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Spatiotemporal genetic differentiation of Cuban natural populations of the pink shrimp Farfantepenaeus notialis

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Abstract

We analyzed the spatiotemporal genetic structure of Farfantepenaeus notialis populations using five microsatellites loci in order to understand the influence of natural events such as hurricanes on the genetic drift/migration balance as the main cause for the variation of allele frequencies over time. The results were compared with the previous ones obtained from allozymes and mtDNA. High and stable genetic diversity levels (He  =  0.879  ±  0.0015) were found over eight years for the populations that inhabit the south Cuban platform, however significant changes of allele frequencies were detected over time. The F ST estimates, albeit low, revealed significant differences among populations inside the Ana Maria Gulf for 1995 but not for the 1999 and 2003 samples. The F ST, AMOVA and the genetic distance analysis revealed the instability of the genetic structure over time in accordance with allozymes results. The correspondence of the microsatellite results with those obtained from allozymes confirm the effects of migration enhanced by natural events as the main cause of the temporal variation of allele frequencies. The genetic drift effect was discarded through the evaluation of Ne and the M ratio, while natural selection effects were rejected because of the lowest probability of microsatellite loci being under selective pressures. The microsatellite data are also consistent with the results obtained with mtDNA in detecting significant and persistent genetic differences between the Gulfs of Ana María and Batabanó for the years 1995 and 2003.

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Acknowledgements

We thank Nicole Dennebouy for the technical assistance, Ileana Álvarez, Mireya Sosa, Serbilio, Gilma Delgado and Luis Fong from Centro de Investigaciones Pesqueras, for facilitating sampling in commercial vessels and for discussion on fisheries. We thank also the personnel from fishery bureau of Santa Cruz del Sur, Júcaro, Cienfuegos, and Batabanó. We thank the editors and two anonymous referees for reading and providing valuable suggestions to our manuscript. This research was supported by grants from the International Foundation for Science, Stockholm, Sweden to EGM and from the University of Havana, Alma Mater to ARB.

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Authors and Affiliations

  1. Centro de Investigaciones Marinas, Universidad de La Habana, Calle 16, No. 114 entre 1ra y 3ra, Miramar, Playa, Ciudad Habana, 11300, Cuba

    Aymée Robainas-Barcia & Erik García-Machado

  2. Departamento de Biología Funcional, Universidad de Oviedo, Julián Clavería sn, Oveido, 33010, Spain

    Gloria Blanco & José A. Sánchez

  3. Centre de Génétique Moléculaire, CNRS, 91198, Gif-sur-Yvette Cedex, France

    Monique Monnerot

  4. Laboratoire Evolution, Génomes et Spéciation, CNRS, 91198, Gif-sur-Yvette cedex, France

    Michel Solignac

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  1. Aymée Robainas-Barcia
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  2. Gloria Blanco
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  3. José A. Sánchez
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  4. Monique Monnerot
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  5. Michel Solignac
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  6. Erik García-Machado
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Correspondence to Erik García-Machado.

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Robainas-Barcia, A., Blanco, G., Sánchez, J.A. et al. Spatiotemporal genetic differentiation of Cuban natural populations of the pink shrimp Farfantepenaeus notialis . Genetica 133, 283–294 (2008). https://doi.org/10.1007/s10709-007-9212-z

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  • DOI: https://doi.org/10.1007/s10709-007-9212-z

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