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Association mapping and marker development of the candidate genes (1 → 3),(1 → 4)-β-d-Glucan-4-glucanohydrolase and (1 → 4)-β-Xylan-endohydrolase 1 for malting quality in barley

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Abstract

Cell wall degradation is a crucial process within the malting process of barley. Therefore, the haplotype diversity of genes for two cell wall degrading enzymes, (1 → 3),(1 → 4)-β-d-Glucan-4-glucanohydrolase and (1 → 4)-β-Xylan-endohydrolase 1, was investigated and associations to malting quality parameters were performed. The (1 → 3),(1 → 4)-β-d-Glucan-4-glucanohydrolase gene glb2 had two major haplotypes defined by three SNPs and one INDEL, which explained 8.9 and 9.5% of the total variation of malt extract content and viscosity in the spring barley gene pool, respectively. The most significant associations of (1 → 4)-β-Xylan-endohydrolase 1 gene X-1 were found for diastatic power, saccharification VZ45 and soluble nitrogen with 18, 12 and 8% of the total variation explained by SNP3 in the spring barleys. High-throughput markers were developed for both genes which can be used for marker assisted selection.

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Acknowledgments

We thank A. Flieger, E. Weiß and S. Kirsten for excellent technical assistance. The project was funded by a grant (project no. 0313125A) in the GABI program of the BMBF.

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Authors and Affiliations

  1. Leibniz Institute for Plant Genetics and Crop Plant Research (IPK), Corrensstr. 3, 06466, Gatersleben, Germany

    Inge E. Matthies, Stephan Weise & Marion S. Röder

  2. SAATEN-UNION Resistenzlabor GmbH (SURL), Hovedisser Straße 92, 33818, Leopoldshohe, Germany

    Jutta Förster

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  1. Inge E. Matthies
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  2. Stephan Weise
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Correspondence to Inge E. Matthies.

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The three Supplemental Tables can also be viewed in the following links.

http://pgrc.ipk-gatersleben.de/glb.

http://pgrc.ipk-gatersleben.de/X-1.

http://pgrc.ipk-gatersleben.de/Phendat.

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Matthies, I.E., Weise, S., Förster, J. et al. Association mapping and marker development of the candidate genes (1 → 3),(1 → 4)-β-d-Glucan-4-glucanohydrolase and (1 → 4)-β-Xylan-endohydrolase 1 for malting quality in barley. Euphytica 170, 109–122 (2009). https://doi.org/10.1007/s10681-009-9915-6

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