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Mapping of QTL associated with waterlogging tolerance and drought resistance during the seedling stage in oilseed rape (Brassica napus)

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Abstract

Soil waterlogging and drought are major environmental stresses that suppress rapeseed (Brassica napus) growth and yield. To identify quantitative trait loci (QTL) associated with waterlogging tolerance and drought resistance at the rapeseed seedling stage, we generated a doubled haploid (DH) population consisting of 150 DH lines from a cross between two B. napus lines, namely, line No2127-17 × 275B F4 (waterlogging-tolerant and drought-resistant) and line Huyou15 × 5900 F4 (waterlogging-sensitive and drought-sensitive). A genetic linkage map was constructed using 183 simple sequence repeat and 157 amplified fragment length polymorphism markers for the DH population. Phenotypic data were collected under waterlogging, drought and control conditions, respectively, in two experiments. Five traits (plant height, root length, shoot dry weight, root dry weight and total dry weight) were investigated. QTL associated with the five traits, waterlogging tolerance coefficient (WTC) and drought resistance coefficient (DRC) of all the traits were identified via composite interval mapping, respectively. A total of 28 QTL were resolved for the five traits under control conditions, 26 QTL for the traits under waterlogging stresses and 31 QTL for the traits under drought conditions. Eleven QTL were detected by the WTC, and 19 QTL related to DRC were identified. The results suggest that the genetic bases of both waterlogging tolerance and drought resistance are complex. Some of the QTL for waterlogging tolerance-related traits overlapped with QTL for drought resistance-related traits, indicating that the genetic bases of waterlogging tolerance and drought resistance in the DH population were related in some degree.

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Acknowledgments

This research was financed by the funds from the High-tech program “863” (2006AA10Z146), the High-tech program “863” (2006AA10A), the National Key Basic Research Special Foundation of China (2001CB1088), the Program for Changjiang Scholar and Innovative Research Team in university (IRT0442), and the Program of “948” (2003-Q04).

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Authors and Affiliations

  1. National Key Laboratory of Crop Genetic Improvement, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, 430070, China

    Tingdong Fu, Guangsheng Zhou & Jinxing Tu

  2. Department of Agriculture and Bioengineering, Jinhua College of Profession and Technology, Jinhua, 321007, China

    Zhen Li, Shufang Mei, Zhong Mei & Xianglei Liu

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  1. Zhen Li
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  2. Shufang Mei
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  3. Zhong Mei
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  4. Xianglei Liu
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  5. Tingdong Fu
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  6. Guangsheng Zhou
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  7. Jinxing Tu
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Correspondence to Guangsheng Zhou.

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Li, Z., Mei, S., Mei, Z. et al. Mapping of QTL associated with waterlogging tolerance and drought resistance during the seedling stage in oilseed rape (Brassica napus). Euphytica 197, 341–353 (2014). https://doi.org/10.1007/s10681-014-1070-z

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