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Induction of systemic resistance byPseudomonas fluorescens in radish cultivars differing in susceptibility to fusarium wilt, using a novel bioassay

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Abstract

Pseudomonas fluorescens-mediated induction of systemic resistance in radish against fusarium wilt (Fusarium oxysporum f. sp.raphani) was studied in a newly developed bioassay using a rockwool system. In this bioassay the pathogen and bacterium were confirmed to be confined to spatially separate locations on the plant root, throughout the experiment. Pathogen inoculum obtained by mixing peat with microconidia and subsequent incubation for four days at 22 °C, yielded a better percentage of diseased plants than a microconidial suspension drench, an injection of a microconidial suspension into the hypocotyl, or a talcum inoculum.Pseudomonas fluorescens strain WCS374 applied in talcum or peat, but not as a suspension drench, induced systemic resistance. A minimal initial bacterial inoculum density of ≥105 CFU WCS374 root−1 was required to significantly reduce the percentage diseased plants. At least one day was necessary between bacterization of strain WCS374 in talcum on the root tips and inoculation of the pathogen in peat on the root base, for an optimal induction of systemic resistance. Strain WCS374 induced systemic resistance in six radish cultivars differing in their susceptibility toF. oxysporum f. sp.raphani. Significant suppression of disease by bacterial treatments was generally observed when disease incidence in the control treatment, depending on pathogen inoculum density, ranged between approximately 40 to 80%. Strains WCS374 and WCS417 ofPseudomonas fluorescens induced systemic resistance against fusarium wilt, whereasP. putida WCS358 did not. This suggests that the induction of systemic resistance byPseudomonas spp. is dependent on strain-specific traits.

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Abbreviations

CFU:

colony forming units

IFC:

immunofluorescence colony-staining

ISR:

induced systemic resistance

PBS:

phosphate buffered saline

SAR:

systemic acquired resistance

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Authors and Affiliations

  1. Department of Plant Ecology and Evolutionary Biology, Section of Plant Pathology, Utrecht University, P.O. Box 800.84, 3508 TB, Utrecht, The Netherlands

    M. Leeman, J. A. van Pelt, F. M. den Ouden, M. Heinsbroek, P. A. H. M. Bakker & B. Schippers

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  1. M. Leeman
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  2. J. A. van Pelt
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  3. F. M. den Ouden
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  4. M. Heinsbroek
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  5. P. A. H. M. Bakker
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  6. B. Schippers
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Leeman, M., van Pelt, J.A., den Ouden, F.M. et al. Induction of systemic resistance byPseudomonas fluorescens in radish cultivars differing in susceptibility to fusarium wilt, using a novel bioassay. Eur J Plant Pathol 101, 655–664 (1995). https://doi.org/10.1007/BF01874869

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