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Inheritance and mapping of a powdery mildew resistance gene introgressed from Avena macrostachya in cultivated oat

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Abstract

The powdery mildew resistance from Avena macrostachya was successfully introgressed into hexaploid oat (A. sativa). Genetic analysis of F1, F2, F3 and BC1 populations from two powdery-mildew resistant introgression lines revealed that the resistance is controlled by a dominant gene, tentatively designated Eg-5. Molecular marker analysis was conducted using bulked-segregant analysis in two segregating F3 populations. One codominant simple sequence repeats (SSR) marker AM102 and four AFLP-derived PCR-based markers were successfully developed. The SSR marker AM102 and the STS marker ASE41M56 were linked to the gene Eg-5, with genetic distances of 2 and 0.4 cM, respectively, in both mapping populations. Three STS markers (ASE45M56, ASE41M61, ASE36M55) co-segregated with Eg-5 in one population while two (ASE45M56, ASE36M55) of them linked to Eg-5 with a genetic distance of 1 cM in another population. The gene was further mapped to be in a region corresponding to linkage group 22_44+18 in the Kanota × Ogle (KO) hexaploid oat map by comparative mapping. To our knowledge, this is the first report of mapping powdery-mildew resistance in hexaploid oat. The new resistance source of A. macrostachya, together with the tightly linked markers identified here, could be beneficial in oat breeding programmes.

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Acknowledgements

We thank Dr. P. Wehling, Dr. B. Hackauf and Dr. B. Ruge for their valuable suggestions and helpful advice.

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Authors and Affiliations

  1. Institute of Agricultural Crops, Federal Centre for Breeding Research on Cultivated Plants (BAZ), Rudolf-Schick-Platz 3a, 18190, Gross Lüsewitz, Germany

    J. Yu & M. Herrmann

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  1. J. Yu
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  2. M. Herrmann
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Correspondence to M. Herrmann.

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Communicated by B. Friebe

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Yu, J., Herrmann, M. Inheritance and mapping of a powdery mildew resistance gene introgressed from Avena macrostachya in cultivated oat. Theor Appl Genet 113, 429–437 (2006). https://doi.org/10.1007/s00122-006-0308-0

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  • DOI: https://doi.org/10.1007/s00122-006-0308-0

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