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Molecular cytogenetic analysis of radiation-induced wheat-rye terminal and intercalary chromosomal translocations and the detection of rye chromatin specifying resistance to Hessian fly

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Abstract

Radiation-induced wheat-rye chromosome translocation lines resistant to Hessian fly, Mayetiola destructor (say), were analyzed by in situ hybridization using total genomic and highly repetitive rye DNA probes pSc119 and pSc74. In situ hybridization analysis revealed the exact locations of the translocation breakpoints and allowed the estimation of the sizes of the transferred rye segments. T6BS·6BL-6RL and T4BS· 4BL-6RL are terminal translocations with either most of the complete long arm of rye chromosome 6R or only the distal 57% of the 6RL arm attached to the long arms of wheat chromosomes 6B and 4B, respectively. The breakpoint in T6BS·6BL-6RL is located at a fraction length (FL) of 0.11 in the long arm of T6BS 6BL-6RL and at FL 0.46 in the long arm of T4BS·4BL-6RL. Ti4AS·4AL-6RL-4AL is an intercalary translocation with the breakpoint located at FL 0.06 in the long arm of wheat chromosome 4A. The inserted 6RL segment, with the Hessian fly resistance gene, has a size of 0.7 μm, and is the smallest and, so far, the first radiation-induced intercalary translocation identified in wheat.

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References

  • Amri A, Cox TS, Gill BS, Hatchett JH (1990) Chromosomal location of the Hessian fly resistance gene H20 in ‘Jori’ durum wheat. J Hered 81:72–72

    Google Scholar 

  • Anamthawat-Jónsson K, Schwarzacher T, Leitch AR, Bennett MD, Heslop-Harrison JS (1990) Discrimination between closely related Triticeae species using genomic DNA as a probe. Theor Appl Genet 79:721–728

    Google Scholar 

  • Bedbrook JR, Jones J, O'Dell M, Thompson RD, Flavell RB (1980) A molecular description of telomeric heterochromatin in Secale species. Cell 19:545–560

    Google Scholar 

  • Clark M, Karp A, Archer S (1989) Physical mapping of the β-hordein loci on barley chromosome 5 by in situ hybrididzation. Genome 32:925–929

    Google Scholar 

  • Driscoll CJ (1968) Alien transfer by irradiation and meiotic control. In: Proc 3rd Int Wheat Genet Symp, Canberra, Australia, pp 196–203

  • Driscoll CJ, Jensen MF (1964) Characteristics of leaf rust resistance transferred from rye to wheat. Crop Sci 4:372–374

    Google Scholar 

  • Friebe B, Hatchett JH, Sears RG, Gill BS (1990) Transfer of Hessian fly resistance from ‘Champon’ rye to hexaploid wheat via a 2BS·2RL wheat-rye chromosome translocation. Theor Appl Genet 79:385–389

    Google Scholar 

  • Friebe B, Hatchett JH, Mukai Y, Gill BS, Sebesta EE (1991a) X-ray induced transfer of Hessian fly resistance from ‘Balbo’ rye to hexaploid wheat analyzed by the C-banding techniques. In: Kimber G (ed) Proc 2nd Int Symp Chromosome Eng in Plants, Columbia, Missouri, August 13–15, 1990, pp 189–194

  • Friebe B, Mukai Y, Dhaliwal HS, Martin TJ, Gill BS (1991b) Identification of alien chromatin specifying resistant to wheat streak mosaic and greenbug in wheat germplasm by C-banding and in situ hybridization. Theor Appl Genet 81:381–389

    Google Scholar 

  • Friebe B, Hatchett JH, Gill BS, Mukai Y, Sebesta EE (1991c) Transfer of Hessian fly resistance from rye to wheat via radiation-induced terminal and intercalary chromosomal translocations. Theor Appl Genet 83:33–40

    Google Scholar 

  • Friebe B, Zeller FJ, Mukai Y, Forster BP, Bartos P, McIntosh RA (1992) Characterization of rust-resistant wheat-Agropyron intermedium derivatives by C-banding, in situ hybridization and isozyme analysis. Theor Appl Genet 83:775–782

    Google Scholar 

  • Friebe B, Jiang J, Gill BS, Dyck PL (1993) Radiation-induced nonhomoeologous wheat-Agropyron intermedium chromosomal translocations conferring resistance to leaf rust. Theor Appl Genet (in press)

  • Gallun RL (1977) Genetic basis of Hessian fly epidemics. Ann NY Acad Sci 287:223–229

    Google Scholar 

  • Gill BS, Friebe B, Endo TR (1991) Standard karyotype and nomenclature system for description of chromosome bands and structural aberrations in wheat (Triticum aestivum L.). Genome 34:830–839

    Google Scholar 

  • Hatchett JH, Gallun RL (1968) Frequency of Hessian fly, Mayetiola destructor, races in field populations. J Econ Entomol 61:1446–1449

    Google Scholar 

  • Heslop-Harrison JS, Leitch AR, Schwarzacher T, Anamthawat-Jónsson K (1990) Detection and characterization of 1B/1R translocations in hexaploid wheat. Heredity 65:385–392

    Google Scholar 

  • Jones JD, Flavell RB (1982) The mapping of highly repeated DNA families and their relationship to C-bands in chromosomes of Secale cereale. Chromosoma 86:595–612

    Google Scholar 

  • Knott DR (1961) The transfer of genes for disease resistance from alien species to wheat by induced translocations. In: Mutation breeding for disease resistance. IAEA, Vienna, pp 67–77

    Google Scholar 

  • Knott DR (1964) The effect on wheat of an Agropyron chromosome carrying rust resistance. Can J Genet Cytol 6:500–507

    Google Scholar 

  • Lapitan NLV, Sears RG, Rayburn AL, Gill BS (1986) Wheat-rye translocations. Detection of chromosome breakpoints by in situ hybridization with a biotin-labeled DNA probe. J Hered 77:415–419

    Google Scholar 

  • Lapitan NLV, Gill BS, Sears RG (1987) Genomic and phylogenic relationships among rye and perennial species in the Triticeae. Crop Sci 27:682–687

    Google Scholar 

  • Le HT, Armstrong KC (1991) In situ hybridization as a rapid means to assess meiotic pairing and detection of alien DNA transfers in interphase cells of wide crosses involving wheat and rye. Mol Gen Genet 225:33–37

    Google Scholar 

  • Le HT, Armstrong KC, Miki B (1989) Detection of rye DNA in wheat-rye hybrids and wheat translocation stocks using total genomic DNA as a probe. Plant Mol Biol Rep 7:150–158

    Google Scholar 

  • McIntyre CL, Pereira S, Moran LB, Appels R (1990) New Secale cereale (rye) DNA derivatives for the detection of rye chromosome segments in wheat. Genome 33:635–640

    Google Scholar 

  • Mukai Y, Gill BS (1991) Detection of barley chromatin added to wheat by genomic in situ hybridization. Genome 34:448–452

    Google Scholar 

  • Mukai Y, Endo TR, Gill BS (1990) Physical mapping of the 5S rRNA multigene family in common wheat. J Hered 81:290–295

    Google Scholar 

  • Mukai Y, Friebe B, Hatchett JH, Gill BS (1991a) Detection of rye chromatin in wheat specifying resistance to Hessian fly by in situ hybridization using total rye genomes DNA probes. In: Kimber G (ed) Proc 2nd Int Symp Chromosome Eng in Plants, Columbia, MO, August 13–15, 1990, pp 184–188

  • Mukai Y, Endo TR, Gill BS (1991b) Physical mapping of the 18S· 26S rRNA multigene family in common wheat: Identification of a new locus. Chromosoma 100:71–78

    Google Scholar 

  • Mukai Y, Friebe B, Gill BS (1992) Comparison of C-banding patterns and in situ hybridization sites using highly repetitive and total genomic rye DNA probes of ‘Imperial’ rye chromosomes added to ‘Chinese Spring’ wheat. Jpn J Genet 67:71–83

    Google Scholar 

  • Painter RH (1951) Insect resistance in crop plants. McMillian, New York

    Google Scholar 

  • Rayburn AL, Gill BS (1985a) Use of biotin-labeled probes to map specific DNA sequences on wheat chromosomes. J Hered 76:78–81

    Google Scholar 

  • Rayburn AL, Gill BS (1985b) Molecular evidence for the origin and evolution of chromosome 4A in polyploid wheats. Can J Genet Cytol 27:246–250

    Google Scholar 

  • Rayburn AL, Gill BS (1987) Molecular analysis of the D-genome of the Triticeae. Theor Appl Genet 73:385–388

    Google Scholar 

  • Rogers SO, Bendich AJ (1988) Extraction of DNA from plant tissues. In: Gelvin SB, Schilperoot RA (eds) Plant molecular biology manual A6. Kluwer Academic Publishers, Dordrecht, pp 1–10

    Google Scholar 

  • Rogowsky PM, Manning S, Liu J-Y, Langridge P (1991) The R173 family of rye-specific repetitive DNA sequences: a structural analysis. Genome 34:88–95

    Google Scholar 

  • Sears ER (1956) The transfer of leaf rust resistance from Aegilops umbellulata to wheat. Brookhaven Symp Biol 9:1–21

    Google Scholar 

  • Sears ER (1972) Chromosome engineering in wheat. Stadler Symposia, Vol 4, University of Columbia, Missouri, pp 23–38

    Google Scholar 

  • Sharma D, Knott DR (1966) The transfer of leaf-rust resistance from Agropyron to Triticum by irradiation. Can J Genet Cytol 8:137–143

    Google Scholar 

  • Sosa O-Jr (1981) Biotypes J and L of the Hessian fly discovered in an Indiana wheat field. J Econ Entomol 74:180–182

    Google Scholar 

  • Wienhues A (1973) Translocation between wheat chromosomes and an Agropyron chromosome conditioning rust resistance. In: Sears ER, Sears LMS (eds) Proc 4th Int Wheat Genet Symp, University of Missouri, Columbia, Missouri, pp 201–207

  • Xin ZY, Brettell RIS, Cheng ZM, Waterhouse PM, Appels R, Banks PM, Zhou GH, Chen X, Larkin PJ (1988) Characterization of a potential source of barley yellow dwarf virus resistance for wheat. Genome 30:250–257

    Google Scholar 

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Author notes

  1. Y. Mukai

    Present address: Osaka Kyoiku University, 4-698-1, Asahigaoka, Kashiwara, 582, Osaka, Japan

  2. M. Yamamoto

    Present address: Kansai Women's Junior College, 3-11-1, Asahigaoka, 582, Kashiwara, Osaka, Japan

Authors and Affiliations

  1. Department of Plant Pathology, Kansas State University, Throckmorton Hall, 66505-5502, Manhatten, KS, USA

    B. Friebe & B. S. Gill

  2. USDA-ARS and Departments of Entomology, Waters Hall, Kansas State University, 66505-5502, Manhatten, KS, USA

    J. H. Hatchett

Authors
  1. Y. Mukai
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  2. B. Friebe
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  3. J. H. Hatchett
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  4. M. Yamamoto
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  5. B. S. Gill
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Mukai, Y., Friebe, B., Hatchett, J.H. et al. Molecular cytogenetic analysis of radiation-induced wheat-rye terminal and intercalary chromosomal translocations and the detection of rye chromatin specifying resistance to Hessian fly. Chromosoma 102, 88–95 (1993). https://doi.org/10.1007/BF00356025

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  • DOI: https://doi.org/10.1007/BF00356025

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