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Molecular mapping of Pc68, a crown rust resistance gene in Avena sativa

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Abstract

Crown rust, which is caused by Puccinia coronata f. sp. avenae, P. Syd. & Syd., is the most destructive disease of cultivated oats (Avena sativa L.) throughout the world. Resistance to the disease that is based on a single gene is often short-lived because of the extremely great genetic diversity of P. coronata, which suggests that there is a need to develop oat cultivars with several resistance genes. This study aimed to identify amplified fragment length polymorphism AFLP markers that are linked to the major resistance gene, Pc68, and to amplify the F6 genetic map from Pc68/5*Starter × UFRGS8. Seventy-eight markers with normal segregation were discovered and distributed in 12 linkage groups. The map covered 409.4 cM of the Avena sativa genome. Two AFLP markers were linked in repulsion to Pc68: U8PM22 and U8PM25, which flank the gene at 18.60 and 18.83 centiMorgans (cM), respectively. The marker U8PM25 is located in the linkage group 4_12 in the Kanota × Ogle reference oat population. These markers should be useful for transferring Pc68 to genotypes with good agronomic characteristics and for pyramiding crown rust resistance genes.

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Acknowledgment

The authors thank to the Brazilian Council for Scientific and Technological Development (CNPq) for the scholarship provided to the first author.

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Author notes

  1. Ana B. Locatelli

    Present address: DuPont do Brasil S/A, Pioneer Seeds, Passo Fundo, RS, Brazil

Authors and Affiliations

  1. Departamento de Plantas de Lavoura, Federal University of Rio Grande do Sul, Programa de Pós-graduação em Fitotecnia, P.O. Box 15 100, Porto Alegre, RS, CEP 91501-970, Brazil

    Franceli R. Kulcheski, Ana B. Locatelli, Luiz C. Federizzi & Carla A. Delatorre

  2. Departamento de Fitossanidade, Federal University of Rio Grande do Sul, P.O. Box 15 100, Porto Alegre, RS, CEP 91501-970, Brazil

    Felipe A. S. Graichen & José A. Martinelli

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  1. Franceli R. Kulcheski
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  2. Felipe A. S. Graichen
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  3. José A. Martinelli
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  4. Ana B. Locatelli
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  6. Carla A. Delatorre
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Correspondence to Carla A. Delatorre.

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Part of the Master of Science dissertation of Franceli R. Kulcheski.

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Kulcheski, F.R., Graichen, F.A.S., Martinelli, J.A. et al. Molecular mapping of Pc68, a crown rust resistance gene in Avena sativa . Euphytica 175, 423–432 (2010). https://doi.org/10.1007/s10681-010-0198-8

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