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ISSR Markers as a Tool for the Assessment of Genetic Diversity in Passiflora

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Abstract

Genetic variation among sweet, purple, and yellow passion fruit accessions was assessed using inter-simple sequence repeat (ISSR) markers. Eighteen ISSR primers were used to evaluate 45 accessions. The number of polymorphic bands per primer varied from 4 to 22, with 12.4 bands per primer on average. Nei’s genetic distance between accessions ranged from 0.04 to 0.35. Clustering using the neighbor-joining method resulted in the formation of 11 major clusters. It was not possible to classify the accessions according to their geographic origin, showing that there is no structure in the gene bank. The overall mean Shannon–Weaver diversity index was 0.32, indicating good resolution of genetic diversity in passion fruit germplasm using ISSR markers. Our results indicate that ISSR can be useful for genetic diversity studies, to provide practical information for parental selection and to assist breeding and conservation strategies.

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Acknowledgments

This work was supported by Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) and Fundação de Apoio à Pesquisa do Estado da Bahia (Fapesb).

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Authors and Affiliations

  1. Universidade Federal do Recôncavo da Bahia, Cruz das Almas, BA, Brazil

    Lucas Ferraz dos Santos & Aline dos Santos Silva

  2. Embrapa Mandioca e Fruticultura Tropical, Cruz das Almas, BA, 44380-000, Brazil

    Eder Jorge de Oliveira & Juliana Leles Costa

  3. Faculdade Maria Milza, Cruz das Almas, BA, Brazil

    Fabiana Moraes de Carvalho

  4. Embrapa Recursos Genéticos e Biotecnologia, Brasília, DF, Brazil

    Juliano Gomes Pádua

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  1. Lucas Ferraz dos Santos
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Correspondence to Eder Jorge de Oliveira.

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dos Santos, L.F., de Oliveira, E.J., dos Santos Silva, A. et al. ISSR Markers as a Tool for the Assessment of Genetic Diversity in Passiflora . Biochem Genet 49, 540–554 (2011). https://doi.org/10.1007/s10528-011-9429-5

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