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Molecular characterization of Pina and Pinb allelic variations in Xinjiang landraces and commercial wheat cultivars

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Abstract

Grain hardness plays an important role in determining both milling performance and quality of the end-use products produced from common or bread wheat. The objective of this study was to characterize allelic variations at the Pina and Pinb loci in Xinjiang wheat germplasm for further understanding the mechanisms involved in endosperm texture formation, and the status of grain texture in Chinese bread wheat. A total of 291 wheat cultivars, including 56 landraces, and 95 introduced and 140 locally improved cultivars, grown in Xinjiang, were used for SKCS measurement and molecular characterization. Among the harvested grain samples, 185 (63.6%), 40 (13.7%), and 66 (22.7%) were classified as hard, mixed and soft, respectively. Eight different genotypes for the Pina and Pinb loci were identified, including seven previously reported genotypes, viz., Pina-D1a/Pinb-D1a, Pina-D1a/Pinb-D1b, Pina-D1b/Pinb-D1a, Pina-D1a/Pinb-D1p, Pina-D1a/Pinb-D1q, Pina-D1a/Pinb-D1aa, Pina-D1a/Pinb-D1ab, and a novel Pinb allele, Pinb-D1ac. This new allele, detected in Kashibaipi (local landrace) and Red Star (from Russia) has a double mutation at the 257th (G to A substitution) and 382nd (C to T substitution) nucleotide positions of the coding region. Pina-D1b, Pinb-D1b, and Pinb-D1p were the most common alleles in Xinjiang wheat germplasm, with frequencies of 14.3%, 38.1% and 28.6% in hard textured landraces, 25.5%, 56.9% and 11.8% in hard introduced cultivars, and 24.8%, 47.8% and 26.5% in hard locally improved cultivars, respectively. The restriction enzymes ApaI, SapI, BstXI and SfaNI were used to identify Pinb-D1ab or Pinb-D1ac, Pinb-D1b, Pinb-D1e and Pinb-Dg, respectively, by digesting PCR products of the Pinb gene. The unique grain hardness distribution in Xinjiang bread wheat, as well as the CAPs markers for identification of the Pinb alleles provided useful information for breeding wheat cultivars with optimum grain textures.

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Abbreviations

PCR:

Polymerase chain reaction

PINA:

Puroindoline-a protein

Pina :

Gene locus coding for puroindoline-a protein

PINB:

Puroindoline-b protein

Pinb :

Gene locus coding for puroindoline-b protein

SKCS:

Single kernel characterization system

MAS:

Marker-assisted selection

CAP:

Cleavage amplification polymorphism

SNP:

Single nucleotide polymorphism

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Acknowledgments

The authors are grateful to Prof. Bob McIntosh, University of Sydney, for reviewing this manuscript. This project was funded by the Xinjiang Bingtuan Doctoral Foundation (05JC-02), the Taishan Scholarship Program from the Shandong Provincial Government, and the National Basic Research Program (2002CB11300).

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Authors and Affiliations

  1. Institute of Crop Science, National Wheat Improvement Centre/The National Key Facility for Crop Gene Resources and Genetic Improvement, Chinese Academy of Agricultural Sciences (CAAS), 12 Zhongguancun South Street, Beijing, 100081, China

    Liang Wang, Xianchun Xia & Zhonghu He

  2. Institute of Crop Science, Xinjiang Academy of Agri-Reclamation Sciences, Shihezi, Xinjiang, 832000, China

    Liang Wang & Peiyuan Mu

  3. Crop Research Institute, Shandong Academy of Agricultural Sciences, Jinan, Shandong, 250100, China

    Genying Li

  4. International Maize and Wheat Improvement Centre (CIMMYT) China Office, c/o CAAS, 12 Zhongguancun South Street, Beijing, 100081, China

    Zhonghu He

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  1. Liang Wang
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  2. Genying Li
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  3. Xianchun Xia
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  4. Zhonghu He
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Corresponding authors

Correspondence to Zhonghu He or Peiyuan Mu.

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Liang Wang and Genying Li—contributed equally to this work.

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Wang, L., Li, G., Xia, X. et al. Molecular characterization of Pina and Pinb allelic variations in Xinjiang landraces and commercial wheat cultivars. Euphytica 164, 745–752 (2008). https://doi.org/10.1007/s10681-008-9706-5

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  • DOI: https://doi.org/10.1007/s10681-008-9706-5

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