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Identification of quantitative trait loci associated with drought tolerance traits in rice (Oryza sativa L.) under PEG and field drought stress

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Two recombinant inbred line F10 rice populations (IAPAR-9/Akihikari and IAPAR-9/Liaoyan241) were used to identify quantitative trait loci (QTLs) for ten drought tolerance traits at the budding and early seedling stage under polyethylene glycol-induced drought stress, and two traits of leaf rolling index (LRI) and leaf withering degree (LWD) under field drought stress. The results showed that the drought-tolerance capacity of IAPAR-9 was stronger than that of Akihikari and Liaoyan241. Thirty-four QTLs for 12 drought tolerance traits were detected, and among them, in the IAPAR-9/Akihikari population, qLRI9-1 and qLRI10-1 for LRI were repeatedly detected in RM3600-RM553 on chromosome 9 and in RM6100-RM3773 on chromosome 10, respectively, at two times points of July 31 and August 13 in 2014. The two QTLs are stable against the environmental impact, and qLRI9-1 and qLRI10-1 explained 6.77–13.66% and 5.01–8.32% of the phenotypic variance, respectively, at the two times points. qLWD9-2 for LWD in the IAPAR-9/Liaoyan241 population contributed 8.73% of variation was detected in the same marker interval with the qLRI9-1, and qLRI1-1 for LRI and qLWD1-1 for LWD were located in the same marker interval RM11054-RM5646 on chromosome 1, which contributed 18.82 and 5.78% of phenotype variation respectively. qGV3 for germination vigor and qRGV3 for relative germination vigor at the budding stage were detected in the same marker interval RM426-RM570 on chromosome 3, which explained 14.98 and 16.30% of the observed phenotypic variation respectively, representing major QTLs. The above-mentioned stable or major QTLs regions could be useful for molecular marker assisted selection breeding, fine mapping, and cloning.

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Acknowledgements

We thank the Chinese National Germplasm Bank for providing the rice seeds. This work was supported by the National Key Research and Development Plan (2016YFD0100101, 2016YFD0100301), the International Cooperation Project from the National Institute of Crop Science, RDA (PJ012113), the National Key Technology Research and Development Program of China (2013BAD01B02-2, 2013BAD01B0101-02, 2015BAD01B01-1), the CAAS Science and Technology Innovation Program, the National Infrastructure for Crop Germplasm Resources (NICGR2016-001), and the Protective Program for Crop Germplasm of China (2016NWB036-01, 2016NWB036-12-2).

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  1. Bing Han, Jiao Wang and Yafei Li contributed equally to this work.

Authors and Affiliations

  1. Key Laboratory of Crop Germplasm Resources and Biotechnology, Ministry of Agriculture, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, the National Key, Facility for Crop Gene Resources and Genetic Improvement, Beijing, 100081, China

    Bing Han, Jiao Wang, Xiaoding Ma, Di Cui, Yanjie Wang, Guilan Cao & Longzhi Han

  2. Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, 100101, China

    Yafei Li

  3. Department of Southern Area Crop Science, National Institute of Crop Science, Rural Development Administration, Milyang, 627-803, South Korea

    Sumin Jo, Dongsoo Park, Youchun Song & Dongjin Shin

  4. Institute of Crop Research, Ningxia Academy of Agricultural Sciences, Yongning, 750105, Ningxia, China

    Xingsheng Wang & Jianchang Sun

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  1. Bing Han
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  2. Jiao Wang
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Correspondence to Jianchang Sun, Dongjin Shin or Longzhi Han.

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Han, B., Wang, J., Li, Y. et al. Identification of quantitative trait loci associated with drought tolerance traits in rice (Oryza sativa L.) under PEG and field drought stress. Euphytica 214, 74 (2018). https://doi.org/10.1007/s10681-018-2138-y

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