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QTL mapping of bio-energy related traits in Sorghum

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Abstract

Plant height (PHT), stem and leaf fresh weight (SLFW), juice weight (JW) and sugar content of stem (Brix) are important traits for biofuel production in sweet Sorghum. QTL analysis of PHT, SLFW, JW and Brix was conducted with composite interval mapping using F2 and F2:3 populations derived from the cross between grain Sorghum (Shihong137) × sweet Sorghum (L-Tian). Three QTLs controlling PHT were mapped on SBI-01, SBI-07 and SBI-09 under four different environments. These QTLs could explain 10.16 to 45.29% of the phenotypic variance. Two major effect QTLs on SBI-07 and SBI-09 were consistently detected under four environments. Eight QTLs controlling SLFW were mapped across three environments and accounted for 5.49–25.36% of the phenotypic variance. One major QTL on SBI-09 located between marker Sb5-206 and SbAGE03 was observed under three environments. Four QTLs controlling Brix were identified under two environments and accounted for 11.03–17.65% of the phenotypic variance. Six QTLs controlling JW were detected under two environments, and explained 6.63–23.56% of the phenotypic variance. QTLs for JW on SBI-07 and SBI-09 were consistent in two environments showing higher environmental stability. In addition, two chromosome regions on SBI-07 and SBI-09 were identified in our study having major effect on PHT, SFLW and JW. The results would be useful for the genetic improvement of sweet Sorghum to be used for biofuel production.

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Reference

  • Bhattramakki D, Dong J, Chhabra AK, Hart G (2000) An integrated SSR and RFLP linkage map of Sorghum bicolor (L.) Moench. Genome 43:988–1002

    PubMed  CAS  Google Scholar 

  • Bian YL, Yazaki SJ, Inoue M, Cai HW (2006) QTLs for sugar content of stalk in sweet Sorghum (Sorghum bicolor L. Moench). Agric Sci China 5:736–744

    Article  CAS  Google Scholar 

  • Brown SM, Hopkins MS, Mitchell SE (1996) Multiple methods for the identification of polymorphic simple sequence repeats (SSRs) in Sorghum (Sorghum bicolor (L.) Moench). Theor Appl Genet 93:190–198

    Article  CAS  Google Scholar 

  • Brown PJ, William LR, Cleve F, Stephen X (2008) Efficient mapping of plant height quantitative trait loci in a Sorghum association population with introgressed dwarfing genes. Genetics 180:629–637

    Article  PubMed  Google Scholar 

  • Churchill GA, Doerge RW (1994) Empirical threshold values for quantitative trait mapping. Genetics 138:963–971

    PubMed  CAS  Google Scholar 

  • Cordeiro GM, Casu R, McIntyre CL, Manners JM, Henry RJ (2001) Microsatellite markers from sugarcane (Saccharum spp.) ESTs cross transferable to erianthus and Sorghum. Plant Sci 160:1115–1123

    Article  PubMed  CAS  Google Scholar 

  • Dellaporta SL, Wood J, Hicks JB (1983) A plant DNA minipreparation: Version II. Plant Mol Biol Rep 1:19–21

    Article  CAS  Google Scholar 

  • Feltus FA, Hart GE, Schertz KF (2006) Genetic map alignment and QTL correspondence between inter- and intra-specific Sorghum populations. Theor Appl Genet 112:1295–1305

    Article  PubMed  CAS  Google Scholar 

  • Graham D, Lessman KJ (1966) Effect of height on yield and yield components of two isogenic lines of Sorghum vulgare Pers. Crop Sci 6:372–374

    Article  Google Scholar 

  • Hart GE, Schertz KF, Peng Y, Syed NH (2001) Genetic mapping of Sorghum bicolor (L.) Moench QTLs that control variation in tillering and other morphological characters. Theor Appl Genet 103:1232–1242

    Article  CAS  Google Scholar 

  • Jackson DR, Arthur MF, Davis M, Kresovich S, Lawhon WT, Lipinsky ES, Price M, Rudolph A (1980) Research report on development of sweet Sorghum as an energy crop volume 1: Agricultural task. USDOE, Battele, Columbus. U.S. Department of Commerce

    Google Scholar 

  • Kim JS, Klein PE, Klein RR, Price HJ, Mullet JE, Stelly DM (2005) Chromosome identification and nomenclature of Sorghum bicolor. Genetics 169:1169–1173

    Article  PubMed  CAS  Google Scholar 

  • Klein RR, Rodriguez-Herrera R, Sehlueter JA, Klein PE, Yu ZH, Rooney WL (2001) Identification of genomic regions that affect grain-mould incidence and other traits of agronomic importance in Sorghum. Theor Appl Genet 102:307–319

    Article  CAS  Google Scholar 

  • Kong L, Dong J, Hart GE (2000) Characteristics, linkage Map positions, and allelic differentiation of Sorghum bicolor Moench DNA simple sequence repeats (SSRs). Theor Appl Genet 101:438–448

    Article  CAS  Google Scholar 

  • Lander ES, Green P, Abrahamson J (1987) MAPMAKER: an interactive computer package for constructing primary genetic linkage map of experimental and natural populations. Genomics 1:174–181

    Article  PubMed  CAS  Google Scholar 

  • Li GY, Gu WB and Keith C (2004) Sweet Sorghum, Beijing, China agricultural science and technology press

  • Li ML, Yuyama N, Luo L, Hirata M, Cai HW (2009) In silico mapping of 1758 new SSR markers developed from public genomic sequences for Sorghum. Mol Breeding 24:41–47

    Article  Google Scholar 

  • Lin YR, Schertz KF, Paterson AH (1995) Comparative analysis of QTLs affecting plant height and maturity across the Poaceae, in reference to an interspecific Sorghum population. Genetics 141:391–411

    PubMed  CAS  Google Scholar 

  • Lincoln SE, Daly MJ, Lander ES (1993) Constructing genetic linkage maps with MAPMAKER/EXP version 3.0: A tutorial and reference manual. A Whitehead Institute for biomedical research technical report, 3rd edition. Cambridge, Mass

    Google Scholar 

  • Menz MA, Klein RR, Mullet JE, Obert JA, Unruh NC, Klein PE (2002) A high-density genetic map of Sorghum bicolor (L.) Moench based on 2926 AFLP, RFLP and SSR markers. Plant Mol Biol 48(5–6):483–499

    Article  PubMed  CAS  Google Scholar 

  • Murray SC, Sharma A, Rooney WL, Klein PE, Mullet JE, Mitchell SE, Kresovich S (2008) Genetic improvement of Sorghum as a biofuel feedstock: I. QTL for stem sugar and grain nonstructural carbohydrates. Crop Sci 48:2165–2179

    Article  Google Scholar 

  • Natoli A, Gorni C, Chegdani F, Ajmone Marson P, Colombi C, Lorenzoni C, Marocco A (2002) Identification of QTLs Associated with sweet Sorghum quality. Maydica 47:311–322

    Google Scholar 

  • Parida SK, Raj Kumar KA, K.Singh VDN, Mohapatra T (2006) Unigene derived microsatellite markers for the cereal genomes. Theor Appl Genet 112:808–817

    Article  PubMed  CAS  Google Scholar 

  • Pereira MG, Lee M (1995) Identification of genomic regions affecting plant height in Sorghum and maize. Theor Appl Genet 90:380–388

    Article  CAS  Google Scholar 

  • Quinby JR, Karper RE (1954) Inheritance of height in Sorghum. Agron J 46:211–216

    Article  Google Scholar 

  • Rami JF, Dufour P, Trouche G, Fliedel G, Mestres C, Davrieux F, Blanchard P, Hamon P (1998) Quantitative trait loci for grain quality, productivity, morphological and agronomical traits in Sorghum (Sorghum bicolor L. Moench). Theor Appl Genet 97:605–616

    Article  CAS  Google Scholar 

  • Ritter KB, Jordan DR, Chapman SC, Godwin ID, Mace ES, McIntyre CL (2008) Identification of QTL for sugar-related traits in a sweet×grain Sorghum (Sorghum bicolor L. Moench) recombinant inbred population. Mol Breeding 22:367–384

    Article  Google Scholar 

  • Rooney WL, Blumenthal J, Bean B, Mullet JE (2007) Designing Sorghum as a dedicated bioenergy feedstock. Biofuels Bioprod Bioref 1:147–157

    Article  CAS  Google Scholar 

  • Schloss SJ, Mitchell SE, White GM (2002) Characterization of RFLP probe sequences for gene discovery and SSR development in Sorghum bicolor (L.) Moench. Theor Appl Genet 105(6–7):912–920

    PubMed  CAS  Google Scholar 

  • Shiringani AL, Frisch M, Friedt W (2010) Genetic mapping of QTLs for sugar-related traits in a RIL population of Sorghum bicolor L. Moench. Theor Appl Genet 121:323–336

    Article  PubMed  CAS  Google Scholar 

  • Taramino G, Tarchini R, Ferrario S, Lee M, Pe ME (1997) Characterization and mapping of simple sequence repeats (SSRs)in Sorghum bicolor. Theor Appl Genet 95:66–72

    Article  CAS  Google Scholar 

  • Voorrips R (2002) MapChart: software for the graphical presentation of linkage maps and QTLs. J Hered 93:77–78

    Article  PubMed  CAS  Google Scholar 

  • Wang SC, Basten J, Zeng ZB (2010) Windows QTL Cartographer 2.5. Department of Statistics. North Carolina State University, Raleigh, NC. http://statgen.ncsu.edu/qtlcart/WQTL.Cart.htm

  • Wu YQ, Huang YH (2006) An SSR genetic map of Sorghum bicolor (L.) Moench and its comparison to a published genetic map. Genome 50:84–89

    Google Scholar 

Download references

Acknowledgments

This study was supported by Special fund for agro-scientific research in public interest from Ministry of Agriculture of China, a project of National Science and Technology Pillar Program (2007BADA7B01) from Ministry of Science and Technology of China, and the Earmarked Fund for China Agriculture Research System (CARS-06). The authors are grateful to the critical reading of reviewers, and to Professor Du Rui-hong, Millet Research Institute, Hebei Academy of Agro-forestry Sciences, for his kindly providing the Sorghum parents, L-Tian and Shihong137.

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Authors and Affiliations

  1. College of Agronomy, Shandong Agricultural University, Taian, 271018, China

    Yan-an Guan & Hong-gang Wang

  2. Crop Research Institute, Shandong Academy of Agricultural Sciences, Jinan, 250100, China

    Yan-an Guan, Hai-lian Wang, Ling Qin, Hua-wen Zhang, Yan-bing Yang & Ru-yu Li

  3. Dezhou Academy of Agricultural Sciences, Dezhou, 253000, China

    Feng-ju Gao

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  1. Yan-an Guan
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  2. Hai-lian Wang
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  3. Ling Qin
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  4. Hua-wen Zhang
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  5. Yan-bing Yang
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  7. Ru-yu Li
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  8. Hong-gang Wang
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Correspondence to Hong-gang Wang.

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Guan, Ya., Wang, Hl., Qin, L. et al. QTL mapping of bio-energy related traits in Sorghum . Euphytica 182, 431–440 (2011). https://doi.org/10.1007/s10681-011-0528-5

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