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Genetic diversity of avenin-like b genes in Aegilops tauschii Coss

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Abstract

Avenin-like storage proteins influence the rheological properties and processing quality in common wheat, and the discovery of new alleles will benefit wheat quality improvement. In this study, 13 avenin-like b alleles (TaALPb7D-A–M) were discovered in 108 Aegilops tauschii Coss. accessions. Ten alleles were reported for the first time, while the remaining three alleles were the same as alleles in other species. A total of 15 nucleotide changes were detected in the 13 alleles, resulting in only 11 amino acid changes because of synonymous mutations. Alleles TaALPb7D-E, TaALPb7D-G, and TaALPb7D-J encoded the same protein. These polymorphic sites existed in the N-terminus, Repetitive region (Left), Repetitive region (Right) and C-terminus domains, with no polymorphisms in the signal peptide sequence nor in those encoding the 18 conserved cysteine residues. Phylogenetic analysis divided the TaALPb7Ds into four clades. The Ae. tauschii alleles were distributed in all four clades, while the alleles derived from common wheat, TaALPb7D-G and TaALPb7D-C, belonged to clade III and IV, respectively. Alleles TaALPb7D-G and TaALPb7D-C were the most widely distributed, being present in nine and six countries, respectively. Iran and Turkey exhibited the highest genetic diversity with respect to TaALPb7D alleles, accessions from these countries carrying seven and six alleles, respectively, which implied that these countries were the centers of origin of the avenin-like b gene. The new alleles discovered and the phylogenetic analysis of avenin-like b genes will provide breeding materials and a theoretical basis for wheat quality improvement.

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Acknowledgements

This research was financially supported by the West Light Foundation of the Chinese Academy of Sciences, the Strategic Priority Research Program of the Chinese Academy of Sciences (XDA08030106), the Ministry of Science and Technology of China (2016YFD0100500), the Science and Technology Planning Project of Qinghai Province, China (2013-Z-942Q and 2017-ZJ-Y14), and the Basic Research Projects of Qinghai Province (2015-ZJ-701).

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  1. Dong Cao and Hongxia Wang have contributed equally to this work.

Authors and Affiliations

  1. Key Laboratory of Adaptation and Evolution of Plateau Biota, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining, Qinghai, 810008, China

    Dong Cao, Hongxia Wang, Bo Zhang, Baolong Liu, Dengcai Liu, Wenjie Chen & Huaigang Zhang

  2. State Key Laboratory Breeding Base-Key Laboratory of Qinghai Province for Plateau Crop Germplasm Innovation and Utilization, Xining, Qinghai, 810008, China

    Wenjie Chen

  3. Key Laboratory of Crop Molecular Breeding of Qinghai Province, Xining, Qinghai, 810008, China

    Dong Cao, Hongxia Wang, Bo Zhang, Baolong Liu, Dengcai Liu, Wenjie Chen & Huaigang Zhang

  4. University of Chinese Academy of Sciences, Beijing, 100049, China

    Dong Cao, Hongxia Wang, Bo Zhang, Baolong Liu, Dengcai Liu, Wenjie Chen & Huaigang Zhang

  5. Triticeae Research Institute, Sichuan Agricultural University, Chengdu, Sichuan, 611130, China

    Dengcai Liu

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  1. Dong Cao
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  2. Hongxia Wang
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  3. Bo Zhang
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Correspondence to Wenjie Chen or Huaigang Zhang.

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Cao, D., Wang, H., Zhang, B. et al. Genetic diversity of avenin-like b genes in Aegilops tauschii Coss. Genetica 146, 45–51 (2018). https://doi.org/10.1007/s10709-017-9995-5

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  • DOI: https://doi.org/10.1007/s10709-017-9995-5

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