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Construction of SSR-based chromosome map in bunching onion (Allium fistulosum)

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Abstract

We have constructed a linkage map of bunching onion (Allium fistulosum L., 2n = 16) using an F2 population of 225 plants. The map consists of 17 linkage groups with 212 bunching onion SSR markers and 42 bulb onion (A. cepa L.) SSR, InDel, CAPS or dCAPS markers, covering 2,069 cM. This is the first report of a linkage map mainly based on SSR markers in the genus Allium. With the 103 anchor markers [81 bunching onion SSRs, 11 bulb onion SSRs and 11 bulb onion non-SSRs (1 InDel, 9 CAPSs and 1 dCAPS)] whose chromosome assignments were identified in A. cepa and/or A. fistulosum, via the use of several kinds of Allium alien addition lines, 16 of the 17 linkage groups were connected to the 8 basic chromosomes of A. cepa.

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Acknowledgments

We are grateful to Ms. K. Tanaka, K. Wakita and S. Negoro for the technical assistance. This work was supported by the project “Green Technology Project (DM-1604)”, Ministry of Agriculture, Forestry and Fisheries, Japan and Grant-in-Aid for Young Scientists (B) (19780010), Japan Society for the Promotion of Science. The authors wish to thank Ms. N. Matsubara, Y. Kousabara and M. Anraku (former students in Yamaguchi University) for their contributions to this study.

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Author notes

  1. Shinichi Masuzaki

    Present address: Forensic Science Laboratory, Yamaguchi Pref. Police Headquarters, Yamaguchi, 753-8504, Japan

  2. Junichi Yonemaru

    Present address: QTL Genomics Research Center, National Institute of Agrobiological Sciences (NIAS), 2-1-2 Kannondai, Tsukuba, Ibaraki, 305-8602, Japan

  3. Tran Thi Minh Hang

    Present address: Department of Horticulture, Faculty of Agronomy, Hanoi Agricultural University, Km 10, Nguyen Trai Road, Thanh Xuan District, Hanoi, Vietnam

Authors and Affiliations

  1. National Institute of Vegetable and Tea Science (NIVTS), NARO, 360 Ano-Kusawa, Tsu, Mie, 514-2392, Japan

    Hikaru Tsukazaki, Ken-ichiro Yamashita, Hiroyuki Fukuoka, Akio Kojima & Tadayuki Wako

  2. Department of Biological and Environmental Science, Faculty of Agriculture, Yamaguchi University, 1677-1 Yoshida, Yamaguchi, 753-8515, Japan

    Shigenori Yaguchi, Shinichi Masuzaki, Tran Thi Minh Hang & Masayoshi Shigyo

  3. National Agricultural Research Center for Tohoku Region (NARCT), NARO, 4 Akahira, Shimo-kuriyagawa, Morioka, Iwate, 020-0198, Japan

    Junichi Yonemaru

  4. Institute of the Society for Techno-innovation of Agriculture, Forestry and Fisheries (STAFF), 446-1 Ippaizuka, Kamiyokoba, Tsukuba, Ibaraki, 305-0854, Japan

    Hiroyuki Kanamori & Izumi Kono

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  1. Hikaru Tsukazaki
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  2. Ken-ichiro Yamashita
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  3. Shigenori Yaguchi
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  7. Hiroyuki Kanamori
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  8. Izumi Kono
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  9. Tran Thi Minh Hang
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Corresponding author

Correspondence to Tadayuki Wako.

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Communicated by M. Xu.

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Tsukazaki, H., Yamashita, Ki., Yaguchi, S. et al. Construction of SSR-based chromosome map in bunching onion (Allium fistulosum). Theor Appl Genet 117, 1213–1223 (2008). https://doi.org/10.1007/s00122-008-0849-5

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  • DOI: https://doi.org/10.1007/s00122-008-0849-5

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