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Modulation of heparan sulfate biosynthesis by sodium butyrate in recombinant CHO cells

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Abstract

Sodium butyrate, a histone deacetylase inhibitor, has been used to improve transgene expression in Chinese hamster ovary (CHO) cells. The current study explores the impact of butyrate treatment on heparan sulfate (HS) biosynthesis and structural composition in a recombinant CHO-S cell line expressing enzymes in the heparin (HP)/(HS) biosynthetic pathway (Dual-10 stably expressing NDST2 and HS3st1). Flow cytometric analysis showed that antithrombin binding was increased in Dual-10 cells and basic fibroblast growth factor binding was decreased in response to sodium butyrate treatment. The results were in agreement with the AMAC-LCMS (2-aminoacridine-tagged HS/HP analysis by liquid chromatography mass spectrometry) data that showed that there was an increase in heparan sulfate tri-sulfated disaccharides and a decrease in N-sulfated disaccharides in the butyrate-treated cells. However, we could not detect any changes in the chondroitin sulfate pathway in Dual-10 cells treated with butyrate. The current study is the first to report the effect of butyrate on glycosaminoglycan profiles.

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Acknowledgments

This work was supported in part by a grant from the National Institutes of Health (R01GM090127). Payel Datta was supported in part by a fellowship from Rensselaer Polytechnic Institute.

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Authors and Affiliations

  1. Department of Biology and Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute, 110 8th Avenue, Troy, NY, 12180, USA

    Payel Datta & Robert J. Linhardt

  2. SUNY College of Nanoscale Science and Engineering, 257 Fuller Road, Albany, NY, 12203, USA

    Susan T. Sharfstein

  3. Department of Chemistry and Chemical Biology and Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute, 110 8th Avenue, Troy, NY, 12180, USA

    Bo Yang & Robert J. Linhardt

  4. Department of Chemical and Biological Engineering and Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute, 110 8th Avenue, Troy, NY, 12180, USA

    Robert J. Linhardt

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  1. Payel Datta
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  2. Bo Yang
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  4. Susan T. Sharfstein
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Correspondence to Susan T. Sharfstein.

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Datta, P., Yang, B., Linhardt, R.J. et al. Modulation of heparan sulfate biosynthesis by sodium butyrate in recombinant CHO cells. Cytotechnology 67, 223–235 (2015). https://doi.org/10.1007/s10616-013-9677-9

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