Skip to main content
SpringerLink
Log in

Inheritance mode of male sterility in bunching onion (Allium fistulosum L.) accessions

  • Published:
Euphytica Aims and scope Submit manuscript

Abstract

Cytoplasmic male sterility (CMS) is an indispensable trait for F1 hybrid seed production in bunching onion (Allium fistulosum L.). Expansion of the cytoplasmic diversity of F1 hybrid cultivars by introduction of various CMS resources has great potential to eliminate vulnerability to cytoplasm type-specific diseases. This study aimed to evaluate appearance frequency of male sterile plants in several bunching onion accessions and to identify CMS resources. In eight (‘Nogiwa Aigara’, ‘Bansei Hanegi’, ‘Amarume’, ‘Kimnung’, ‘Zhangqiu’, ‘INT/CHN/1990/GOTOU’, ‘Natsunegi’ and ‘Guangzhou’) of 135 accessions collected from Japan, China, Mongolia, Korea and Taiwan, male sterile plants appeared with varied frequencies from 1.7% (‘Nogiwa Aigara’ and ‘Bansei Hanegi’) to 24.5% (‘Zhangqiu’). The inheritance mode of Zhangqiu- and Guangzhou-derived male sterility was confirmed to be CMS by sib-crossings and interbreed crossings. Microscopic examination of microsporogenesis in the CMS plants revealed that microspore protoplasm rapidly degenerated without mitotic division after the release of microspores from tetrads. The CMS germplasm described here would be useful for the development of “A” lines to be used in F1 hybrid seed production of bunching onion. Male fertility in ‘Nogiwa Aigara’, ‘Bansei Hanegi’, ‘Kimnung’, ‘INT/CHN/1990/GOTOU’ and ‘Natsunegi’ was verified to be controlled by a single fertility restoration locus.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1

Similar content being viewed by others

References

  • Berninger E (1965) Contribution to the study of male sterility in the onion (Allium cepa L.). Ann Amelior Plant 15:183–199

    Google Scholar 

  • Buiteveld J, Suo Y, van Lookeren Campagne MM, Creemers-Molenaar J (1998) Production and characterization of somatic hybrid plants between leek (Allium ampeloprasum L.) and onion (Allium cepa L.). Theor Appl Genet 96:765–775

    Article  CAS  Google Scholar 

  • Desloire S, Gherbi H, Laloui W, Mardour S, Clouet V, Cattolico L, Falentin C, Giancola S, Renard M, Budar F, Small I, Caboche M, Delourme R, Bendshmane A (2003) Identification of the fertility restoration locus, Rfo, in radish, as a member of the pentatricopeptide-repeat protein family. Embo Rep 4:588–594

    Article  CAS  PubMed  Google Scholar 

  • Edwardson JR (1970) Cytoplasmic male sterility. Bot Rev 22:296–738

    Google Scholar 

  • Engelke T, Terefe D, Tatlioglu T (2003) A PCR-based marker system monitoring CMS-(S), CMS-(T) and (N)-cytoplasm in the onion (Allium cepa L.). Theor Appl Genet 107:162–167

    CAS  PubMed  Google Scholar 

  • Havey MJ (1995) Identification of cytoplasms using the polymerase chain reaction to aid in the extraction of maintainer lines from open-pollinated populations of onion. Theor Appl Genet 90:263–268

    Article  CAS  Google Scholar 

  • Havey MJ (1999) Seed yield, floral morphology, and lack of male-fertile restoration of male sterile onion (Allium cepa) population possessing the cytoplasm of Allium galanthum. J Am Soc Hortic Sci 124:626–629

    Google Scholar 

  • Havey MJ (2000) Diversity among male-sterility-inducing and male-fertile cytoplasms of onion. Theor Appl Genet 101:778–782

    Article  CAS  Google Scholar 

  • Holford P, Craft JH, Newbury HJ (1991) Differences between, and possible origins of, the cytoplasms found in fertile and male-sterile onions (Allium cepa L.). Theor Appl Genet 82:737–744

    Google Scholar 

  • Inden H, Asahira T (1990) Japanese bunching onion (Allium fistulosum L.). In: Rabinowitch HD, Brewster JL (eds) Onions and allied crops, vol 2. CRC Press, Boca Raton, Florida, pp 159–178

    Google Scholar 

  • Isshiki S, Kawajiri N (2002) Effect of cytoplasm of Solanum violaceum Ort. on fertility of eggplant (S. melongena L.). Sci Hortic 93:9–18

    Article  Google Scholar 

  • Japan Horticultural Production and Research Institute (2006) In: Itou T (ed) Sosai no Shinhinshu, vol 16. Seibundoushinkousha Publ., Tokyo, Japan, pp 157–164 (in Japanese)

  • Japan Seed Trade Association (2009) In: Matsunaga K (ed) Yasai Hinshu Meikan. Japan Seed Trade Association, Tokyo (in Japanese)

  • Jones HA, Clarke AE (1943) Inheritance of male-sterility in the onion and the production of hybrid seed. Proc Am Soc Hortic Sci 43:189–194

    Google Scholar 

  • Jones HA, Emsweller SL (1937) A male sterile onion. Proc Am Soc Hortic Sci 34:582–585

    Google Scholar 

  • Katsumata H (1985) Cytoplasmic male sterility and F1 hybrid breeding. In: Yamaguchi H (ed) Cytoplasmic male sterility. CMC press, Tokyo, Japan, pp 183–194

    Google Scholar 

  • Kik C, Wietsma WA, Verbeek WHJ (1998) Onion. In: Banga SS, Banga SK (eds) Hybrid cultivar development: concepts and methodologies. Narosa Publ. House, New Delhi, India, pp 476–485

    Google Scholar 

  • Kim S, Lee EL, Cho DY, Han T, Bang H, Patil BS, Ahn YK, Yoon MK (2009) Identification of a novel chimeric gene, orf 725, and its use in development of a molecular marker for distinguishing among three cytoplasm types in onion (Allium cepa L.). Theor Appl Genet 118:433–441

    Article  CAS  PubMed  Google Scholar 

  • Kumazawa S, Katsumata H (1965) Negi (Japanese bunching onion). In: Kumazawa S (ed) Sosai-engei kakuron (Vegetable crops). Yokendo Publ., Tokyo, pp 280–289 (in Japanese)

  • Levingth CS III (1991) The Texas cytoplasm of maize: cytoplasmic male sterility and disease susceptibility. Science 250:942–947

    Article  Google Scholar 

  • Martin WJ, McCallum J, Shigyo M, Jakne J, Kuhl JC, Yamane N, Pither-Joyce M, Gökçe AF, Sink KC, Town CD, Havey MJ (2005) Genetic mapping of expressed sequences in onion and in silico comparisons with rice show scant colinearity. Mol Gen Genom 274:197–204

    Article  CAS  Google Scholar 

  • McCallum J, Pither-Joyce M, Clarke A, Kik C, Van Heusden S, Jakse J, Havey MJ (2005) Towards a framework PCR-based map of onion (Allium cepa L.). Acta Hortic IV IS on Edible Alliaceae 688:173–178

    CAS  Google Scholar 

  • McVetty PBE (1997) Cytoplasmic male sterility. In: Shivanna KR, Sawhney VK (eds) Pollen biotechnology for crop production and improvement. Cambridge University Press, Winnipeg, Manitoba, pp 155–182

    Chapter  Google Scholar 

  • Ministry of Agriculture, Forestry and Fisheries (2008) Shuyou-nousanbutsu no Sanshutsugaku (2006) (in Japanese). http://www.e-stat.go.jp/SG1/estat/List.do?lid=000001031687

  • Moue T, Uehara T (1985) Inheritance of cytoplasmic male sterility in Allium fistulosum L. (welsh onion). J Jpn Soc Hortic Sci 53:432–437 (in Japanese)

    Google Scholar 

  • Nishimura Y, Shibano M (1972) Male sterility in A. fistulosum L. Cytological and anatomical studies. J Jpn Soc Hortic Sci (Suppl 1):180–181 (in Japanese)

  • Sato Y (1998) PCR amplification of CMS-specific mitochondrial nucleotide sequences to identify cytoplasmic genotypes of onion (Allium cepa L.). Theor Appl Genet 96:367–370

    Article  CAS  Google Scholar 

  • Schweisguth B (1973) Study of a new type of male sterility in onion, Allium cepa L. Ann Amelior Plant 23:221–233

    Google Scholar 

  • Shinjo C (1969) Cytoplasmic-genetic male sterility in cultivated rice, Oryza sativa L. II. The inheritance of male sterility. Jpn J Genet 44:149–156

    Article  Google Scholar 

  • Tsukazaki H, Yamashita K, Yaguchi S, Masuzaki S, Fukuoka H, Yonemaru J, Kanamori H, Kono I, Hang TTM, Shigyo M, Kojima A, Wako T (2008) Construction of SSR-based chromosome map in bunching onion (Allium fistulosum). Theor Appl Genet 117:1213–1223

    Article  CAS  PubMed  Google Scholar 

  • Uyttewaal M, Arnal N, Quadrado M, Martin-Canadell A, Vrielynck N, Hiard S, Bendahman A, Budar F, Mireau H (2008) Characterization of Raphanus sativus pentatricopeptide repeat proteins encoded by the fertility restorer locus for Ogura cytoplasmic male sterility. Plant Cell 20:3331–3345

    Article  CAS  PubMed  Google Scholar 

  • van der Meer QP, van Bennecom JL (1969) Effect of temperature on the occurrence of male sterility in onion (Allium cepa L.). Euphytica 18:389–394

    Article  Google Scholar 

  • Wako T, Tsukazaki H, Ohara T, Yoshida M, Shimazaki S, Ando T, Yamashita K, Kojima A (2009) Verification of variety classification of bunching onion by principal component analysis of morphological traits and growth habit. Bull Natl Inst Veg Tea Sci 8:121–130 (in Japanese)

    Google Scholar 

  • Wang Z, Zou Y, Zhang X, Chen L, Wu H, Su D, Chen Y, Guo J, Luo D, Long Y, Zhong Y, Liu YG (2006) Cytoplasmic male sterility of rice with boro II cytoplasm is caused by a cytotoxic peptide and is restored by two related PPR motif genes via distinct modes of RNA silencing. Plant Cell 18:676–687

    Article  CAS  PubMed  Google Scholar 

  • Yabuno T (1977) Genetic studies on the interspecific cytoplasm substitution lines of Japonica varieties of Oryza sativa L. and O. Glaberrima Steud. Euphytica 26:451–463

    Article  Google Scholar 

  • Yamashita K, Tashiro Y (1999) Possibility of developing male sterile lines of shallot (Allium cepa L. Aggregatum group) with cytoplasm from A. galanthum Kar. et Kir. J Jpn Soc Hortic Sci 68:256–262

    Article  CAS  Google Scholar 

  • Yamashita K, Tashiro Y (2004) Seed productivity test of CMS lines of Japanese bunching onion (Allium fistulosum L.) possessing the cytoplasm of a wild species, A. galanthum Kar. et Kir. Euphytica 136:327–331

    Article  Google Scholar 

  • Yamashita K, Arita H, Tashiro Y (1999a) Cytoplasm of a wild species, Allium galanthum Kar. et Kir., is useful for developing the male sterile line of A. fistulosum L. J Jpn Soc Hortic Sci 68:788–797

    Article  Google Scholar 

  • Yamashita K, Arita H, Tashiro Y (1999b) Isozyme and RAPD markers linked to fertility restoring gene for cytoplasmic male sterile Allium fistulosum L. with cytoplasm of A. galanthum Kar. et Kir. J Jpn Soc Hortic Sci 68:954–959

    Article  CAS  Google Scholar 

  • Yamashita K, Takatori Y, Tashiro Y (2002) Development of sequence characterized amplified region (SCAR) markers linked to the fertility restoring gene for cytoplasmic male sterile Allium fistulosum L. possessing the cytoplasm of A. galanthum Kar. et Kir. J Jpn Soc Hortic Sci 71:777–779

    Article  CAS  Google Scholar 

  • Yamashita K, Takatori Y, Tashiro Y (2005) Chromosomal location of a pollen fertility-restoring gene, Rf, for CMS in Japanese bunching onion (Allium fistulosum L.) possessing the cytoplasm of A. galanthum Kar. et Kir. revealed by genomic in situ hybridization. Theor Appl Genet 111:15–22

    Article  CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. National Institute of Vegetable and Tea Science, National Agriculture and Food Research Organization, Tsu, Mie, 514-2392, Japan

    Ken-ichiro Yamashita, Hikaru Tsukazaki, Akio Kojima, Takayoshi Ohara & Tadayuki Wako

Authors
  1. Ken-ichiro Yamashita
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Hikaru Tsukazaki
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Akio Kojima
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Takayoshi Ohara
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Tadayuki Wako
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Ken-ichiro Yamashita.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Yamashita, Ki., Tsukazaki, H., Kojima, A. et al. Inheritance mode of male sterility in bunching onion (Allium fistulosum L.) accessions. Euphytica 173, 357–367 (2010). https://doi.org/10.1007/s10681-009-0101-7

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10681-009-0101-7

Keywords

Search

Navigation