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Seed dormancy QTL identification across a Sorghum bicolor segregating population

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An Erratum to this article was published on 23 June 2016

Abstract

Pre-harvest sprouting (PHS) in Sorghum bicolor is one of the main constrains for its production in the central region of Argentina, as grain maturation often coincides with rainy or high environmental humidity conditions. The obtention of more dormant genotypes with higher PHS resistance has always been a desirable trait for breeders but the typical quantitative nature of seed dormancy makes its manipulation difficult through classical breeding. Dissecting this quantitative variability into quantitative trait loci (QTL) is a main concern especially in cereal species. In this work, a sorghum segregating population including 190 families was genotyped with microsatellite markers and the SbABI5 candidate gene. A genetic map encompassing 96 markers and a total length of 1331 cM was built. Seed dormancy was phenotyped in F3 and F4 panicles in two contrasting Argentinean environments (Castelar and Manfredi). Six seed dormancy QTL for mature grains were identified (qGI-1, qGI-3, qGI-4, qGI-6, qGI-7 and qGI-9) with the aid of QTL Cartographer and QTLNetwork, three of them (qGI-3, qGI-7 and qGI-9) being co-localised by both approaches. No epistasis was detected for the identified QTL but QTL-by-environment interaction was significant for qGI-7 and qGI-9. Interestingly, seed dormancy candidate genes SbABI3/VP1 and SbGA20ox3 were located within qGI-3, which makes them noteworthy candidate genes for this QTL.

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Acknowledgments

The authors would like to thank Verónica Lia and Andrea Puebla for their skilful assistance with SSR genotyping and Mirta Tinaro for her qualified technical help with dormancy phenotyping. This work was supported by a grant from the National Agency for Science and Technological Promotion (ANPCyT) PICT 2010 no. 2521 and INTA Project PNBIO 1131042. Renata Cantoro held a PhD grant from the Argentinean National Council of Scientific and Technical Research (CONICET).

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Author notes

  1. Jorge O. Gieco

    Present address: Facultad de Ciencias Agrarias, Universidad Nacional del Litoral, Kreder 2805, S3080HOF, Esperanza, Santa Fe, Argentina

Authors and Affiliations

  1. IFEVA, Universidad de Buenos Aires, CONICET, Facultad de Agronomía, C1417DSE, Buenos Aires, Argentina

    Renata Cantoro, María V. Rodríguez & Roberto L. Benech-Arnold

  2. Instituto de Biotecnología, CICVyA, Instituto Nacional de Tecnología Agropecuaria (INTA)-Castelar, B1686IGC, Hurlingham, Buenos Aires, Argentina

    Luis G. Fernández, Norma Paniego & Ruth A. Heinz

  3. Centro de Estudios Fotosintéticos y Bioquímicos (CEFOBI), Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, S2002LRK, Rosario, Santa Fé, Argentina

    Gerardo D. L. Cervigni

  4. Estación Experimental Agropecuaria Manfredi, Instituto Nacional de Tecnología Agropecuaria (INTA), Manfredi, 5988, Córdoba, Argentina

    Jorge O. Gieco

  5. Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, EHA1428, Ciudad Autónoma de Buenos Aires, Argentina

    Norma Paniego & Ruth A. Heinz

  6. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), C1033AAJ, Ciudad Autónoma De Buenos Aires, Argentina

    Gerardo D. L. Cervigni, María V. Rodríguez, Norma Paniego, Ruth A. Heinz & Roberto L. Benech-Arnold

  7. Cátedra de Cultivos Industriales, Facultad de Agronomía, Universidad de Buenos Aires, C1417DSE, Ciudad Autónoma de Buenos Aires, Argentina

    Roberto L. Benech-Arnold

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  1. Renata Cantoro
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  2. Luis G. Fernández
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Correspondence to Renata Cantoro.

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Ruth A. Heinz and Roberto L. Benech-Arnold have contributed equally to this study.

An erratum to this article can be found at http://dx.doi.org/10.1007/s10681-016-1735-x.

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Cantoro, R., Fernández, L.G., Cervigni, G.D.L. et al. Seed dormancy QTL identification across a Sorghum bicolor segregating population. Euphytica 211, 41–56 (2016). https://doi.org/10.1007/s10681-016-1717-z

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