Abstract
Ulcerative colitis (UC) is a complex, multifactorial disorder which can be aggravated by oxidative stress. Dextran sulfate sodium (DSS)-induced UC in inbred mice is the most commonly used animal-model. However, these populations lack genetic variability therefore this study aimed to establish a DSS-induced UC model in outbred ICR mice and to assess its association with the oxidant-antioxidant system. Male ICR mice were administered a 40 kDa-DSS solution (6 g/kg/day; 0.25 mL 21% DSS 4 times per day) intragastrically for 4 and 7 consecutive days (n = 5). Disease activity index (DAI) was determined daily from body weight and stool characteristics. At the end of the study, colons were collected to examine histology, myeloperoxidase activity, and expression of superoxide dismutase 1 and 2 (Sod1 and Sod2) and catalase (Cat). DSS-treated mice demonstrated daily increases in DAI score with significantly shortened colons, an indirect marker of inflammation in UC. Histological examination of DSS-treated colons revealed goblet cell loss, crypt damage, and epithelial erosion, followed by infiltration of mast cells to the mucosa with mucin depletion. Myeloperoxidase activity was elevated in the DSS-treated mice while Sod1, Sod2, and Cat were suppressed compared to controls. A DSS-induced UC model was established in ICR mice with associated oxidative stress triggering. This outbred-mouse model could prove useful for investigating the underlying pathophysiological mechanisms of UC in terms of oxidant-antioxidant balance.
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Abbreviations
- CAT:
-
catalase
- DSS:
-
dextran sulfate sodium
- DAI:
-
disease activity index
- IBD:
-
inflammatory bowel disease
- MPO:
-
myeloperoxidas
- ROS:
-
reactive oxygen species
- SOD:
-
superoxide dismutase
- UC:
-
ulcerative colitis
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Acknowledgements
Nitima Tatiya-aphiradee acknowledges the Royal Golden Jubilee Ph.D. program, Thailand Research Fund for the scholarship (PhD0008/2559). The authors thank Dr. Glenn Borlace, Faculty of Pharmaceutical Sciences, Khon Kaen University for English language assistance.
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Tatiya-aphiradee, N., Chatuphonprasert, W. & Jarukamjorn, K. Oxidative stress exacerbates dextran sulfate sodium-induced ulcerative colitis in ICR mice. Biologia 75, 2063–2071 (2020). https://doi.org/10.2478/s11756-020-00524-9
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DOI: https://doi.org/10.2478/s11756-020-00524-9