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Genetic mapping and QTL analysis of agronomic traits in Indian Mucuna pruriens using an intraspecific F2 population

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Abstract

Mucuna pruriens is a well-recognized agricultural and horticultural crop with important medicinal use. However, antinutritional factors in seed and adverse morphological characters have negatively affected its cultivation. To elucidate the genetic control of agronomic traits, an intraspecific genetic linkage map of Indian M. pruriens has been developed based on amplified fragment length polymorphism (AFLP) markers using 200 F 2 progenies derived from a cross between wild and cultivated genotypes. The resulting linkage map comprised 129 AFLP markers dispersed over 13 linkage groups spanning a total distance of 618.88 cM with an average marker interval of 4.79 cM. For the first time, three QTLs explaining about 6.05–14.77% of the corresponding total phenotypic variation for three quantitative (seed) traits and, eight QTLs explaining about 25.96% of the corresponding total phenotypic variation for three qualitative traits have been detected on four linkage groups. The map presented here will pave a way for mapping of genes/QTLs for the important agronomic and horticultural traits contrasting between the parents used in this study.

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Acknowledgements

The authors acknowledge financial support from the Department of Science and Technology (DST) and Department of Biotechnology (DBT), Govt. of India for this project. NS thank Sikkim University, Gangtok and Sri Krishnadevaraya Educational Trust (Sri KET), Bangalore, India for the laboratory and field facilities.

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Authors and Affiliations

  1. Department of Biotechnology, Sir M. Visvesvaraya Institute of Technology, Hunasamaranahalli, Bengaluru, 562 157, India

    S. MAHESH, M. LEELAMBIKA & MD JAHEER

  2. Department of Plant Breeding, Wagenigen University and Research Centre, 6708 PB, Wageningen, The Netherlands

    A. M. ANITHAKUMARI

  3. Department of Botany, Sikkim University, Gangtok, Tadong, 737 102, India

    N. SATHYANARAYANA

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  1. S. MAHESH
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  2. M. LEELAMBIKA
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  4. A. M. ANITHAKUMARI
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  5. N. SATHYANARAYANA
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Correspondence to N. SATHYANARAYANA.

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[Mahesh S., Leelambika M., Jaheer M., Anithakumari A. M. and Sathyanarayana N. 2016 Genetic mapping and QTL analysis of agronomic traits in Indian Mucuna pruriens using an intraspecific F2 population. J. Genet. 95, xx–xx]

MS and LM raised mapping population, carried out marker analysis and QTL mapping. MJ assisted in field and laboratory works. AAM reviewed the manuscript. NS conceptualized the work and obtained funding

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MAHESH, S., LEELAMBIKA, M., JAHEER, M. et al. Genetic mapping and QTL analysis of agronomic traits in Indian Mucuna pruriens using an intraspecific F2 population. J Genet 95, 35–44 (2016). https://doi.org/10.1007/s12041-015-0602-1

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