Skip to main content
SpringerLink
Log in

Diversity and distribution of puroindoline-D1 genes in Aegilops tauschii

  • Research Article
  • Published:
Genetic Resources and Crop Evolution Aims and scope Submit manuscript

Abstract

Puroindoline-D1 genes are the key genes that decide kernel texture in bread wheat, but limited genetic resources have impeded the improvement of wheat quality. In this study, the puroindoline-D1 genes from 110 accessions of Aegilops tauschii Coss. (the D genomic ancestor of bread wheat) were isolated and sequenced to identify new alleles and discover their genetic diversities. Three new alleles: Pina-D1o, Pinb-D1dt and Pinb-D1it, were discovered, and they were only one nucleotide different to Pina-D1a, Pinb-D1a and Pinb-D1i, respectively. Two new puroindoline proteins were translated from the Pina-D1o and Pinb-D1dt. Pinb-D1it coded the same protein as Pinb-D1o, Pinb-D1m, Pinb-D1h, Pinb-D1n, AJ302108, Pinb-D1i and Pinb-d1it, which are common phenotypes found at the Pina-D1 and Pinb-D1 A. tauschii locus; Pina-D1h, Pina-D1f, Pina-D1e, Pina-D1d, Pina-D1c and AY608595 also coded the same amino acid at the Pina-D1 locus. Total eight Pina-D1 alleles, seven Pinb-D1 alleles and 20 haplotypes were found in these accessions. The synonymous mutations produced two kinds of synonymous haplotypes in our experiment. The first kind contained Pina-D1d/Pinb-D1i, Pina-D1c/Pinb-D1h, Pina-D1c/Pinb-D1i, Pina-D1d/pinb-D1h, Pina-D1j/pinb-D1i and Pina-D1e/Pinb-D1i, and the second kind contained Pina-D1a/Pinb-D1i and Pina-D1a/Pina-D1it. The eight synonymous haplotypes made up the majority of haplotypes, and covered also the majority of the geographical distribution. The large numbers of synonymous haplotypes and their wide distribution suggested that the puroindoline-D1 genes play important roles in A. tauschii survival. New puroindoline alleles and the haplotypes in A. tauschii will improve our understanding of puroindolines evolution and lead to increasing of wheat quality.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4

Similar content being viewed by others

References

  • Ayala M, Guzman C, Alvarez JB, Pena RJ (2013) Characterization of genetic diversity of puroindoline genes in Mexican wheat landraces. Euphytica 190:53–63

    Article  Google Scholar 

  • Biasini M, Bienert S, Waterhouse A, Arnold K, Studer G et al (2014) SWISS-MODEL: modelling protein tertiary and quaternary structure using evolutionary information. Nucleic Acids Res 42:W252–W258

  • Chantret N, Salse J, Sabot F, Rahman S, Bellec A et al (2005) Molecular basis of evolutionary events that shaped the hardness locus in diploid and polyploid wheat species (Triticum and Aegilops). Plant Cell 17:1033–1045

  • Chebotar SV, Kurakina KO, Khokhlov OM, Chebotar GO, Sivolap YM (2012) Phenotypic effects of alleles of the common wheat puroindoline genes. Cytol Genet 46:202–209

    Article  Google Scholar 

  • Chen MJ, Wilkinson M, Tosi P, He G, Shewry P (2005) Novel puroindoline and grain softness protein alleles in Aegilops species with the C, D, S, M and U genomes. Theor Appl Genet 111:1159–1166

  • Chen F, Li HH, Cui DQ (2013) Discovery, distribution and diversity of puroindoline-D1 genes in bread wheat from five countries (Triticum aestivum L.). BMC Plant Biol 13:125

    Article  PubMed  PubMed Central  Google Scholar 

  • Cuesta S, Guzman C, Alvarez JB (2013) Allelic diversity and molecular characterization of puroindoline genes in five diploid species of the Aegilops genus. J Exp Bot 64:5133–5143

  • Edwards MA, Osborne BG, Henry RJ (2010) Puroindoline genotype, starch granule size distribution and milling quality of wheat. J Cereal Sci 52:314–320

    Article  CAS  Google Scholar 

  • Gautier MF, Cosson P, Guirao A, Alary R, Joudrier P (2000) Puroindoline genes are highly conserved in diploid ancestor wheats and related species but absent in tetraploid Triticum species. Plant Sci 153:81–91

  • Gedye KR, Morris CF, Bettge AD (2004) Determination and evaluation of the sequence and textural effects of the puroindoline a and puroindoline b genes in a population of synthetic hexaploid wheat. Theor Appl Genet 109:1597–1603

    Article  CAS  PubMed  Google Scholar 

  • Guzman C, Caballero L, Martin MA, Alvarez JB (2012) Molecular characterization and diversity of the Pina and Pinb genes in cultivated and wild diploid wheat. Mol Breed 30:69–78

    Article  CAS  Google Scholar 

  • Haney EF, Petersen AP, Lau CK, Jing W, Storey DG et al (2013) Mechanism of action of puroindoline derived tryptophan-rich antimicrobial peptides. BBA—Biomembranes 1828:1802–1813

  • Li J, Wan HS, Yang WY (2014) Synthetic hexaploid wheat enhances variation and adaptive evolution of bread wheat in breeding processes. J Syst Evol 52:735–742

    Article  Google Scholar 

  • Luo L, Zhang JR, Yang GX, Li Y, Li K et al (2008) Expression of puroindoline a enhances leaf rust resistance in transgenic tetraploid wheat. Mol Biol Rep 35:195–200

  • Park CS, Kang CS, Cheong YK, Jung W, Woo SH (2010) Influence of puroindoline genotypes on grain characteristics, physico-chemical properties of flour and end-use quality of Korean wheats. Breed Sci 60:233–242

    Article  CAS  Google Scholar 

  • Pauly A, Pareyt B, Fierens E, Delcour JA (2014) Wheat (Triticum aestivum L.) puroindoline functionality in bread making and its impact on bread quality. J Cereal Sci 60:114–121

    Article  CAS  Google Scholar 

  • Rakszegi M, Bognar Z, Li Z, Bekes F, Lang L et al (2010) Effect of milling on the starch properties of winter wheat genotypes. Starch–Starke 62:115–122

  • Reynolds NP, Martin JM, Giroux MJ (2010) Increased wheat grain hardness conferred by novel puroindoline haplotypes from Aegilops tauschii. Crop Sci 50:1718–1727

    Article  CAS  Google Scholar 

  • Tamura K, Dudley J, Nei M, Kumar S (2007) MEGA4: molecular evolutionary genetics analysis (MEGA) software version 4.0. Mol Biol Evol 24:1596–1599

    Article  CAS  PubMed  Google Scholar 

  • Wall ML, Wheeler HL, Smith J, Figeys D, Altosaar I (2010) Mass spectrometric analysis reveals remnants of host-pathogen molecular interactions at the starch granule surface in wheat endosperm. Phytopathology 100:848–854

    Article  CAS  PubMed  Google Scholar 

  • Wang YQ (2014) MeteoInfo: GIS software for meteorological data visualization and analysis. Meteorol Appl 21:360–368

    Article  Google Scholar 

  • Xia LQ, Geng HW, Chen XM, He Z, Lillemo M et al (2008) Silencing of puroindoline a alters the kernel texture in transgenic bread wheat. J Cereal Sci 47:331–338

  • Yan Z, Wan Y, Liu K, Zheng Y, Wang D (2002) Identification of a novel HMW glutenin subunit and comparison of its amino acid sequence with those of homologous subunits. Chin Sci Bull 47:220–225

    Article  Google Scholar 

Download references

Acknowledgments

This research was financially supported by the National Natural Science Foundation of China (Nos. 31071417, 31260322), the West Light Foundation of the Chinese Academy of Sciences, The Projects of Technology Innovation and Function Development of Equipment (lg201307), and the Pilot Projects of Designer Breeding by Molecular Module, Chinese Academy of Sciences.

Author information

Author notes

    Authors and Affiliations

    1. Qinghai Province Key Laboratory of Crop Molecular Breeding, Xining, 810001, Qinghai, China

      Di Liu, Wenjie Chen, Bo Zhang, Dengcai Liu, Baolong Liu & Huaigang Zhang

    2. University of Chinese Academy of Sciences, Beijing, 100049, China

      Di Liu

    3. Key Laboratory of Adaptation and Evolution of Plateau Biota (AEPB), Chinese Academy of Sciences, Xining, 810001, Qinghai, China

      Di Liu, Wenjie Chen, Bo Zhang, Dengcai Liu, Baolong Liu & Huaigang Zhang

    Authors
    1. Di Liu
      View author publications

      You can also search for this author in PubMed Google Scholar

    2. Wenjie Chen
      View author publications

      You can also search for this author in PubMed Google Scholar

    3. Bo Zhang
      View author publications

      You can also search for this author in PubMed Google Scholar

    4. Dengcai Liu
      View author publications

      You can also search for this author in PubMed Google Scholar

    5. Baolong Liu
      View author publications

      You can also search for this author in PubMed Google Scholar

    6. Huaigang Zhang
      View author publications

      You can also search for this author in PubMed Google Scholar

    Corresponding authors

    Correspondence to Baolong Liu or Huaigang Zhang.

    Additional information

    Di Liu and Wenjie Chen have contributed equally to this work.

    Rights and permissions

    Reprints and permissions

    About this article

    Check for updates. Verify currency and authenticity via CrossMark

    Cite this article

    Liu, D., Chen, W., Zhang, B. et al. Diversity and distribution of puroindoline-D1 genes in Aegilops tauschii . Genet Resour Crop Evol 63, 615–625 (2016). https://doi.org/10.1007/s10722-015-0271-1

    Download citation

    • Received:

    • Accepted:

    • Published:

    • Issue Date:

    • DOI: https://doi.org/10.1007/s10722-015-0271-1

    Keywords

    Search

    Navigation