Abstract
The best levels of broad-spectrum Tospovirus resistance reported in tomatoes thus far are conferred by the Sw-5 locus. This locus contains at least five paralogues (denoted Sw-5a through Sw-5e), of which Sw-5b represents the actual resistance gene. Here we evaluated a panel of seven PCR primer pairs matching different sequences within a genomic region spanning the Sw-5a and Sw-5b gene cluster. Primer efficiency evaluation was done employing tomato isolines with and without the Sw-5 locus. One primer pair produced a single and co-dominant polymorphism between susceptible and resistant isolines. Sequence analysis of these amplicons indicated that they were specific for the Sw-5 locus and their differences were due to insertions/deletions. The polymorphic SCAR amplicon encompass a conserved sequence of the promoter region of the functional Sw-5b gene, being located in the position −31 from its open reading frame. This primer pair was also evaluated in field assays and with a collection of accessions known to be either susceptible or resistant to tospoviruses. An almost complete correlation was found between resistance under greenhouse/field conditions and the presence of the marker. Therefore, this primer pair is a very useful tool in marker-assisted selection systems in a large range of tomato accessions.
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Acknowledgments
This work was supported by Embrapa Horticultural Crops (CNPH), University of Brasília and Wageningen University and was financed by CAPES (Coordenação de Aperfeiçoamento de Pessoal de Nível Superior) and CNPq (Conselho Nacional de Desenvolvimento Científico e Tecnológico). We would like to thank the technical staff, especially Antonio Francisco Costa, Oneilson Medeiros de Aquino, Lúcio Flávio Barbosa, William Pereira Dutra, and Dick Lohuis.
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An erratum to this article can be found at http://dx.doi.org/10.1007/s11032-009-9329-0
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Dianese, É.C., de Fonseca, M.E.N., Goldbach, R. et al. Development of a locus-specific, co-dominant SCAR marker for assisted-selection of the Sw-5 (Tospovirus resistance) gene cluster in a wide range of tomato accessions. Mol Breeding 25, 133–142 (2010). https://doi.org/10.1007/s11032-009-9313-8
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DOI: https://doi.org/10.1007/s11032-009-9313-8