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Isolation and characterization of a stress responsive small GTP-binding protein AhRabG3b in peanut (Arachis hypogaea L.)

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Abstract

Rab7 is a member of small GTP-binding protein family involved in intracellular vesicle trafficking from late endosome to vacuole, some of them are found to be responsive by different environmental stresses. Here we report the isolation of a Rab7 (RabG3b) cDNA from peanut (Arachis hypogaea). Overexpression of the open reading frame (ORF) of Rab7 cDNA encoding 205 amino acids in E. coli conferred the cells tolerance to various abiotic stresses. Transcription of AhRabG3b was differentially up-regulated by different environmental stimuli such as cold, dehydration, NaCl and the plant hormone ABA. Overexpression of the AhRabG3b exhibited enhanced tolerance to drought and salinity stresses in transgenic peanut. These results suggested that the AhRabG3b be a potential candidate gene responsible for both salinity and dehydration tolerance in cultivated peanut.

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Acknowledgments

We thank Professor Chunhai Dong for assistance in English language editing. This study was financially supported by the National Natural Science Foundation of P.R. China (30871544) and the Youth Foundation of Natural Science Foundation of Shandong province (ZR2011CQ026).

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Authors and Affiliations

  1. College of Life Science, , Qingdao Agricultural University/Key Lab of Plant Biotechnology in Universities of Shandong, Qingdao, 266109, China

    Jiong-Ming Sui, Rui Li, Qian-Cheng Fan, Chun-Hua Zheng, Jing-Shan Wang & Li-Xian Qiao

  2. Department of Biology, Lund University, Solvegatan 37, 223 62, Lund, Sweden

    Lin Song

  3. Peanut Research Institute, Shandong Academy of Agricultural Sciences, Qingdao, 266100, China

    Shan-Lin Yu

Authors
  1. Jiong-Ming Sui
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  2. Rui Li
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  3. Qian-Cheng Fan
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  4. Lin Song
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  5. Chun-Hua Zheng
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  6. Jing-Shan Wang
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  7. Li-Xian Qiao
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  8. Shan-Lin Yu
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Corresponding author

Correspondence to Shan-Lin Yu.

Additional information

J.-M. Sui, R. Li and Q.-C. Fan contributed equally to this study.

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Sui, JM., Li, R., Fan, QC. et al. Isolation and characterization of a stress responsive small GTP-binding protein AhRabG3b in peanut (Arachis hypogaea L.). Euphytica 189, 161–172 (2013). https://doi.org/10.1007/s10681-012-0739-4

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  • DOI: https://doi.org/10.1007/s10681-012-0739-4

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