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Mapping of the leaf rust resistance gene Lr38 on wheat chromosome arm 6DL using SSR markers

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Abstract

Leaf rust caused by the fungus Puccinia triticina is one of the most important diseases of wheat (Triticum aestivum) worldwide. The use of resistant wheat cultivars is considered the most economical and environment-friendly approach in controlling the disease. The Lr38 gene, introgressed from Agropyron intermedium, confers a stable seedling and adult plant resistance against multiple isolates tested in Europe. In the present study, 94 F2 plants resulting from a cross made between the resistant Thatcher-derived near-isogenic line (NIL) RL6097, and the susceptible Ethiopian wheat cultivar Kubsa were used to map the Thatcher Lr38 locus in wheat using simple sequence repeat (SSR) markers. Out of 54 markers tested, 15 SSRs were polymorphic between the two parents and subsequently genotyped in the population. The P. triticina isolate DZ7-24 (race FGJTJ), discriminating Lr38 resistant and susceptible plants, was used to inoculate seedlings of the two parents and the segregating population. The SSR markers Xwmc773 and Xbarc273 flanked the Lr38 locus at a distance of 6.1 and 7.9 cM, respectively, to the proximal end of wheat chromosome arm 6DL. The SSR markers Xcfd5 and Xcfd60 both flanked the locus at a distance of 22.1 cM to the distal end of 6DL. In future, these SSR markers can be used by wheat breeders and pathologists for marker assisted selection (MAS) of Lr38-mediated leaf rust resistance in wheat.

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Acknowledgements

The authors thank Dr. Pierre Sourdille (INRA, Clermont Ferrand, France) for providing primer aliquots of SSR markers from set II, Dr. Volker Lind (Federal Center for Breeding Research on Cultivated Plants, BAZ, Quedlinburg, Germany) for provision of seeds of the near-isogenic line RL6097 and Dr. Bedada Girma (Kulumsa Agricultural Research Center, Ethiopia) for providing seeds of cultivar Kubsa. We are also indebted to Ms Hedda von Quistorp for her excellent technical assistance on the SSR analysis.

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  1. S. A. Mebrate

    Present address: Debre Zeit Agricultural Research Center, P.O. Box 32, Debre Zeit, Ethiopia

  2. K. Pillen

    Present address: Max-Planck-Institute for Plant Breeding Research, Barley Genetics Research Group, Carl-von-Linné-Weg 10, 50829, Cologne, Germany

Authors and Affiliations

  1. Institute of Crop Science and Resource Conservation, Phytomedicine, University of Bonn, Nussallee 9, 53115, Bonn, Germany

    S. A. Mebrate, E. C. Oerke & H. W. Dehne

  2. Institute of Crop Science and Resource Conservation, Crop Genetics and Biotechnology, University of Bonn, Katzenburgweg 5, 53115, Bonn, Germany

    K. Pillen

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  1. S. A. Mebrate
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  2. E. C. Oerke
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  3. H. W. Dehne
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Correspondence to K. Pillen.

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Mebrate, S.A., Oerke, E.C., Dehne, H.W. et al. Mapping of the leaf rust resistance gene Lr38 on wheat chromosome arm 6DL using SSR markers. Euphytica 162, 457–466 (2008). https://doi.org/10.1007/s10681-007-9615-z

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