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First report of a genetic map and evidence of QTL for resistance to CABMV in a segregating population of Passiflora

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Abstract

The fruit woodiness disease induced by the cowpea aphid-borne mosaic virus (CABMV) is considered to be of the greatest economic importance in the Passiflora crop. There are no reports of resistance to CABMV identified in P. edulis, and none of the Passiflora cultivars registered thus far are resistant to the virus. On this basis, breeding programs have resorted to wild species to identify sources of resistance that can be efficiently transferred to the cultivated species via interspecific crossing. A preliminary map for a segregating population of Passiflora based on seven microsatellite markers and 43 inter-simple sequence repeat markers was constructed using a BC1 population composed of 187 individuals. The map was generated using JoinMap software and the linkage groups were formed and ranked using a lod score of 3.0 and a maximum recombination value of 40%. The linkage map consisted of 50 markers - 43 ISSR and seven SSR. The generated map covered 1017.1 cM, with one larger linkage group of 211.2 cM and eight smaller groups ranging from 1.8–179.3 cM. Each linkage group contained 3–12 markers, with one marker occurring at every 20.34 cM. Of the total markers, 55% were mapped, and 40.68% of the Passiflora map was covered. Seven small-effect QTL were detected for resistance to CABMV in seven linkage groups. The phenotypic variation rate ranged from 1.45 to 4.68%, totaling 21.81%. This is the first report involving QTL mapping for resistance to CABMV in a segregating population of Passiflora obtained from interspecific crossing.

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Authors and Affiliations

  1. Universidade Estadual do Norte Fluminense Darcy Ribeiro (UENF), Center of Agricultural Sciences and Technology, Av. Alberto Lamego, 2000, Parque Califórnia, Campos dos Goytacazes, RJ, 28013-600, Brazil

    Eileen Azevedo Santos, Alexandre Pio Viana, Fernando Henrique de Barros Walter, Helaine Christine Cancela Ramos & Marcela Santana Bastos Boechat

  2. Universidade Estadual de Goiás (UEG) - Posse Campus, Av. Sra. de Santana, 598 - St. Santa Luzia, Posse, GO, 73900-000, Brazil

    Josie Cloviane de Oliveira Freitas

Authors
  1. Eileen Azevedo Santos
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  2. Alexandre Pio Viana
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  3. Fernando Henrique de Barros Walter
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  4. Josie Cloviane de Oliveira Freitas
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  5. Helaine Christine Cancela Ramos
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  6. Marcela Santana Bastos Boechat
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Correspondence to Eileen Azevedo Santos.

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The authors declare that they have no conflict of interest and certify that this work was carried out in a public research organization and that no potential source of conflict of interest exists with any other public or private research organization. This article does not contain any studies with human participants or animals performed by any of the authors.

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Santos, E.A., Viana, A.P., de Barros Walter, F.H. et al. First report of a genetic map and evidence of QTL for resistance to CABMV in a segregating population of Passiflora. Eur J Plant Pathol 155, 903–915 (2019). https://doi.org/10.1007/s10658-019-01822-y

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