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Mitotic and polytene chromosomes analysis of the oriental fruit fly, Bactrocera dorsalis (Hendel) (Diptera: Tephritidae)

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Abstract

The Oriental fruit fly, Batrocera dorsalis s.s. (Hendel) is one of the most destructive agricultural pests, belonging to a large group of difficult to distinguish morphologically species, referred as the B. dorsalis complex. We report here a cytogenetic analysis of two laboratory strains of the species and provide a photographic polytene chromosome map from larval salivary glands. The mitotic complement consists of six chromosome pairs including a heteromorphic sex (XX/XY) chromosome pair. Analysis of the polytene complement has shown a total of five polytene chromosomes (10 polytene arms) that correspond to the five autosomes. The most important landmarks of each polytene chromosome and characteristic asynapsis at a specific chromosomal region are presented and discussed. Chromosomal homology between B. dorsalis and Ceratitis capitata has been determined by comparing chromosome banding patterns. The detection of chromosome inversions in both B. dorsalis strains is shown and discussed. Our results show that the polytene maps presented here are suitable for cytogenetic analysis of this species and can be used for comparative studies among species of the Tephritidae family. They also provide a diagnostic tool that could accelerate species identification within the B. dorsalis complex and could shed light on the ongoing speciation in this complex. Polytene chromosome maps can facilitate the development of biological control methods and support the genome mapping project of the species that is currently in progress.

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Acknowledgments

This work forms part of the Joint FAO/IAEA research programme for the development of improved control methodologies against fruit fly pest species. We would also like to thank the two anonymous reviewers for their significant comments on the manuscript.

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  1. Waheed A. A. Sayed

    Present address: Biological Application Department, Nuclear Research Centre, Atomic Energy Authority, Cairo, Egypt

Authors and Affiliations

  1. Joint FAO/IAEA Programme of Nuclear Techniques in Food and Agriculture, International Atomic Energy Agency, Agency’s Laboratories, Seibersdorf, Austria

    Antigone Zacharopoulou, Waheed A. A. Sayed, Alan S. Robinson & Gerald Franz

  2. Department of Biology, University of Patras, Patras, 26500, Greece

    Antigone Zacharopoulou & Antonios A. Augustinos

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  1. Antigone Zacharopoulou
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Zacharopoulou, A., Augustinos, A.A., Sayed, W.A.A. et al. Mitotic and polytene chromosomes analysis of the oriental fruit fly, Bactrocera dorsalis (Hendel) (Diptera: Tephritidae). Genetica 139, 79–90 (2011). https://doi.org/10.1007/s10709-010-9495-3

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