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Foliar application of Zinc oxide nanoparticles alleviates cadmium toxicity in purslane by maintaining nutrients homeostasis and improving the activity of antioxidant enzymes and glyoxalase system

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Abstract

Cadmium (Cd) reduces plant growth by interfering with important plant metabolic processes at the physiological, biochemical, and molecular levels. Here, the effects of foliar application of zinc oxide nanoparticles (ZnO-NPs) on growth, antioxidant enzymes, glyoxalase system, and macro- and micro-elements levels of purslane (portulaca oleracea L.) under Cd toxicity were investigated. The results revealed that Cd toxicity increased the levels of hydrogen peroxide (H2O2), methylglyoxal (MG) and malondialdehyde (MDA), resulting in oxidative stress and the induction of electrolyte leakage (EL). Cd stress enhanced the leaf concentration of Cd and declined the leaf concentrations of macro- and micro-elements, resulting in a decrease in the content of photosynthetic pigments and plant growth. However, the foliar application of ZnO-NPs improved the activity of antioxidant enzymes and the glyoxalase system and, consequently, reduced the levels of H2O2, MG, MDA, and EL in Cd-stressed plants. ZnO-NPs decreased the leaf concentration of Cd and restored the leaf concentrations of macro- and micro-elements, thereby improving photosynthetic pigments and the growth of Cd-stressed purslane plants. In general, the results revealed that the foliar application of ZnO-NPs improved the growth of purslane plants under Cd phytotoxicity by maintaining nutrient homeostasis, improving the defense mechanisms (antioxidant enzymes and glyoxalase cycle), and increasing the accumulation of proline and glutathione. Therefore, the results of the present study strongly recommend that ZnO-NPs could be used effectively in the cultivation of plants in areas contaminated with toxic Cd metal.

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Authors and Affiliations

  1. Department of Biology, Islamshahr Branch, Islamic Azad University, Islamshahr, Iran

    Leila Pishkar & Soheil Yousefi

  2. Department of Biology, Science and Research Branch, Islamic Azad University, Teheran, Iran

    Alireza Iranbakhsh

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  1. Leila Pishkar
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  2. Soheil Yousefi
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  3. Alireza Iranbakhsh
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SY and LP conceived the idea and wrote the manuscript. LP and AI corrected the language of the manuscript. SY and LP conducted the literature survey.

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Correspondence to Leila Pishkar.

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Pishkar, L., Yousefi, S. & Iranbakhsh, A. Foliar application of Zinc oxide nanoparticles alleviates cadmium toxicity in purslane by maintaining nutrients homeostasis and improving the activity of antioxidant enzymes and glyoxalase system. Ecotoxicology 31, 667–678 (2022). https://doi.org/10.1007/s10646-022-02533-7

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