Skip to main content
SpringerLink
Log in

Mapping of a new gene for resistance to broomrape races higher than F

  • Published:
Euphytica Aims and scope Submit manuscript

Abstract

Broomrape (Orobanche cumana) is one of the most important parasitic plants that can drastically reduce sunflower yield. Breeding for resistance is regarded as the most effective and environmentally friendly control measure. Due to the constant changes in broomrape race composition, i.e. the emergence of races F and higher, the majority of the existing resistance genes have become insufficient for crop protection. In this paper we report the genetic analysis and mapping of a new resistance gene for broomrape races higher than F. Broomrape resistance was evaluated using F1 plants and F2:3 families derived from a cross between the resistant sunflower inbred line AB-VL-8 and the susceptible line L-OS-1. The results showed that F1 plants were susceptible, indicating a recessive resistance. In the derived F2 population and F3 families, the resistance segregation deviated significantly from the one-gene Mendelian ratio. However, marker analysis revealed polymorphism only on LG3, indicating that presumably single gene in this region conferred the resistance. The closest marker to the gene, tentatively designated as or ab-vl-8, was ORS683 with the genetic distance of 1.5 centimorgans. The discovery of or ab-vl-8 will provide a much needed new sunflower resistance gene for new broomrape races, and the associated markers will facilitate the introgression of the gene into different sunflower lines.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3

Similar content being viewed by others

References

  • Acimovic M (1998) Sunflower deseases (In Serbian). Naučni institut za ratarstvo i povrtarstvo, Novi Sad

    Google Scholar 

  • Akhtouch B, Muñoz-Ruz J, Melero-Vara J, Fernández-Martínez J, Domínguez J (2002) Inheritance of resistance to race F of broomrape in sunflower lines of different origins. Plant Breed 121:266–268

    Article  Google Scholar 

  • Alonso LC, Fernández-Escobar V, López G, Rodríguez-Ojeda M, Sallago F (1996) New highly virulent sunflower broomrape (Orobanche cernua Loefl.) pathotype in Spain. In: Advances in parasitic plant research. Proceedings of 6th international symposium on parasitic weeds. Córdoba, pp. 639–644

  • Antonova T (2014) The history of interconnected evolution of Orobanche cumana Wallr. and sunflower in the Russian Federation and Kazakhstan. In: Proceedings of the 3rd symposium on broomrape (Orobanche spp.) in sunflower, Cordoba, 3–6 June 2014, pp. 57–64

  • Batchvarova R (2014) Current situation of sunflower broomrape in Bulgaria. In: Proceedings of the 3rd symposium on broomrape (Orobanche spp.) in sunflower, Cordoba, 3–6 June 2014, pp. 51–54

  • Baum M, Lagudah ES, Appels R (1992) Wide crosses in cereals. Annu Rev Plant Biol 43:117–143

    Article  Google Scholar 

  • Cvejic S, Dedic B, Jocic S, Miladinovic D, Miklic V (2012) Broomrape resistance in newly developed sunflower inbred lines. In: Proceedings of 18th international sunflower conference, Mar del Plata, Feb 27–Mar 1 2012, pp. 1037–1042

  • Dimitrijevic A, Imerovski I, Miladinovic D, Tancic S, Dusanic N, Jocic S, Miklic V (2010) Use of SSR markers in identification of sunflower isogenic lines in late generations of backcrossing. Helia 33:191–198

    Article  Google Scholar 

  • Domínguez J (1996) Estimating effects on yield and other agronomic parameters in sunflower hybrids infested with the new races of sunflower broomrape. In: Proceedings of symposium on disease tolerance in sunflower, Beijing, 12–20 June 1996, pp. 118–123

  • Duca M (2014) Current situation of sunflower broomrape in the Republic of Moldova. In: Proceedings of the 3rd symposium on broomrape (Orobanche spp.) in sunflower, Cordoba, 3–6 June 2014, pp. 44–50

  • Dussle C, Hahn V, Knapp S, Bauer E (2004) Pl Arg from Helianthus argophyllus is unlinked to other known downy mildew resistance genes in sunflower. Theor Appl Genet 109:1083–1086

    Article  CAS  PubMed  Google Scholar 

  • Faure N, Serieys H, Cazaux E, Kaan F, Berville A (2002) Partial hybridization in wide crosses between cultivated sunflower and the perennial Helianthus species H. mollis and H. orgyalis. Ann Bot 89:31–39

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Fernandez-Martinez J, Perez-Vich B, Akhtouch B, Velasco L, Munoz-Ruz J, Malero-Vara JM, Dominguez J (2004) Registration of four sunflower germplasms resistant to race F of broomrape. Crop Sci 44:1033–1034

    Article  Google Scholar 

  • Fernández-Martínez JM, Domínguez J, Pérez-Vich B, Velasco L (2008) Update on breeding for resistance to sunflower broomrape. Helia 31:73–84

    Article  Google Scholar 

  • Gentzbittel L, Vear F, Zhang YX, Bervillé A, Nicolas P (1995) Development of a consensus linkage RFLP map of cultivated sunflower (Helianthus annuus L.). Theor Appl Genet 90:1079–1086

    Article  CAS  PubMed  Google Scholar 

  • Gong L, Hulke BS, Gulya TJ, Markell SG, Qi LL (2013) Molecular tagging of a novel rust resistance gene R 12 in sunflower (Helianthus annuus L.). Theor Appl Genet 126:93–99

    Article  CAS  PubMed  Google Scholar 

  • Gressel J, Joel DM (2013) Weedy Orobanchaceae: the problem. In: Joel DM, Gressel J, Musselman LJ (eds) Parasitic Orobanchaceae. Springer, Berlin/Heidelberg, pp 309–312

    Chapter  Google Scholar 

  • Hackett CA, Broadfoot LB (2003) Effects of genotyping errors, missing values and segregation distortion in molecular marker data on the construction of linkage maps. Heredity 90:33–38

    Article  CAS  PubMed  Google Scholar 

  • He X, Liu X, Wang L, Wang L, Lin F, Cheng Y, Chen Z, Liao Y, Pan Q (2012) Identification of the novel recessive gene pi55 (t) conferring resistance to Magnaporthe oryzae. Sci China Life Sci 55:141–149

    Article  CAS  PubMed  Google Scholar 

  • Imerovski I, Dimitrijevic A, Miladinovic D, Dedic B, Jocic S, Miklic V (2012) Towards Orobanche resistance in sunflower—SSR analysis of novel resistance source. In: Proceedings of 18th international sunflower conference, Mar del Plata, Feb 27–Mar 1 2012, pp. 1043–1047

  • Imerovski I, Dimitrijevic A, Miladinovic D, Dedic B, Jocic S, Kovacevic B, Obreht D (2013) Identification of PCR markers linked to different Or genes in sunflower. Plant Breed 132:115–120

    Article  CAS  Google Scholar 

  • Iuoras M, Stanciu D, Ciucã M, Nãstase D, Geronzi F (2004) Preliminary studies related to the use of marker assisted selection for resistance to Orobanche cumana Wallr. in sunflower. Rom Agric Res 21:33–37

    Google Scholar 

  • Iyer-Pascuzzi AS, McCouch SR (2007) Recessive resistance genes and the Oryza sativa-Xanthomonas oryzae pv. oryzae pathosystem. Mol Plant Microbe Interact 20:731–739

    Article  CAS  PubMed  Google Scholar 

  • Jan CC, Fernandez-Martinez J, Ruso J, Munoz-Ruz J (2002) Registration of four sunflower germplasm with resistance to Orobanche cumana race F. Crop Sci 42:2217–2218

    Article  Google Scholar 

  • Jocic S, Miladinovic D, Imerovski I, Dimitrijević A, Cvejic S, Nagl N, Kondic-Spika A (2012) Towards sustainable downy mildew resistance in sunflower—a review. Helia 35:61–72

    Article  Google Scholar 

  • Kaya Y, Evci G, Pekcan V, Gucer T (2004) Determining new broomrape-infested areas, resistant lines and hybrids in Trakya region of Turkey. Helia 27:211–218

    Article  Google Scholar 

  • Kaya Y, Jocic S, Miladinovic D (2012) Sunflower. In: Gupta SK (ed) Technological innovations in major world oil crops: Breeding. Springer, Dordrecht/Heidelberg, pp 85–129

    Chapter  Google Scholar 

  • Kosambi D (1943) The estimation of map distances from recombination values. Ann Eugen 12:172–175

    Article  Google Scholar 

  • Lenardon SL, Bazzalo ME, Abratti G, Cimmino CJ, Galella MT, Grondona M, Giolitti F, León AJ (2005) Screening sunflower for resistance to sunflower chlorotic mottle virus and mapping the Rcmo-1 resistance gene. Crop Sci 45:735–739

    Article  CAS  Google Scholar 

  • Lu YH, Gagne G, Grezes-Besset B, Blanchard P (1999) Integration of a molecular linkage group containing the broomrape resistance gene Or5 into an RFLP map in sunflower. Genome 42:453–456

    Article  CAS  Google Scholar 

  • Lu Y, Melero-Vara J, García-Tejada J, Blanchard P (2000) Development of SCAR markers linked to the gene Or5 conferring resistance to broomrape (Orobanche cumana Wallr.) in sunflower. Theor Appl Genet 100:625–632

    Article  CAS  Google Scholar 

  • Malidža G, Jocić S, Škorić D (2003) Weed and broomrape (Orobanche cernua). control in Cliearfield sunflower, Proceedings of 7th EWRS Mediterranean symposium, Adana, 6–9 May 2003, pp. 51–53

  • Michelmore RW, Paran I, Kesseli R (1991) Identification of markers linked to disease-resistance genes by bulked segregant analysis: a rapid method to detect markers in specific genomic regions by using segregating populations. Proc Nat Acad Sci USA 88:9828–9832

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Miladinovic D, Dedic B, Quiróz F, Alvarez D, Poverene M, Cantamutto M (2012) Orobanche cumana wallr. resistance of commercial sunflower cultivars grown in Argentina. J Basic Appl Genet 23:37–41

    Google Scholar 

  • Molinero-Ruiz L, García-Carneros AB, Collado-Romero M, Raranciuc S, Domínguez J, Melero-Vara JM (2014) Pathogenic and molecular diversity in highly virulent populations of the parasitic weed Orobanche cumana (sunflower broomrape) from Europe. Weed Res 54:87–96

    Article  Google Scholar 

  • Montesclaros L, Nicol N, Ubalijoro E, Leclerc-Potvin C, Ganivet L, Laliberté J-F, Fortin M (1997) Response to potyvirus infection and genetic mapping of resistance loci to potyvirus infection in Lactuca. Theor Appl Genet 94:941–946

    Article  CAS  Google Scholar 

  • Pacureanu-Joita M (2014) Current situation of sunflower broomrape (Orobanche cumana Wallr.) in Romania. In: Proceedings of the 3rd international symposium on broomrape (Orobanche spp.) in sunflower, Cordoba, 3–6 June 2014, pp. 39–43

  • Pacureanu-Joita M, Vranceanu A, Soare G, Marinescu A, Sandu I (1998) The evaluation of the parasite-host interaction system (Helianthus annuus L.)-(Orobanche cumana Wallr.) in Romania. In: Proceedings of 2nd Balkan symposium on field crops, Novi Sad, 16–20 June 1998, pp. 16–20

  • Pacureanu-Joita M, Raranciuc S, Stanciu D, Sava E, Nastase D (2008) Virulence and aggressiveness of sunflower broomrape (Orobanche cumana Wallr.) populations in Romania. Rom Agric Res 25:47–51

    Google Scholar 

  • Panchenko A (1975) Early diagnosis of broomrape resistance in breeding and improving seed production of sunflower. Vestnik-SHN 2(2):107–115

    Google Scholar 

  • Pavan S, Jacobsen E, Visser RG, Bai Y (2010) Loss of susceptibility as a novel breeding strategy for durable and broad-spectrum resistance. Mol Breed 25:1–12

    Article  PubMed  PubMed Central  Google Scholar 

  • Pavan S, Schiavulli A, Appiano M, Marcotrigiano AR, Cillo F, Visser RG, Bai Y, Lotti C, Ricciardi L (2011) Pea powdery mildew er1 resistance is associated to loss-of-function mutations at a MLO homologous locus. Theor Appl Genet 123:1425–1431

    Article  PubMed  Google Scholar 

  • Pavan S, Schiavulli A, Appiano M, Miacola C, Visser RG, Bai Y, Lotti C, Ricciardi L (2013) Identification of a complete set of functional markers for the selection of er1 powdery mildew resistance in Pisum sativum L. Mol Breed 31(1):247–253

    Article  CAS  Google Scholar 

  • Perez-De-Luque A, Eizenberg H, Grenz JH, Sillero JC, Avila C, Sauerborn J, Rubiales D (2010) Broomrape management in faba bean. Field Crops Res 115:319–328

    Article  Google Scholar 

  • Pérez-Vich B, Velasco L, Rich PJ, Ejeta G (2013) Marker-assisted and physiology-based breeding for resistance to root parasitic Orobanchaceae. In: Joel DM, Gressel J, Musselman LJ (eds) Parasitic Orobanchaceae. Springer, Berlin/Heidelberg, pp 369–391

    Chapter  Google Scholar 

  • Permingeat HR, Romagnoli MV, Vallejos RH (1998) A simple method for isolating high yield and quality DNA from cotton (Gossypium hirsutum L.) leaves. Plant Mol Biol Rep 16:1–6

    Article  Google Scholar 

  • Pototskyi G (2014) Current situation of sunflower broomrape in Ukraine. In: Proceedings of the 3rd symposium on broomrape (Orobanche spp.) in sunflower, Cordoba, 3–6 June 2014

  • Qi LL, Long YM, Jan CC, Ma GJ, Gulya TJ (2015) Pl 17 is a novel gene independent of known downy mildew resistance genes in the cultivated sunflower (Helianthus annuus L.). Theor Appl Genet 128:757–767

    Article  CAS  PubMed  Google Scholar 

  • Quillet MC, Madjidian N, Griveau Y, Serieys H, Tersac M, Lorieux M, Bervillé A (1995) Mapping genetic factors controlling pollen viability in an interspecific cross in Helianthus sect. Helianthus. Theor Appl Genet 9:1195–1202

    Google Scholar 

  • Rieseberg LH, Ellstrand N, Arnold M (1993) What can molecular and morphological markers tell us about plant hybridization? Crit Rev Plant Sci 12:213–241

    CAS  Google Scholar 

  • Rieseberg LH, Sinervo B, Linder CR, Ungerer MC, Arias OM (1996) Role of gene interactions in hyhrid speciatjon: evidence from ancient and experimental hybrids. Science 272:741–745

    Article  CAS  PubMed  Google Scholar 

  • Rieseberg LH, Baird S, Gardner K (2000) Hybridization, introgression, and linkage evolution. Plant Mol Biol 42:205–224

    Article  CAS  PubMed  Google Scholar 

  • Rodríguez-Ojeda M, Fernández-Escobar J, Alonso L (2001) Sunflower inbred line (KI-374) carrying two recessive genes for resistance against a highly virulent Spanish population of Orobanche cernua Loefl O cumana. In: Proceedings of the 7th international parasitic weed symposium, Nantes, 5–8 June 2001, pp. 208–211

  • Román B (2013) Population diversity and dynamics of parasitic weeds. In: Joel DM, Gressel J, Musselman LJ (eds) Parasitic Orobanchaceae. Springer, Berlin/Heidelberg, pp 345–356

    Chapter  Google Scholar 

  • Rubiales D et al (2006) Screening techniques and sources of resistance against parasitic weeds in grain legumes. Euphytica 147:187–199

    Article  Google Scholar 

  • Sambrook J, Russell DW (2001) Molecular Cloning: A laboratory manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York

    Google Scholar 

  • Sharma HC (1995) How wide can a wide cross be? Euphytica 82:43–64

    Article  Google Scholar 

  • Shi BX, Chen GH, Zhang ZJ, Hao JJ, Jing L, Zhou HY, Zhao J (2015) First report of race composition and distribution of sunflower broomrape, Orobanche cumana, in China. Plant Dis 99:291

    Article  Google Scholar 

  • Skoric D, Jocic S (2005) Broomrape (Orobanche cumana Wallr.) and its possible control by genetic and chemical means. In: Proceedings of the 46th oil industry conference, Petrovac na moru, 6–10 June 2005, pp. 9–21 (In Serbian)

  • Skoric D, Pacureanu M (2010) Sunflower breeding for resistance to broomrape (Orobanche cumana Wallr.). In: Proceedings of the international symposium “Sunflower breeding on resistance to diseases”. Krasnodar, pp. 19–30

  • Tang S, Heesacker A, Kishore VK, Fernandez A, Sadik ES, Cole G, Knapp SJ (2003) Genetic mapping of the gene for resistance to race E in sunflower. Crop Sci 43:1021–1028

    Article  CAS  Google Scholar 

  • Velasco L, Pérez-Vich B, Jan C, Fernández-Martínez J (2007) Inheritance of resistance to broomrape (Orobanche cumana Wallr.) race F in a sunflower line derived from wild sunflower species. Plant Breed 126:67–71

    Article  Google Scholar 

  • Velasco L, Pérez-Vich B, Yassein AA, Jan CC, Fernández-Martínez JM (2012) Inheritance of resistance to sunflower broomrape (Orobanche cumana Wallr.) in an interspecific cross between Helianthus annuus and Helianthus debilis subsp. tardiflorus. Plant Breed 131:220–221

    Article  Google Scholar 

  • Vrânceanu A, Tudor V, Stoenescu F, Pirvu N (1980) Virulence groups of Orobanche cumana Wallr. differential hosts and resistance sources and genes in sunflower. In: Proceedings of the 9th international sunflower conference, Torremolinos, 8–13 June 1980, pp 74–82

  • Yu J-K et al (2003) Towards a saturated molecular genetic linkage map for cultivated sunflower. Crop Sci 43:367–387

    Article  CAS  Google Scholar 

Download references

Acknowledgments

The authors are grateful to Zvonimir Sakac MSc for technical assistance in the preparation of the manuscript. This work was supported by the Ministry of Education, Science and Technological Development, Republic of Serbia, Project TR 31025.

Author information

Authors and Affiliations

  1. Institute of Field and Vegetable Crops, Maksima Gorkog 30, 21000, Novi Sad, Serbia

    Ivana Imerovski, Aleksandra Dimitrijević, Dragana Miladinović, Boško Dedić, Siniša Jocić & Sandra Cvejić

  2. Faculty of Sciences, University of Novi Sad, Trg Dositeja Obradovica 2, 21000, Novi Sad, Serbia

    Natasa Kočiš Tubić

Authors
  1. Ivana Imerovski
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Aleksandra Dimitrijević
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Dragana Miladinović
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Boško Dedić
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Siniša Jocić
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Natasa Kočiš Tubić
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Sandra Cvejić
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Dragana Miladinović.

Ethics declarations

Conflict of interest

The authors declare that they have no conflict of interest.

Electronic supplementary material

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Imerovski, I., Dimitrijević, A., Miladinović, D. et al. Mapping of a new gene for resistance to broomrape races higher than F. Euphytica 209, 281–289 (2016). https://doi.org/10.1007/s10681-015-1597-7

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10681-015-1597-7

Keywords

Search

Navigation