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The CMS-associated 16 kDa protein encoded by orfH522 in the PET1 cytoplasm is also present in other male-sterile cytoplasms of sunflower

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Abstract

In sunflower plants carrying the PET1 cytoplasm male sterility (CMS) is associated with a new open reading frame (orfH522) in the 3′-flanking region of the atpA gene and an additional 16 kDa protein. Twenty-seven male-sterile cytoplasms of different origin were studied for the expression of the 16 kDa protein. In addition to the PET1 cytoplasm nine other male-sterile cytoplasms express the CMS-associated protein. These CMS sources originate from different interspecific crosses, from spontaneously occurring male-sterile plants in wild sunflower and from induced mutagenesis. Polyclonal antisera were raised against fusion proteins which contain 421 bp of the 3′-coding region of orfH522 to verify by immunological methods the identity of the protein in the other CMS cytoplasms. The anti-ORFH522 antiserum showed a positive reaction in the immunoblot with all CMS cytoplasms expressing the 16 kDa protein. Investigations of the mitochondrial DNA demonstrated that all ten CMS cytoplasms which express the 16 kDa protein have the same organization at the atpA locus. OrfH522 is located in the 3′-flanking region of the atpA gene. Transcript analyses using atpA and orfH522 as probes gave the same transcript pattern for the investigated CMS cytoplasms, just as for PET1. The MAX1 cytoplasm has an orfH522-related sequence but does not synthesize the 16 kDa protein. Using the sodium carbonate treatment the 16 kDa protein proved to be membrane-bound. Computer analyses predict that the hydrophobic N-terminal region of ORFH522 may form a transmembrane helix functioning as membrane anchor.

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Authors and Affiliations

  1. Institut für Pflanzenbau und Pflanzenzüchtung I, Justus-Liebig-Universität, Ludwigstrasse 23, D-35390, Giessen, Germany

    Renate Horn & Wolfgang Friedt

  2. Institut für Pflanzenphysiologie, Justus-Liebig-Universität, Heinrich-Buff-Ring 54-62, D-35392, Giessen, Germany

    Joachim E. G. Hustedt, Andreas Horstmeyer & Klaus Zetsche

  3. Institut für Mikro- und Molekularbiologie, Justus-Liebig-Universität, Frankfurter Strasse 107, D-35392, Giessen, Germany

    Josef Hahnen

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  1. Renate Horn
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  2. Joachim E. G. Hustedt
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  3. Andreas Horstmeyer
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  4. Josef Hahnen
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  5. Klaus Zetsche
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  6. Wolfgang Friedt
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Horn, R., Hustedt, J.E.G., Horstmeyer, A. et al. The CMS-associated 16 kDa protein encoded by orfH522 in the PET1 cytoplasm is also present in other male-sterile cytoplasms of sunflower. Plant Mol Biol 30, 523–538 (1996). https://doi.org/10.1007/BF00049329

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  • DOI: https://doi.org/10.1007/BF00049329

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