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Polymorphism of Got2 DNA sequences sheds light on Aegilops tauschii Coss. intraspecies divergence and origin of Triticum aestivum L.

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Abstract

DNA sequences of nuclear gene Got2 was studied in 60 accessions of Aegilops tauschii, 29 of subsp. tauschii and 31 of subsp. strangulata. It was found that Got2 allozyme polymorphism in Ae. tauschii is due to a single, unique, mutation which led to replacement of glutamic acid by isoleucine in residue 256 of the enzyme molecule, encoded by Got2. As revealed by Got2 DNA sequences variation, initially in its history Ae. tauschii was presented by subsp. strangulata, and among phylogenetic lineages of subsp. strangulata, the lineage “t-91s” (TauL3) is the most ancient, a relict one. Subspecies tauschii is relatively “young”. Initially it was presented by the lineage marked by combination of allozyme alleles Got2 105 and Acph1 100. In the past it inhabited the Continental area from Caucasia to Pakistan, but later on it was forced out by newly originated, now—a major lineage of subsp. tauschii, marked by Got2 100. This lineage extended the Continental area of the species up to Kirgizstan, but actually failed to penetrate into pre-Caspian area, occupied by subsp. strangulata. These results essentially differ from those obtained previously, using chloroplast DNA (cpDNA) sequences polymorphism. As revealed by cpDNA, the major, “usual”, subsp. strangulata (TauL2) is “younger” than subsp. tauschii, which resided on phylogenetic tree between relict lineage “t-91s”of subsp. strangulata—and major subsp. strangulata. But both cpDNA and Got2 DNA sequences indicate that the level of genetic variation in subsp. tauschii is much lower than in subsp. strangulata. According to Got2 DNA sequences variation, it was Ae. tauschii subsp. strangulata lineage “k-109″ which donated genome D to Triticum aestivum L. This lineage includes accessions: k-109 from South-Eastern Precaspian Azerbaijan; KU-2105, KU-2159 from Western Precaspian Iran; KU-2080 from Eastern Precaspian Iran.

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Acknowledgments

I wish to express my sincere gratitude to Dr. Vera S. Bogdanova, Dr. Aleksey V. Katokhin and Prof. Elena K. Khlestkina for the help in the course of this study. The work was supported by Russian Foundation for Basic Research, Grant 16-04-00106-a, and RF Basic project 0324-2015-0004.

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  1. Institute of Cytology and Genetics, Novosibirsk, Russia, 630090

    Alexander Ju. Dudnikov

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Correspondence to Alexander Ju. Dudnikov.

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Fig. 1s

(supplement) Amino-acid sequences of the enzyme glutamatic-oxaloacetatic transaminase, encoded by Got2, in 60 Aegilops tauschii accessions (GIF 35 kb)

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Dudnikov, A.J. Polymorphism of Got2 DNA sequences sheds light on Aegilops tauschii Coss. intraspecies divergence and origin of Triticum aestivum L.. Genet Resour Crop Evol 64, 1623–1640 (2017). https://doi.org/10.1007/s10722-016-0461-5

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