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Detection of seed dormancy QTL in multiple mapping populations derived from crosses involving novel barley germplasm

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Abstract

Seed dormancy is one of the most important traits in germination process to control malting and pre-harvest sprouting in barley (Hordeum vulgare L.). EST based linkage maps were constructed on seven recombinant inbred (RI) and one doubled haploid (DH) populations derived from crosses including eleven cultivated and one wild barley strains showing the wide range of seed dormancy levels. Seed dormancy of each RI and DH line was estimated from the germination percentage at 5 and 10 weeks post-harvest after-ripening periods in 2003 and 2005. Quantitative trait loci (QTLs) controlling seed dormancy were detected by the composite interval mapping procedure on the RI and DH populations. A total of 38 QTLs clustered around 11 regions were identified on the barley chromosomes except 2H among the eight populations. Several QTL regions detected in the present study were reported on similar positions in the previous QTL studies. The QTL on at the centromeric region of long arm of chromosome 5H was identified in all the RI and DH populations with the different degrees of dormancy depth and period. The responsible gene of the QTL might possess a large allelic variation among the cross combinations, or can be multiple genes located on the same region. The various loci and their different effects in dormancy found in the barley germplasm in the present study enable us to control the practical level of seed dormancy in barley breeding programs.

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References

  • Clerkx EJM, El-Lithy ME, Vierling E, Ruys GJ, de Vries HB, Groot SPC, Vreugdenhil D, Koornneef M (2004) Analysis of natural allelic variation of Arabidopsis seed germination and seed longevity traits between the accessions Landsberg erecta and Shakdara, using a new recombinant inbred line population. Plant Physiol 135:432–443

    Article  PubMed  CAS  Google Scholar 

  • Edney MJ, Mather DE (2004) Quantitative trait loci affecting germination traits and malt friability in a two-rowed by six-rowed barley cross. J Cereal Sci 39:283–290

    Article  CAS  Google Scholar 

  • Foley ME (2001) Seed dormancy: an update on terminology, physiological genetics, and quantitative trait loci regulating germinability. Weed Sci 49:305–317

    Article  CAS  Google Scholar 

  • Frewen BE, Chen TH, Howe GT, Davis J, Rohde A, Boerjan W, Bradshaw HD (2000) Quantitative trait loci and candidate gene mapping of bud set and bud flush in populus. Genetics 154:837–845

    PubMed  CAS  Google Scholar 

  • Gao W, Clancy JA, Han F, Prada D, Kleinhofs A, Ullrich SE (2003) Molecular dissection of a dormancy QTL region near the chromosome 7 (5H) L telomere in barley. Theor Appl Genet 107:552–559

    Article  PubMed  CAS  Google Scholar 

  • Han F, Ullrich SE, Clancy JA, Jitkov V, Kilian A, Romagosa I (1996) Verification of barley seed dormancy loci via linked molecular markers. Theor Appl Genet 92:87–91

    Article  Google Scholar 

  • Han F, Ullrich SE, Clancy JA, Romagosa I (1999) Inheritance and fine mapping of a major barley seed dormancy QTL. Plant Sci 143:113–118

    Article  CAS  Google Scholar 

  • Hori K, Kobayashi T, Shimizu A, Sato K, Takeda K, Kawasaki S (2003) Efficient construction of high-density linkage map and its application to QTL analysis in barley. Theor Appl Genet 107:806–813

    Article  PubMed  CAS  Google Scholar 

  • Hori K, Sato K, Nankaku N, Takeda K (2005) QTL analysis in recombinant chromosome substitution lines and doubled haploid lines derived from a cross between Hordeum vulgare ssp. vulgare and Hordeum vulgare ssp. spontaneum. Mol Breed 16:295–311

    Article  CAS  Google Scholar 

  • Hori K, Sato K, Kobayashi T, Takeda K (2006) QTL analysis of Fusarium head blight severity in recombinant inbred population derived from a cross between two-rowed barley varieties. Breed Sci 56:25–30

    Article  CAS  Google Scholar 

  • Kosambi DD (1944) The estimation of map distances from recombination values. Ann Eugen 12:172–175

    Google Scholar 

  • Lander ES, Green P, Abrahamson J, Barlow A, Daly MJ (1987) MAPMAKER: an interactive computer package for constructing primary genetic linkage maps of experimental and natural populations. Genomics 1:174–181

    Article  PubMed  CAS  Google Scholar 

  • Miura K, Lin SY, Yano M, Nagamine T (2002) Mapping quantitative trait loci controlling seed longevity in rice (Oryza sativa L.). Theor Appl Genet 104:981–986

    Article  PubMed  CAS  Google Scholar 

  • Mori M, Uchino N, Chono M, Kato K, Miura H (2005) Mapping QTL for grain dormancy on wheat chromosome 3A and the group 4 chromosomes, and their combined effect. Theor Appl Genet 110:1315–1323

    Article  PubMed  CAS  Google Scholar 

  • Oberthur L, Blake TK, Dyer WE, Ullrich SE (1995) Genetic analysis of seed dormancy in barley (Hordeum vulgare L.). J Agricultural Genomics 1:5. http://www.cabi-publishing.org/jag/papers95/paper595/indexp595.html

  • Prada D, Ullrich SE, Molina-Cano JL, Cistué L, Clancy JA, Romagosa I (2004) Genetic control of dormancy in a Triumph/Morex cross in barley. Theor Appl Genet 109:62–70

    Article  PubMed  CAS  Google Scholar 

  • Ramsay L, Macaulay M, degli Ivanissevich S, MacLean K, Cardle L, Fuller J, Edwards KJ, Tuvesson S, Morgante M, Massari A, Maestri E, Marmiroli N, Sjakste T, Ganal M, Powell W, Waugh R (2000) A simple sequence repeat-based linkage map of barley. Genetics 156:1997–2005

    PubMed  CAS  Google Scholar 

  • Romagosa I, Han F, Clancy JA, Ullrich SE (1999) Individual locus effects on dormancy during seed development and after ripening in barley. Crop Sci 39:74–79

    Article  Google Scholar 

  • Sato K, Nankaku N, Motoi Y, Takeda K (2004) Large scale mapping of ESTs on barley genome. In: Spunar J, Janikova J (eds) Proceedings of the 9th international barley genetics symposium, vol 1. Brno, pp 79–85

  • Simpson GM (1990) Seed dormancy in grasses. Cambridge University Press, New York

    Google Scholar 

  • Stanca AM, Romagosa I, Takeda K, Lundborg T, Terzi V, Cattivelli L (2003) Diversity in abiotic stress tolerances. In: von Bothmer R, van Hintum Th, Knüpffer H, Sato K (eds) Diversity in barley (Hordeum vulgare). Elsevier, Amsterdam, pp 179–199

    Chapter  Google Scholar 

  • Takeda K (1996) Varietal variarions and inheritance of seed dormancy in barley. In: Proceeding of the 7th international symposium on pre-harvest sprouting in cereals 1995, pp 205–212

  • Takeda K, Hori K (2007) Geographical differentiation and diallel analysis of seed dormancy in barley. Euphytica 153:249–256

    Article  Google Scholar 

  • Ullrich SE, Hayes PM, Dyer WE, Blake TK, Clancy JA (1993) Quantitative trait locus analysis of seed dormancy in ‘Steptoe’ barley. In: Proceedings of the pre-harvest sprouting in cereals 1992. St Paul, pp 136–145

  • Vanhala TK, Stam P (2006) Quantitative trait loci for seed dormancy in wild barley (Hordeum spontaneum C. Koch). Genet Res Crop Evol 53:1013–1019

    Article  CAS  Google Scholar 

  • Wang S, Basten CJ, Zeng ZB (2005) Windows QTL Cartographer version 2.5. Department of Statistics, North Carolina State University, Raleigh, http://statgen.ncsu.edu/qtlcart/HTML/index.html

  • Zheng F, Chen G, Huang Q, Orion O, Krugman T, Fahima T, Korol AB, Nevo E, Gutterman Y (2005) Genetic basis of barley caryopsis dormancy and seedling desiccation tolerance at the germination stage. Theor Appl Genet 110:445–453

    Article  CAS  Google Scholar 

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Acknowledgments

We would like to thank Dr. Patrick M. Hayes for his critical reading of the manuscript and Drs. D. Saisho and M. Sugimoto, Mrs. M. Ishii, H. Ueki and Ms. Y. Motoi for their excellent technical assistance. This research was supported by grant from the Ministry of Agriculture, Forestry and Fisheries of Japan (Integrated research project for plant, insect and animal using genome technology GD-3004).

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Author notes

  1. Kiyosumi Hori

    Present address: National Institute of Agrobiological Sciences, 2-1-2 Kannondai, Tsukuba, 305-8602, Japan

Authors and Affiliations

  1. Research Institute for Bioresources, Okayama University, 2-20-1 Chuo, Kurashiki, 710-0046, Japan

    Kiyosumi Hori, Kazuhiro Sato & Kazuyoshi Takeda

Authors
  1. Kiyosumi Hori
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  2. Kazuhiro Sato
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  3. Kazuyoshi Takeda
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Correspondence to Kazuhiro Sato.

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Communicated by I. Romagosa.

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Hori, K., Sato, K. & Takeda, K. Detection of seed dormancy QTL in multiple mapping populations derived from crosses involving novel barley germplasm. Theor Appl Genet 115, 869–876 (2007). https://doi.org/10.1007/s00122-007-0620-3

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  • DOI: https://doi.org/10.1007/s00122-007-0620-3

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