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Genetic and cytogenetic analysis of the American cherry fruit fly, Rhagoletis cingulata (Diptera: Tephritidae)

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Abstract

The American eastern cherry fruit fly, Rhagoletis cingulata, a pest of cherries in the western hemisphere, invaded Europe in 1983, and since then dispersed to several European countries. Information on the genetics and cytogenetics of this pest is very scarce. The mitotic karyotype and detailed photographic maps of the salivary gland polytene chromosomes of R. cingulata are presented here. The mitotic metaphase complement consists of six pairs of chromosomes with the sex chromosomes being very small and similar in size. The analysis of the salivary gland polytene complement shows a total number of five long chromosomes (10 polytene arms), which correspond to the five autosomes of the mitotic nuclei and an extrachromosomal heterochromatic mass, which corresponds to the sex chromosomes. The banding patterns and the most characteristic features and prominent landmarks of each polytene chromosome are presented and discussed. Chromosomal homologies between R. cingulata, R. completa and R. cerasi are also proposed, based on the comparison of chromosome banding patterns. Furthermore, the detection and characterization of Wolbachia pipientis in the R. cingulata population studied is presented and the potential correlation with the asynaptic phenomena found in its polytene complement is discussed. In addition, 10 out of 24 microsatellite markers developed for other Rhagoletis species are cross-amplified, evaluated and proposed as useful markers for population and genetic studies in R. cingulata.

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Acknowledgments

The authors would like to thank Professor Zacharopoulou A (Department of Biology, University of Patras, Greece) for her generous support and encouragement throughout this study. This study was partially supported by IKYDA awarded to N.T. Papadopoulos (University of Thessaly) and H. Vogt (Julius Kühn–Institute).

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  1. Kostas Bourtzis

    Present address: Department of Environmental and Natural Resources, University of Western Greece, 30100, Agrinio, Greece

Authors and Affiliations

  1. Department of Genetics, Development and Molecular Biology, Faculty of Sciences, School of Biology, Aristotle University of Thessaloniki (AUTH), 54124, Thessaloníki, Greece

    Elena Drosopoulou, Ifigeneia Nakou & Penelope Mavragani-Tsipidou

  2. Department of Environmental and Natural Resources, University of Ioannina, 30100, Agrinio, Greece

    Antonios A. Augustinos & Kostas Bourtzis

  3. Centre for Agricultural Technology (LTZ), Nesslerstr. 23-31, 76227, Karlsruhe, Germany

    Kirsten Koeppler

  4. Laboratory of Genes and Development, Department of Biochemistry, University of Oxford, South Parks Road, OX1 3QU, Oxford, UK

    Ilias Kounatidis

  5. Julius Kühn-Institute, Federal Research Centre for Cultivated Plants, Institute for Plant Protection in Fruit Crops and Viticulture, Schwabenheimer Strasse 101, 69221, Dossenheim, Germany

    Heidrun Vogt

  6. Department of Agriculture, Crop Production and Rural Environment, University of Thessaly, Phytokou St., 38446, Ionia (Volos), Magnisia, Greece

    Nikolaos T. Papadopoulos

  7. Biomedical Sciences Research Center Al. Fleming, 16672, Vari, Greece

    Kostas Bourtzis

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  1. Elena Drosopoulou
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Correspondence to Penelope Mavragani-Tsipidou.

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Elena Drosopoulou and Antonios A. Augustinos have contributed equally to this work.

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Drosopoulou, E., Augustinos, A.A., Nakou, I. et al. Genetic and cytogenetic analysis of the American cherry fruit fly, Rhagoletis cingulata (Diptera: Tephritidae). Genetica 139, 1449–1464 (2011). https://doi.org/10.1007/s10709-012-9644-y

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