Skip to main content
SpringerLink
Log in

Induction of Systemic Resistance Against Bacterial Wilt in Eucalyptus urophylla by Fluorescent Pseudomonas spp

  • Published:
European Journal of Plant Pathology Aims and scope Submit manuscript

Abstract

The ability of selected strains of fluorescent Pseudomonas spp. to cause induced systemic resistance (ISR) in Eucalyptus urophylla against bacterial wilt caused by Ralstonia solanacearum was investigated. Four of the five strains used can produce salicylic acid (SA) in vitro and, therefore, chemical SA, that is known to induce resistance in many plant species, was used as a reference treatment. Whereas a soil drench with SA did induce systemic resistance in E. urophylla, infiltration of SA into leaves did not. None of the fluorescent Pseudomonas spp. strains caused ISR against bacterial wilt when applied to the soil, but two strains, P. putida WCS358r and P. fluorescens WCS374r triggered ISR when infiltrated into two lower leaves 3–7 days before challenge inoculation. A mutant of strain WCS358r defective in the biosynthesis of the fluorescent siderophore pseudobactin, did not cause ISR, while the purified siderophore of WCS358r did, suggesting that pseudobactin358 is the ISR determinant of WCS358. A siderophore-minus mutant of WCS374r induced the same level of disease resistance as its parental strain, but the purified siderophore induced resistance as well, indicating that both the siderophore and another, unknown, inducing determinant(s) of WCS374r can trigger ISR in Eucalyptus. A possible role of WCS374r-produced SA remains uncertain. Transformation of a siderophore-minus mutant of WCS358 with the SA biosynthetic gene cluster from WCS374 did not enable this transformant to cause ISR in E. urophylla.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • K Audenaert T Pattery P Cornelis M Höfte (2002) ArticleTitleIn duction of systemic resistance to Botrytis cinerea in tomato by Pseudomonas aeruginosa 7NSK2: role of salicylic acid, pyochelin, and pyocyanin Molecular Plant–Microbe Interactions 15 1147–1156 Occurrence Handle12423020 Occurrence Handle1:CAS:528:DC%2BD38XosVGru7Y%3D

    PubMed  CAS  Google Scholar 

  • PAHM Bakker LX Ran CMJ Pieterse LC Loon ParticleVan (2003) ArticleTitleUnderstanding the involvement of rhizobacteria-mediated induction of systemic resistance in biocontrol of plant diseases Canadian Journal of Plant Pathology 25 5–9 Occurrence Handle10.1080/07060660309507043

    Article  Google Scholar 

  • J Bigirimana M Höfte (2002) ArticleTitleInduction of systemic resistance to Colletotrichum lindemuthianum in bean by a benzothiadiazole derivative and rhizobacteria Phytoparasitica 30 159–168

    Google Scholar 

  • S Buysens K Huengens J Poppe M Höfte (1996) ArticleTitleInvolvement of pyochelin and pyoverdin in suppression of Pythium-induced damping-off of tomato by Pseudomonas aeruginosa 7NSK2 Applied and Environmental Microbiology 62 865–871 Occurrence Handle1:CAS:528:DyaK28XhsVWqs7s%3D Occurrence Handle16535275

    CAS  PubMed  Google Scholar 

  • G Meyer ParticleDe M Höfte (1997) ArticleTitleSalicylic acid produced by the rhizobacterium Pseudomonas aeruginosa 7NSK2 induces resistance to leaf infection by Botrytis cinerea on bean Phytopathology 87 588–593 Occurrence Handle18945074

    PubMed  Google Scholar 

  • G Meyer ParticleDe K Audenaert M Höfte (1999a) ArticleTitlePseudomonas aeruginosa 7NSK2 induced systemic resistance in tobacco depends on in planta salicylic acid accumulation but is not associated with PR1a expression European Journal of Plant Pathology 105 513–517

    Google Scholar 

  • G Meyer ParticleDe K Capieau K Audenaert A Buchala JP Métraux M Höfte (1999b) ArticleTitleNanogram amounts of salicylic acid produced by the rhizobacterium Pseudomonas aeruginosa 7NSK2 activate the systemic acquired resistance pathway in bean Molecular Plant–Microbe Interactions 12 450–458

    Google Scholar 

  • Défago G, Berling CH, Burger U, Haas D, Kahr G, Keel C, Voisard C, Wirthner P and Wuthrich B (1990). Suppression of black root rot of tobacco and other root diseases by strains of Pseudomonas fluorescens: Potential applications and mechanisms. In: Hornby D, Cook RJ, Henis Y, Ko WH, Rovira AD, Schippers B and Scott PR (eds)Biological Control of Soilborne Plant Pathogens (pp. 93–108) CAB International.

  • BJ Duijff JW Meijer PAHM Bakker B Schippers (1993) ArticleTitleSiderophore mediated competition for iron and induced resistance in the suppression of fusarium wilt of carnation by fluorescent Pseudomonas spp. Netherlands Journal of Plant Pathology 99 277–289 Occurrence Handle1:CAS:528:DyaK2cXivFCgtLg%3D

    CAS  Google Scholar 

  • BJ Duijff PAHM Bakker B Schippers (1994) ArticleTitleSuppression of fusarium wilt of carnation by Pseudomonas putida WCS358 at different levels of disease incidence and iron availability Biocontrol Science and Technology 4 279–288

    Google Scholar 

  • BJ Duijff D Pouhair C Olivain C Alabouvette P Lemanceau (1998) ArticleTitleImplication of systemic induced resistance in the suppression of fusarium wilt of tomato by Pseudomonas fluorescens WCS417r and by nonpathogenic Fusarium oxysporum Fo47 European Journal of Plant Pathology 104 903–910 Occurrence Handle10.1023/A:1008626212305

    Article  Google Scholar 

  • PC Fahy AC Hayward (1983) Media and methods for isolation and diagnostic tests PC Fahy GJ Persley (Eds) Plant Bacterial Diseases–A Diagnostic Guide Academic Press Sydney 337–378

    Google Scholar 

  • SM Gan JY Bai KM Wu JY Wu JM Xu (1998) ArticleTitleSeedling resistance of interspecific crosses from female Eucalyptus urophylla to bacterial wilt Forest Research 11 569–573

    Google Scholar 

  • FP Geels B Schippers (1983) ArticleTitleSelection of antagonistic flurorescent Pseudomonas spp and their root colonization and persistence following treatment of seed potatoes Phytopathologische Zeitschrift 108 193–206

    Google Scholar 

  • DR Hoagland DI Arnon (1938) ArticleTitleThe water culture method for growing plants without soil California Agricultural Experiment Station Bulletin 347 36–39

    Google Scholar 

  • A Iavicoli E Boutet A Buchala JP Métraux (2003) ArticleTitleInduced systemic resistance in Arabidopsis thaliana in response to root inoculation with Pseudomonas fluorescens CHA0 Molecular Plant–Microbe Interactions 16 851–858 Occurrence Handle14558686 Occurrence Handle1:CAS:528:DC%2BD3sXnsFyktLs%3D Occurrence Handle10.1094/MPMI.2003.16.10.851

    Article  PubMed  CAS  Google Scholar 

  • LF Johnson E Curl (1972) Testing for antibiotic activity LF Johnson E Curl (Eds) Methods for Research on the Ecology of Soil-borne Plant Pathogens Burgess Publishing Company Minnesota 153–166

    Google Scholar 

  • C Keel U Schnider M Maurhofer C Voisard J Laville U Burger P Wirthner D Haas G Défago (1992) ArticleTitleSuppression of root diseases by Pseudomonas fluorescens CHA0: Importance of the bacterial secondary metabolite 2,4-diacetylphloroglucinol Molecular Plant–Microbe Interactions 5 4–13 Occurrence Handle1:CAS:528:DyaK38Xht1GjtLY%3D

    CAS  Google Scholar 

  • A Kelman (1954) ArticleTitleThe relationship of pathogenicity in Pseudomonas solanacearum to colony appearance on a tetrazolium medium Phytopathology 44 683–685

    Google Scholar 

  • EO King MK Ward DE Raney (1954) ArticleTitleTwo simple media for the demonstration of pyocyanin and fluorescin Journal of Laboratory and Clinical Medicine 44 301–307 Occurrence Handle13184240 Occurrence Handle1:STN:280:DyaG2c7gsVCisQ%3D%3D

    PubMed  CAS  Google Scholar 

  • JW Kloepper S Tuzun JA Kuc (1992) ArticleTitleProposed definitions related to induced disease resistance Biocontrol Science and Technology 2 349–351 Occurrence Handle10.1080/09583159209355251

    Article  Google Scholar 

  • JG Lamers B Schippers FP Geels (1988) Soil-borne diseases of wheat in the Netherlands and results of seed bacterization with pseudomonads against Gaeumannomyces graminis var. tritici, associated with disease resistance ML Jorna LAJ Slootmaker (Eds) Cereal Breeding Related to Integrated Cereal Production Pudoc Wageningen 134–139

    Google Scholar 

  • M Leeman JA Pelt ParticleVan FM Ouden ParticleDen M Heinsbroek PAHM Bakker B Schippers (1995a) ArticleTitleInduction of systemic resistance against fusarium wilt of radish by lipopolysaccharides of Pseudomonas fluorescens Phytopathology 85 1021–1027 Occurrence Handle1:CAS:528:DyaK2MXptV2ksL8%3D

    CAS  Google Scholar 

  • M Leeman JA Pelt ParticleVan FM Ouden ParticleDen M Heinsbroek PAHM Bakker B Schippers (1995b) ArticleTitleInduction of systemic resistance by Pseudomonas fluorescens in radish cultivars differing in susceptibility to fusarium wilt, using a novel bioassay European Journal of Plant Pathology 101 655–664 Occurrence Handle10.1007/BF01874869

    Article  Google Scholar 

  • M Leeman EM Ouden ParticleDen JA Pelt ParticleVan FPM Dirkx H Steijl PAHM Bakker B Schippers (1996) ArticleTitleIron availability affects induction of systemic resistance to fusarium wilt of radish by Pseudomonas fluorescens Phytopathology 86 149–155 Occurrence Handle1:CAS:528:DyaK28Xhs1emsLk%3D

    CAS  Google Scholar 

  • XP Lin XJ Lin GJ Wu XX Li JX Cai SC Xu (1996) ArticleTitleThe epidemic law of bacterial wilt in Eucalyptus Journal of Central South Forestry University 16 49–55

    Google Scholar 

  • YH Loo (1945) ArticleTitleAssay of streptomycin by the paper-disc plate method Journal of Bacteriology 50 701–709 Occurrence Handle1:CAS:528:DyaH28XhtlWnsg%3D%3D Occurrence Handle16561049

    CAS  PubMed  Google Scholar 

  • XM Lu JH Pan (1995) ArticleTitleInvestigation of diseases and pests of Eucalyptus in Liuzhou, China Guangxi Plant Protection 2 17–18

    Google Scholar 

  • JD Marugg M Spanje ParticleVan WPM Hoekstra B Schippers PJ Weisbeek (1985) ArticleTitleIsolation and analysis of genes involved in siderophore biosynthesis in plant growth-stimulating Pseudomonas putida WCS358 Journal of Bacteriology 164 563–570 Occurrence Handle2997118 Occurrence Handle1:CAS:528:DyaL28XhsV2k

    PubMed  CAS  Google Scholar 

  • M Maurhofer C Hase P Meuwly JP Métraux G Défago (1994) ArticleTitleInduction of systemic resistance of tobacco to tobacco necrosis virus by the root-colonizing Pseudomonas fluorescens strain CHA0: Influence of the gacA gene and of pyoverdine production Phytopathology 84 139–146 Occurrence Handle1:CAS:528:DyaK2cXjtF2ls78%3D

    CAS  Google Scholar 

  • J Mercado-Blanco KMGM Drift ParticleVan der PE Olsson JE Thomas-Oates LC Loon ParticleVan PAHM Bakker (2001) ArticleTitleAnalysis of the pmsCEAB gene cluster involved in biosynthesis of salicylic acid and the siderophore pseudomonine in the biocontrol strain Pseudomonas fluorescens WCS374 Journal of Bacteriology 183 1909–1920 Occurrence Handle10.1128/JB.183.6.1909-1920.2001 Occurrence Handle11222588 Occurrence Handle1:CAS:528:DC%2BD3MXhvVeksL0%3D

    Article  PubMed  CAS  Google Scholar 

  • H Meziane I Sluis ParticleVan der LC Loon ParticleVan M Höfte PAHM Bakker (2005) ArticleTitleDeterminants of Pseudomonas putida WCS358 involved in inducing systemic resistance in plants Molecular Plant Pathology 6 177–185 Occurrence Handle10.1111/j.1364-3703.2005.00276.x Occurrence Handle20565648

    Article  PubMed  Google Scholar 

  • CMJ Pieterse SCM Wees ParticleVan E Hoffland JA Pelt ParticleVan LC Loon ParticleVan (1996) ArticleTitleSystemic resistance in Arabidopsis induced by biocontrol bacteria is independent of salicylic acid accumulation and pathogenesis-related gene expression Plant Cell 8 1225–1237 Occurrence Handle10.1105/tpc.8.8.1225 Occurrence Handle8776893 Occurrence Handle1:CAS:528:DyaK28XltlKjsbc%3D

    Article  PubMed  CAS  Google Scholar 

  • JM Raaijmakers W Bitter HLM Punte PAHM Bakker PJ Weisbeek B Schippers (1994) ArticleTitleSiderophore receptor pupa as a marker to monitor wild-type Pseudomonas putida WCS358 in natural environments Applied and Environmental Microbiology 60 1184–1190 Occurrence Handle8017914 Occurrence Handle1:CAS:528:DyaK2cXis1Glurk%3D

    PubMed  CAS  Google Scholar 

  • JM Raaijmakers M Leeman MMP oorschot Particlevan I sluis ParticleVan der B Schippers PAHM Bakker (1995) ArticleTitleDose-response relationships in biological control of fusarium wilt of radish by Pseudomonas spp Phytopathology 85 1075–1081

    Google Scholar 

  • LX Ran CY Liu GJ Wu LC Loon ParticleVan PAHM Bakker (2005) ArticleTitleSuppression of bacterial wilt in Eucalyptus urophylla by fluorescent Pseudomonas spp. in China Biological Control 32 111–120 Occurrence Handle10.1016/j.biocontrol.2004.08.007

    Article  Google Scholar 

  • Ran LX, Van Loon LC, Bakker PAHM, (2000) Effects of temperature and iron availability on induction of systemic resistance by salicylic acid producing fluorescent Pseudomonas bacteria. Auburn University website, available: http://www.ag.auburn.edu/argentina/pdfmanuscripts/ran.pdf [Accessed, 22/10/2004].

  • L Sticher B Mauch-Mani JP Metraux (1997) ArticleTitleSystemic acquired resistance Annual Review of Phytopathology 35 235–270 Occurrence Handle10.1146/annurev.phyto.35.1.235 Occurrence Handle15012523 Occurrence Handle1:CAS:528:DyaK2sXmtVGhsrg%3D

    Article  PubMed  CAS  Google Scholar 

  • EW Stutz G Défago H Kern (1986) ArticleTitleNaturally occuring fluorescent pseudomonads involved in suppression of black root rot of tobacco Phytopathology 76 181–185

    Google Scholar 

  • LC Loon ParticleVan JF Antoniw (1982) ArticleTitleComparison of the effects of salicylic acid and ethephon with virus-induced hypersensitivity and acquired resistance in tobacco Netherlands Journal of Plant Pathology 88 237–256 Occurrence Handle10.1007/BF02000130

    Article  Google Scholar 

  • LC Loon ParticleVan PAHM Bakker CMJ Pieterse (1998) ArticleTitleSystemic resistance induced by rhizosphere bacteria Annual Review of Phytopathology 36 453–483 Occurrence Handle15012509

    PubMed  Google Scholar 

  • R Peer ParticleVan GJ Niemann B Schippers (1991) ArticleTitleInduced resistance and phytoalexin accumulation in biological control of fusarium wilt of carnation by Pseudomonas sp. strain WCS417r Phytopathology 81 728–734

    Google Scholar 

  • R Peer ParticleVan B Schippers (1992) ArticleTitleLipopolysaccharides of plant growth promoting Pseudomonas sp. strain WCS417r induce resistance in carnation to fusarium wilt Netherlands Journal of Plant Pathology 98 129–139 Occurrence Handle10.1007/BF01996325

    Article  Google Scholar 

  • SCM Wees ParticleVan CMJ Pieterse A Trijssenaar YAM Van’t Westende F Hartog LC Loon ParticleVan (1997) ArticleTitleDifferential induction of systemic resistance in Arabidopsis by biocontrol bacteria Molecular Plant–Microbe Interactions 10 716–724 Occurrence Handle9245833

    PubMed  Google Scholar 

  • G Wei JW Kloepper S Tuzun (1991) ArticleTitleInduction of systemic resistance of cucumber to Colletotrichum orbiculare by select strains of plant growth-promoting rhizobacteria Phytopathology 81 1508–1512

    Google Scholar 

  • PJ Weisbeek GAJM Hofstad ParticleVan der B Schippers JD Marugg (1986) Genetic analysis of the iron-uptake system of two plant growth-promoting Pseudomonas strains TR Swinburne (Eds) Iron, Siderophores and Plant Diseases Plenum Press New York 299–313

    Google Scholar 

  • QP Wu ZC Liang (1988) ArticleTitleSelection of species and provenances of Eucalyptus for resistance to bacterial wilt Journal of South China Agricultural University 9 41–46

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Faculty of Biology, Section Phytopathology, Utrecht University, 80084, 3508TB, Utrecht, The Netherlands

    L. X. Ran, L. C. van Loon & P. A H. M. Bakker

  2. Section Forest Pathology, Central South Forestry College, 412006, Zhuzhou, Hunan, P.R. China

    L. X. Ran, Z. N. Li & G. J. Wu

  3. Section Forest Pathology, Forestry College, Agricultural University of Hebei, 071001, Baoding, Hebei, P.R. China

    L. X. Ran

Authors
  1. L. X. Ran
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Z. N. Li
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. G. J. Wu
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. L. C. van Loon
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. P. A H. M. Bakker
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to P. A H. M. Bakker.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Ran, L.X., Li, Z.N., Wu, G.J. et al. Induction of Systemic Resistance Against Bacterial Wilt in Eucalyptus urophylla by Fluorescent Pseudomonas spp. Eur J Plant Pathol 113, 59–70 (2005). https://doi.org/10.1007/s10658-005-0623-3

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10658-005-0623-3

Keywords

Search

Navigation