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Pea powdery mildew er1 resistance is associated to loss-of-function mutations at a MLO homologous locus

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Abstract

The powdery mildew disease affects several crop species and is also one of the major threats for pea (Pisum sativum L.) cultivation all over the world. The recessive gene er1, first described over 60 years ago, is well known in pea breeding, as it still maintains its efficiency as a powdery mildew resistance source. Genetic and phytopathological features of er1 resistance are similar to those of barley, Arabidopsis, and tomato mlo powdery mildew resistance, which is caused by the loss of function of specific members of the MLO gene family. Here, we describe the obtainment of a novel er1 resistant line by experimental mutagenesis with the alkylating agent diethyl sulfate. This line was found to carry a single nucleotide polymorphism in the PsMLO1 gene sequence, predicted to result in premature termination of translation and a non-functional protein. A cleaved amplified polymorphic sequence (CAPS) marker was developed on the mutation site and shown to be fully co-segregating with resistance in F2 individuals. Sequencing of PsMLO1 from three powdery mildew resistant cultivars also revealed the presence of loss-of-function mutations. Taken together, results reported in this study strongly indicate the identity between er1 and mlo resistances and are expected to be of great breeding importance for the development of resistant cultivars via marker-assisted selection.

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Authors and Affiliations

  1. Department of Agroforestry, Environmental Biology and Chemistry, Section of Genetics and Plant Breeding, University of Bari, Via Amendola 165/A, 70126, Bari, Italy

    Stefano Pavan, Michela Appiano, Angelo R. Marcotrigiano & Luigi Ricciardi

  2. Graduate School Experimental Plant Sciences, Droevendaalsesteeg 1, 6708 PB, Wageningen, The Netherlands

    Stefano Pavan

  3. Department of Agro-Environmental Science, Chemistry and Crop Protection, University of Foggia, Via Napoli 25, 71100, Foggia, Italy

    Adalgisa Schiavulli & Concetta Lotti

  4. National Research Council, Institute of Plant Virology, Via Amendola 165/A, 70126, Bari, Italy

    Fabrizio Cillo

  5. Laboratory of Plant Breeding, Wageningen University, Droevendaalsesteeg 1, 6708 PB, Wageningen, The Netherlands

    Richard G. F. Visser & Yuling Bai

Authors
  1. Stefano Pavan
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  2. Adalgisa Schiavulli
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  3. Michela Appiano
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  4. Angelo R. Marcotrigiano
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  5. Fabrizio Cillo
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  6. Richard G. F. Visser
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  7. Yuling Bai
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  8. Concetta Lotti
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  9. Luigi Ricciardi
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Correspondence to Stefano Pavan.

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Communicated by B. Diers.

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Pavan, S., Schiavulli, A., Appiano, M. et al. Pea powdery mildew er1 resistance is associated to loss-of-function mutations at a MLO homologous locus. Theor Appl Genet 123, 1425–1431 (2011). https://doi.org/10.1007/s00122-011-1677-6

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  • DOI: https://doi.org/10.1007/s00122-011-1677-6

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