Abstract
Genetic study on important traits of tea is difficult because of its self-incompatibility in nature. Moreover, development of a new variety usually needs more than 20 years, since it takes many years from seedling to matured plants for trait investigation. Genetic map is an essential tool for genetic study and breeding. In this study, we have developed an integrated genetic map of tea (Camellia sinensis) using a segregating F1 population derived from a cross between two commercial cultivars (‘TTES 19’ and ‘TTES 8’). A total of 574 polymorphic markers (including SSR, CAPS, STS, AFLP, ISSR and RAPD), 69 markers with highly significant levels of segregation distortion (P < 0.001) (12.0 %) were excluded from further analyses. Of the 505 mapped markers, there were 265 paternal markers (52.5 %), 163 maternal markers (32.3 %), 65 doubly heterozygous dominant markers (12.9 %), and 12 co-dominant markers (2.4 %). The co-dominant markers and doubly heterozygous dominant markers were used as bridge loci for the integration of the paternal and maternal maps. The integrated map comprised 367 linked markers, including 36 SSR, 3 CAPS, 1 STS, 250 AFLP, 13 ISSR and 64 RAPD that were assigned to 18 linkage groups. The linkage groups represented a total map length of 4482.9 cM with a map density of 12.2 cM. This genetic map has the highest genetic coverage so far, which could be applied to comparative mapping, QTL mapping and marker assisted selection in the future.
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Abbreviations
- MAS:
-
Marker-assisted selection
- QTL:
-
Quantitative trait loci
- SSR:
-
Simple sequence repeats or microsatellites
- CAPS:
-
Cleaved amplified polymorphic sequence
- PCR-RFLP:
-
Polymerase chain reaction-restriction fragment length polymorphism
- STS:
-
Sequence tagged site
- EST:
-
Expressed sequence tag
- LG:
-
Linkage groups
- cM:
-
centiMorgan
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Acknowledgments
The authors would like to thank Dr. Kai-Yi Chen of the Department of Agronomy, National Taiwan University in Taiwan for his kind assistance in providing us with the software JoinMap® v4.1.
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Hu, CY., Lee, TC., Tsai, HT. et al. Construction of an integrated genetic map based on maternal and paternal lineages of tea (Camellia sinensis). Euphytica 191, 141–152 (2013). https://doi.org/10.1007/s10681-013-0908-0
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DOI: https://doi.org/10.1007/s10681-013-0908-0