Abstract
Coix is a genus in the grass family placed in the tribe Maydeae. It is closely related to maize and is also used as a crop plant. Since many valuable traits have been identified recently in Coix, it is considered to be a valuable genetic resource, particularly for maize improvement. In this study, a Coix genetic linkage map was constructed using an F2 population of 131 individuals. Eighty AFLP and 10 RFLP markers were mapped, covering a total length of 1339.5 cM with an average interval of 14.88 cM. The map consisted of 10 linkage groups, were consistent with the chromosome numbers observed cytogenetically. Both AFLP and RFLP markers were used first for genetic analysis in Coix. AFLP markers were generated by two restriction enzyme combinations, EcoRI/MseI and PstI/MseI. A total of 1349 bands were amplified, of which 140 were polymorphic. The polymorphism detection efficiency of the two enzyme combinations was compared, and utility of AFLP markers to construct the linkage map was discussed. Ten RFLP markers detected by three different probes were distributed on eight different linkage groups. The results provide a foundation to map and isolate important genes in Coix, and to investigate its genomic architecture, possible origins, and relationship with maize at the DNA level.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
S. Ahn S.D. Tanksley (1993) ArticleTitleComparative linkage maps of the rice and maize genomes Proc. Natl. Acad. Sci. USA 90 7980–7984
C. Alonso-Blanco A.J. Peeters M. Koornneef C. Lister C. Dean N. Van den Bosch J. Pot M.T. Kuiper (1998) ArticleTitleDevelopment of an AFLP-based linkage map of Ler, Col and Cvi Arabidopsis thaliana ecotypes and construction of a Ler/Cvi recombinant inbred line population Plant J. 14 259–271
P. Castiglioni P. Ajmone-Marsam R. Van Wijk (1999) ArticleTitleAFLP markers in a molecular linkage map of maize: Codominant scoring and linkage group distribution Theor. Appl. Genet. 99 425–431
P. Castiglioni C. Pozzi M. Heun V. Terzi K.J. Muller W. Rohde F. Salamini (1998) ArticleTitleAn AFLP-based procedure for the efficient mapping of mutations and DNA probes in barley Genetics 149 2039–2056
I. Eujaye M. Baum W. Powell (1998) ArticleTitleA genetic linkage map of lentil (Lens sp.) based on RAPD and AFLP markers using recombinant inbred lines Theor. Appl. Genet. 97 83–89
M.D. Gale K.M. Devos (1998) ArticleTitlePlant comparative genetics after 10 years Science 282 656–659
J.M. Gardiner E.H. Coe S. Metia-Hancock D.A. Hoisington S. Chao (1993) ArticleTitleDevelopment of a long RFLP map in maize using an immortalized F2 population Genetics 134 917–930
A.R. Hallauer J.B. Miranda (1981) Quantitative Genetics in Maize Breeding Iowa State University Press Ames IA 373–405
T. Helentjaris M. Slocum S. Wright A. Schaefer J. Nienhuis (1986) ArticleTitleConstruction of genetic linkage maps in maize and tomato using restriction fragment length polymorphism. Theor. Appl. Genet. 72 761–769
H. Huang (1995) ArticleTitleEcotypes, diversities and utilization of the coix varieties from China Crop Germplasm 4 4–8
D. Kevin V.K. Livingstone J.R. Lockney R.W. Blauth K.J. Molly (1999) ArticleTitleGenome mapping in Capsicum and evolution of genome structure in the Solanaceae Genetics 152 1183–1202
D.D. Kosambi (1994) ArticleTitleThe estimation of map distances from recombination values Ann. Eugen. 12 172–175
E.S. Lander P. Green J. Abrahamson A. Barlow M.J. Daly S.E. Lincoln L. Newburn (1987) ArticleTitleMAPMAKER: an interactive computer package for constructing primary genetic linkage maps of experimental and natural populations Genomics 1 174–181
X.H. Li Y.Q. Huang J.S. Li H. Corke (2001) ArticleTitleCharacterization of genetic variation and relationships among Coix germplasm accessions using RAPD markers Genet. Resour. Crop Evol 48 189–194
Z.X. Lu D. Sosinski G.L. Reighard W.V. Baird A.G. Abbott (1998) ArticleTitleConstruction of a genetic linkage map and identification of AFLP markers for resistance to root- knot nematodes in peach rootstocks Genome 41 199–207
M. Maheswaran P.K. Subudhi S. Nandi J.C. Xu A. Parco D.C. Yang N. Huang (1997) ArticleTitlePolymorphism, distribution, and segregation of AFLP markers in a doubled-haploid rice population Theor. Appl. Genet 94 39–45
M.A. Saghai-Maroof G.P. Yang R.M. Biyashev P.J. Maughan Q. Zhang (1984) ArticleTitleAnalysis of the barley and rice genomes by comparative RFLP linkage mapping Theor. Appl. Genet. 92 541–551
M.H. Tixier R.M. Sourdille P. Leroy M. Bernard (1997) ArticleTitleDetection of wheat microsatellites using a non-radioactive silver-nitrate staining method J. Genet. Breed. 51 175–177
P. Vos R. Hogers M. Bleeker M. Reijans T. Van de Lee M. Hornes A. Friiters J. Pot J. Peleman M. Kuiper M. Zabeau (1995) ArticleTitleAFLP: a new technique for DNA fingerprinting Nucleic Acids Res. 23 4407–4414
L.Z. Xiong S.P. Wang K.D. Liu X.K. Dai M.A. Saghai- Maroof J.G. Hu Q.F. Zhang (1998) ArticleTitleDistribution of simple sequence repeat and AFLP markers in molecular linkage map of rice Acta Bot. Sin. 7 605–614
T.D. Zhuang Z.H. Pan X.M. Yao (1994) ArticleTitleGenetic variation and karyotypic evolution of Coix in China J. Plant Resour. Environ. 3 16–21
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Qin, F., Li, J., Li, X. et al. AFLP and RFLP linkage map in Coix. Genet Resour Crop Evol 52, 209–214 (2005). https://doi.org/10.1007/s10722-003-1387-2
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/s10722-003-1387-2