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RETRACTED ARTICLE: A reexamination of molecular markers for use in marker-assisted breeding in tomato

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This article was retracted on 24 July 2013

Abstract

Molecular markers have been used for identification and mapping of genes and QTLs for numerous agriculturally important traits in tomato, including resistance/tolerance to biotic and abiotic stresses and fruit- and flower-related characteristics. However, the extent to which markers have been utilized in tomato breeding programs has not been clearly determined. A review of the literature indicated that the utility of most markers for use in tomato breeding programs have not been verified. Many markers are not validated across tomato genotypes or are not polymorphic within tomato breeding populations. In this study, we examined the utility of available markers for several major disease resistance traits in tomato by testing them in a number of breeding lines and commercial cultivars with known resistance/susceptibility responses. While several markers were validated, others needed PCR optimization for successful amplifications or were not informative in the genotypes used. Specifically, of the 37 markers examined 19 (~51%) were informative, including markers for resistance to Fusarium wilt, late blight, bacterial wilt, tomato mosaic virus, tomato spotted wilt virus, and root knot nematodes. It appears that many of the available markers may need to be further refined or examined for trait association and presence of polymorphism in breeding lines and populations. However, with recent advances in tomato sequencing, it is becoming increasingly possible to develop more informative markers to accelerate the use of MAS in tomato breeding.

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Acknowledgments

We would like to thank Professors John Williamson, Bryon Sosinski and Allan Brown, Drs. Matthew Kinkade and Heather Merk, and graduate students Matthew Sullenberger, Erik Ohlson and Yang Bian for reviewing the manuscript before submission and helpful suggestions. Technical help of Ragy Ibrahem is highly appreciated.

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Authors and Affiliations

  1. Department of Horticultural Science, North Carolina State University, Mountain Horticultural Crops Research and Extension Center, Mills River, NC, 28759, USA

    Dilip R. Panthee

  2. Department of Horticulture and The Intercollege Graduate Degree Programs in Plant Biology and Genetics, The Pennsylvania State University, University Park, PA, 16802, USA

    Majid R. Foolad

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  1. Dilip R. Panthee
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Correspondence to Majid R. Foolad.

Additional information

A retraction note to this article is available at http://dx.doi.org/10.1007/s10681-013-0966-3.

This article has been retracted because large parts of this paper are similar or identical to those first published by the same authors as: Majid R. Foolad & Dilip R. Panthee (2012) Marker-Assisted Selection in Tomato Breeding. Critical Reviews in Plant Sciences 31:93–123.

An erratum to this article is available at http://dx.doi.org/10.1007/s10681-013-0966-3.

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Panthee, D.R., Foolad, M.R. RETRACTED ARTICLE: A reexamination of molecular markers for use in marker-assisted breeding in tomato. Euphytica 184, 165–179 (2012). https://doi.org/10.1007/s10681-011-0544-5

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