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Using three selected overlapping RILs to fine-map the yield component QTL on Chro.D8 in Upland cotton

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Abstract

Cotton yield improvement is vital to fulfill rising global demands. The identification of major quantitative trait loci (QTL) for yield components was helpful in molecular marker-assisted selection (MAS) to improve cotton yield. We previously identified a densely populated QTL region for fiber qualities and yield components on chromosome D8 (Chro.D8) of Upland cotton from a (7235 × TM-1)RIL. In the present study, to fine-map yield component QTLs, we chose three overlapped recombinant inbred lines (RILs) with different intervals included the yield component QTLs, and backcrossed each line with TM-1 to develop three large sized mapping populations. Phenotypic data for yield components were collected in Nanjing (JES/NAU) and Xinjiang (BES/XJ) in 2006 and 2007. Three simple sequence repeat (SSR) genetic linkage maps on chro.D8 were constructed using 907 individuals in (7TR-133 × TM-1)F2 (Pop A), 670 in (7TR-132 × TM-1)F2 (Pop B), and 940 in (7TR-214 × TM-1)F2 (Pop C). Three stable QTLs for boll size, two for lint percentage and one for boll number per plant,were detected on chro.D8 following analysis of three RIL backcrossed F2/F2:3 progeny at JES/NAU and BES/XJ although their cultivation practices differ greatly between these two cotton-growing regions. One QTL for boll number per plant exhibited a phenotypic variance (PV) of 5.6–10.1%, three QTLs for boll size exhibited 15.0–35.5% PV and two lint percentage QTLs exhibited 10.9–19.3% PV. Negative correlation between lint yield and fiber strength was confirmed.

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References

  • Chen H, Qian N, Guo WZ, Song QP, Li BC, Deng FJ, Dong CG, Zhang TZ (2009) Using three overlapped RILs to dissect genetically clustered QTL for fiber strength on chro.24 in Upland cotton. Theor Appl Genet 119:605–612

    Article  PubMed  Google Scholar 

  • Guo WZ, Cai CP, Wang CP, Han ZG, Song XL, Kai Wang, Niu XW, Wang C, Lu KY, Ben S, Zhang TZ (2007) A microsatellite-based, gene-rich linkage map reveals genome structure, function and evolution in Gossypium. Genetics 176:527–541

    Article  CAS  PubMed  Google Scholar 

  • Jiang CX, Wright RJ, El-Zik KM, Paterson AH (1998) Polyploid formation created unique convenues for response to selection in Gossypium (cotton). Proc Acad Natl Sci USA 95:4419–4424

    Article  CAS  Google Scholar 

  • Kohel RJ, Richmond TR, Lewis CF (1970) Texas marker-1. Description of a genetic standard for Gossypium hirsutum L. Crop Sci 10:670–671

    Article  Google Scholar 

  • Lacape JM, Nguyen TB, Thibivilliers S, Bojinnov TB, Courtois B, Cantrell RG, Burr B, Hau B (2003) A combined RFLP-SSR-AFLP map of tetraploid cotton based on a Gossypium hirsutum × Gossypium barbadense backcross population. Genome 46:612–626

    Article  CAS  PubMed  Google Scholar 

  • Lander ES, Botstein D (1986) Strategies for studying heterogeneous genetic traits in humans by using a linkage map of restriction fragment length polymorphisms. Proc Natl Acad Sci USA 83:7353–7357

    Article  CAS  PubMed  Google Scholar 

  • McCouch SR, Cho YG, Yano PE, Blinstrub M, Morishima H, Kinoshita T (1997) Report on QTL nomenclature. Rice Genet Newslett 14:11–13

    Google Scholar 

  • Mei M, Syed NH, Gao W, Thaxton PM, Smith CW, Stelly DM, Chen ZJ (2004) Genetic mapping and QTL analysis of fiber-related traits in cotton (Gossypium). Theor Appl Genet 108:280–291

    Article  CAS  PubMed  Google Scholar 

  • Meredith WR Jr (1984) Quantitative genetics. In: Kohel RJ, Lewis CF (eds) Cotton. Am Soc Agro, Inc., Crop Sci Soc AM, Inc., and Soil Sci Soc AM, Inc., Madison, WI, pp 132–150

    Google Scholar 

  • Paterson AH, Lander SE, Hewitt JD, Peterson S, Lincoln SE, Tanksely SD (1988) Resolution of quantitative traits into Mendelian factors using a complete linkage map of restriction fragement length polymorphisms. Nature 355:721–726

    Article  Google Scholar 

  • SAS institute (1989) SAS/STAT user’s guide version 6, 4th edn. SAS Institute, Cary, NC

    Google Scholar 

  • Shappley ZW, Jenkins JN, Zhu J, McCarty JC (1998) Quantitative trait loci associated with agronomic and fiber traits of upland cotton. J Cotton Sci 2:153–163

    CAS  Google Scholar 

  • Shen XL, Guo WZ, Zhu XF, Yuan YL, Yu JZ, Kohel RJ, Zhang TZ (2005) Molecular mapping of QTL for fiber qualities in three diverse lines in Upland cotton using SSR markers. Mol Breed 15:169–181

    Article  CAS  Google Scholar 

  • Shen XL, Guo WZ, Lu QX, Zhu XF, Yuan YL, Zhang TZ (2007) Genetic mapping of quantitative trait loci for fiber quality and yield trait by RIL approach in Upland cotton. Euphytica 155:371–380

    Article  CAS  Google Scholar 

  • Ulloa M, Meredith WR (2000) Genetic linkage map and QTL analysis of agronomic and fiber quality traits in an intraspecific population. J Cotton Sci 4:161–170

    CAS  Google Scholar 

  • Ulloa M, Cantrell RG, Pency RG (2000) QTL analysis of stomatal conductance and relationship to lint yield in an interspecific cotton. J Cotton Sci 4:10–18

    CAS  Google Scholar 

  • Ulloa M, Meredith WR, Shapplet ZW, Kahler AL (2002) RPLF genetic linkage maps from four F2:3 population and a joinmap of Gossypium hirsutum L. Thero Appl Genet 104:200–208

    Article  CAS  Google Scholar 

  • Ulloa M, Saha S, Jenkin N, Meredith WR, McCarty JC, Stelly DM (2005) Chromosomal assignment of RFLP linkage groups harboring important QTL on an intraspecific cotton (Gossypium hirsutum L.) joinmap. J Hered 96:132–144

    Article  CAS  PubMed  Google Scholar 

  • Van Ooijen JW (2004) MapQTL version 5.0: software for the mapping of quantitative trait loci in experiment population. Kyazma BV, Wageningen, the Netherlands

  • Van Ooijen JW, Voorrips RE (2001) JoinMap version 3.0: software for the calculation of genetic linkage maps. Plant Research International, Wageningen, the Netherlands

  • Varshney RK, Tuberosa R (2007) Genomics-assisted crop improvement, vol. 1: Genomics approaches and platforms. Springer, Netherlands, pp 1–12

  • Wang K, Song XL, Han ZG, Guo WZ, Yu JZ, Sun J, Pan JJ, Kohel RJ, Zhang TZ (2006) Complete assignment of the chromosomes of G. hirsutum L. by BAC-FISH and Translocations. Theor Appl Genet 113:73–80

    Article  CAS  PubMed  Google Scholar 

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Acknowledgements

This work was financially supported in part by grants from the High-tech program 863 (2006AA100105), Jiangsu province key project (BE2008310, BG2006307), and the 111 Project (B08025).

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Authors and Affiliations

  1. National Key Laboratory of Crop Genetics and Germplasm Enhancement, Cotton Research Institute, Nanjing Agricultural University, Nanjing, 210095, Jiangsu Province, China

    Hong Chen, Neng Qian, Wangzhen Guo & Tianzhen Zhang

  2. Cotton Research Institute, Xinjiang Academy of Agriculture and Reclamation Sciences, Xinjiang, 832000, China

    Qingping Song, Baocheng Li, Fujun Deng & Cunguang Dong

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  1. Hong Chen
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  2. Neng Qian
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  3. Wangzhen Guo
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  4. Qingping Song
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  5. Baocheng Li
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  6. Fujun Deng
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  7. Cunguang Dong
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  8. Tianzhen Zhang
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Correspondence to Tianzhen Zhang.

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Chen, H., Qian, N., Guo, W. et al. Using three selected overlapping RILs to fine-map the yield component QTL on Chro.D8 in Upland cotton. Euphytica 176, 321–329 (2010). https://doi.org/10.1007/s10681-010-0204-1

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