipid peroxidation in wheat seedlings. — Biochem. biophys. Res. Commun. 314:724–729, 2004.

    Article  CAS  PubMed  Google Scholar 

  • Silva, H.A.S., Romeiro, R.S., Macagnan, D., Halfeld-Vieira, B.A., Pereira, M.C.B., Mounteer, A.: Rhizobacterial induction of systemic resistance in tomato plants: nonspecific protection a decrease in enzyme activities. — Biol. Control 29:288–295, 2004.

    Article  CAS  Google Scholar 

  • Sivritepe, N., Erturk, U., Yerlikaya, C., Turkan, I., Bor, M., Ozdemir, F.: Response of cherry rootstock to water stress induced in vitro. — Biol. Plant. 52:573–576, 2008.

    Article  CAS  Google Scholar 

  • Smirnoff, N.: The role of active oxygen in the response of plants to water deficit and desiccation. — New Phytol. 125:27–58, 1993.

    Article  CAS  Google Scholar 

  • Vaidyanathan, H., Sivakumar, P., Chakrabarty, R., Thomas, G.: Scavenging of reactive oxygen species in NaCl-stressed rice (Oryza sativa L.) — differential response in salt-tolerant and sensitive varieties. — Plant Sci. 165:1411–1418, 2003.

    Article  CAS  Google Scholar 

  • Wang, A.G., Luo, G.H.: Quantitative relation between the reaction of hydroxylamine and superoxide anion radicals in plants. — Plant Physiol. Commun. 6:55–57, 1990.

    CAS  Google Scholar 

  • Woodbury, W., Spencer, A.K., Stahmann, M.A.: Improved procedure using ferricyanide for detecting catalase isozymes. — Anal. Biochem. 44:301–305, 1971.

    Article  CAS  PubMed  Google Scholar 

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Authors and Affiliations

  1. Department of Animal Science, Environmental-Friendly Agriculture Research Center, Chonnam National University, Gwangju, 500-757, Korea

    B. R. Lee, L. S. Li & T. H. Kim

  2. Department of Biological and Environmental Chemistry, Environmental-Friendly Agriculture Research Center, Chonnam National University, Gwangju, 500-757, Korea

    W. J. Jung

  3. College of Plant Protection, Qingdao Agriculture University, Qingdao City, Shandong, P.R. China

    Y. L. Jin

  4. Institut de Biologie Fondamentale et Appliquée, Université de Caen, F-14032, Caen Cedex, France

    J. C. Avice & A. Ourry

Authors
  1. B. R. Lee
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  2. L. S. Li
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  3. W. J. Jung
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  4. Y. L. Jin
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  5. J. C. Avice
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  6. A. Ourry
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  7. T. H. Kim
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Correspondence to T. H. Kim.

Additional information

Acknowledgements: This work was supported by the Technology Development Program for Agriculture and Forestry, Ministry of Agriculture and Forestry, Republic of Korea, through the Environmental-Friendly Agriculture Research Center at Chonnam National University.

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Lee, B.R., Li, L.S., Jung, W.J. et al. Water deficit-induced oxidative stress and the activation of antioxidant enzymes in white clover leaves. Biol Plant 53, 505–510 (2009). https://doi.org/10.1007/s10535-009-0091-2

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  • DOI: https://doi.org/10.1007/s10535-009-0091-2

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