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Dextran Sulfate Sodium-Induced Colitis Is Associated with Enhanced Low Molecular Mass Polypeptide 2 (LMP2) Expression and Is Attenuated in LMP2 Knockout Mice

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Abstract

Low molecular mass polypeptide 2 (LMP2) is an inducible proteasome subunit. Our goals were to examine LMP2 expression in mice with dextran sulfate sodium (DSS)-induced colitis and to evaluate colitis in LMP2 knockout (LMP2–/–) mice. Mice were given 2.5% DSS in the drinking water. On day 0, 2, 4, or 6 after DSS treatment, LMP2 expression was determined in the distal colon by western blot and immunohistochemistry. Parameters of colitis were measured in LMP2–/– mice or wild-type mice. LMP2 expression was enhanced in the colon of DSS-treated mice at all time points. Symptoms of DSS-induced colitis were always lower in LMP2–/– mice. Normalized histology scores and colonic IL-1ß levels increased over the 6-day study period in wild-type mice. These parameters were significantly reduced in LMP2–/– mice that consumed DSS for 6 days. Enhanced LMP2 expression contributes to the pathogenesis of DSS-induced colitis in mice.

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Authors and Affiliations

  1. Department of Surgery, Penn State College of Medicine, Hershey, Pennsylvania, 17033, USA

    Leo R. Fitzpatrick PhD, Vineeta Khare PhD, Jeffrey S. Small BA & Walter A. Koltun MD

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  1. Leo R. Fitzpatrick PhD
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  2. Vineeta Khare PhD
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  3. Jeffrey S. Small BA
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  4. Walter A. Koltun MD
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Correspondence to Leo R. Fitzpatrick PhD.

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Fitzpatrick, L.R., Khare, V., Small, J.S. et al. Dextran Sulfate Sodium-Induced Colitis Is Associated with Enhanced Low Molecular Mass Polypeptide 2 (LMP2) Expression and Is Attenuated in LMP2 Knockout Mice. Dig Dis Sci 51, 1269–1276 (2006). https://doi.org/10.1007/s10620-006-8047-2

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  • DOI: https://doi.org/10.1007/s10620-006-8047-2

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