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Allele-specific amplification of polymorphic sites for the detection of powdery mildew resistance loci in cereals

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Abstract

Primers for the polymerase chain reaction (PCR) were tailored to selectively amplify RFLP marker alleles associated with resistance and susceptibility for powdery mildew in cereals. The differentiation between marker alleles for susceptible and resistant genotypes is based on the discrimination of a single nucleotide by using allele-specific oligonucleotides as PCR primers. The PCR assays developed are diagnostic for RFLP alleles at the loci MWG097 in the barley genome and Whs350 in the wheat genome. The first marker locus is closely linked to MlLa resistance in barley, while the latter is linked to Pm2 resistance locus in wheat. PCR analysis of 31 barley and 30 wheat cultivars, with some exceptions, verified the presence or absence of the resistance loci investigated. These rapid PCR-based approaches are proposed as an efficient alternative to conventional procedures for selecting powdery mildew-resistant genotypes in breeding programs.

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References

  • Anonymous (1995) Beschreibende Sortenliste Getreide, Mais, Ölfrüchte, Leguminosen, Hackfrüchte. Bundessortenamt. Landbuchverlag, Hannover

  • D'Ovidio R, Anderson OD (1994) PCR analysis to distinguish between alleles of a member of a multigene family correlated with wheat bread-making quality. Theor Appl Genet 88:759–763

    CAS  Google Scholar 

  • Feuillet C, Messmer M, Schachermayr G, Keller B (1995) Genetic and physical characterization of the Lr1 leaf rust resistance locus in wheat (Triticum aestivum L.). Mol Gen Genet 248:553–562

    CAS  PubMed  Google Scholar 

  • Giese H, Holm-Jensen AG, Jensen HP, Jensen J (1993) Localization of the Laevigatum powdery mildew resistance gene to barley chromosome 2 by the use of RFLP markers. Theor Appl Genet 85:897–900

    Google Scholar 

  • Görg R, Hollricher K, Schulze-Lefert P (1993) Functional analysis and RFLP-mediated mapping of the Mlg resistance locus in barley. Plant J 3:857–866

    Google Scholar 

  • Hartl L, Weiss H, Zeller FJ, Jahoor A (1993) Use of RFLP markers for the identification of alleles of the Pm3 locus conferring powdery mildew resistance in wheat (Triticum aestivum L.). Theor Appl Genet 86:959–963

    Google Scholar 

  • Hartl L, Weiss H, Stephan U, Zeller FJ, Jahoor A (1995) Molecular identification of powdery mildew resistance genes in common wheat (Triticum aestivum L.). Theor Appl Genet 90:601–606

    Google Scholar 

  • Hilbers S, Fischbeck G, Jahoor A (1992) Localization of the Laevigatum resistance gene MlLa against powdery mildew in the barley genome by the use of RFLP markers. Plant Breeding 109: 335–338

    Google Scholar 

  • Hinze K, Thompson RD, Ritter E, Salamini F, Schulze-Lefert P (1991) Restriction fragment length polymorphism-mediated targeting of the ml-o resistance locus in barley (Hordeum vulgare). Proc Natl Acad Sci USA 88:3691–3695

    Google Scholar 

  • Huang M, Arnheim N, Goodman MF (1992) Extension of base mispairs by Taq DNA polymerase: implications for single nucleotide discrimination in PCR. Nucleic Acids Res 20:4567–4573

    Google Scholar 

  • Islam AKRM, Shepherd KW, Sparrow DHB (1981) Isolation and characterization of euplasmic wheat-barley addition lines. Heredity 46:161–174

    Google Scholar 

  • Jensen HP, Christensen E, Jørgensen JH (1992) Powdery mildew resistance genes in 127 northwest european spring barley varieties. Plant Breed 108:210–228

    Google Scholar 

  • Kwok S, Kellogg DE, McKinny N, Spasic D, Goda L, Sninsky JJ (1990) Effects of primer-template mismatches on the polymerase chain reaction: human immunodeficiency virus type-I model studies. Nucleic Acids Res 18:999–1005

    CAS  PubMed  Google Scholar 

  • Ma ZQ, Sorrells ME, Tanksley SD (1994) RFLP markers linked to powdery mildew resistance genes Pm1, Pm2, Pm3 and Pm4 in wheat. Genome 37:871–875

    Google Scholar 

  • Niewöhner J, Salamini F, Gebhardt C (1995) Development of a PCR assay diagnostic for RFLP marker alleles closely linked to alleles Gro1 and H1, conferring resistance to the root cyst nematode Globodera rostochiensis potato. Mol Breed 1:65–78

    Google Scholar 

  • Olson M, Hood L, Cantor CH, Botstein D (1989) A common language for the physical mapping of the human genome. Science 24:1434–1435

    Google Scholar 

  • Röder MS, Plaschke J, König SU, Börner A, Sorrells ME, Tanksley SD, Ganal MW (1995) Abundance, variability and chromosomal location of microsatellites in wheat. Mol Gen Genet 246:327–333

    PubMed  Google Scholar 

  • Schachermayr G, Siedler H, Gale MD, Winzeler H, Winzeler M, Keller B (1994) Identification and localization of molecular markers linked to the Lr9 leaf rust resistance gene of wheat. Theor Appl Genet 88:110–115

    Google Scholar 

  • Schachermayr GM, Messmer MM, Feuillet C, Winzeler H, Winzeler M, Keller B (1995) Identification of molecular markers linked to the Agropyron elongatum-derived leaf rust resistance gene Lr24 in wheat. Theor Appl Genet 90:982–990

    Google Scholar 

  • Schönfeld M, Ragni A, Fischbeck G, Jahoor A (1996) RFLP mapping of three new loci for resistance genes to powdery mildew (Erysiphe graminis f. sp. hordei) in barley. Theor Appl Genet 93:48–56

    Article  Google Scholar 

  • Schüller C, Backes G, Fischbeck G, Jahoor A (1992) RFLP markers to identify the alleles on the Mla locus conferring powdery mildew resistance in barley. Theor Appl Genet 84:330–338

    Google Scholar 

  • Sears ER (1966) Nullisomic-tetrasomic combinations in hexaploid wheat. In: Riley R, Lewis KR (eds) Chromosome manipulation and plant genetics. Oliver and Boyd, Edinburgh, pp 29–45

    Google Scholar 

  • Shattuck-Eidens DM, Bell RN, Mitchell JT, McWorther VC (1991) Rapid detection of maize DNA sequence variation. GATA 8:240–245

    Google Scholar 

  • Talbert LE, Blake NK, Chee PW, Blake TK, Magyar GM (1994) Evaluation of ‘sequence-tagged-site’ PCR products as molecular markers in wheat. Theor Appl Genet 87:789–794

    CAS  Google Scholar 

  • Tragoonrung S, Kanazin V, Hayes PM, Blake TK (1992) Sequencetagged-site-facilitated PCR for barley genome mapping. Theor Appl Genet 84:1002–1008

    Google Scholar 

  • Williams GK, Kubelik AR, Kenneth JL, Rafalski A, Scott VT (1991) DNA polymorphism amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Res 18:6531–6535

    Google Scholar 

  • Xu L, Hall BG (1994) SASA: a simplified, reliable method for allele-specific amplification of polymorphic sites. BioTechniques 6:44–45

    Google Scholar 

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Authors and Affiliations

  1. Lehrstuhl für Pflanzenbau und Pflanzenzüchtung, Technische Universität München, 85350, Freising-Weihenstephan, Germany

    V. Mohler & A. Jahoor

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  1. V. Mohler
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  2. A. Jahoor
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Communicated by J. W. Snape

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Mohler, V., Jahoor, A. Allele-specific amplification of polymorphic sites for the detection of powdery mildew resistance loci in cereals. Theoret. Appl. Genetics 93, 1078–1082 (1996). https://doi.org/10.1007/BF00230128

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  • DOI: https://doi.org/10.1007/BF00230128

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