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Influence of different ammonium, lactate and glutamine concentrations on CCO cell growth

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Abstract

In this study the effects of ammonium and lactate on a culture of channel catfish ovary (CCO) cells were examined. We also made investigation on the influence of glutamine, since our previous research revealed that this amino acid stimulated CCO cell growth more than glucose in a concentration-dependent manner. The effect of ammonium in cell culture included the considerable decrease in cell growth rate with eventual growth arrest as well as the retardation of glucose consumption. At ammonium concentrations above 2.5 mM, the cells displayed specific morphological changes. The effect of lactate was different to that of ammonium since the cell growth rate was progressively decreasing with the increase of lactate concentration, whereas the glucose consumption rate remained almost unchanged. Besides that, it was found that lactate was steadily eliminated from the culture medium when its initial concentration was relatively high. The influence of glutamine on CCO cell propagation showed that nutrient requirements of this cell line were mainly dependent on glutamine rather than glucose. The increase in glutamine concentration led to the increase in cell growth rate and consequent ammonia accumulation while the glucose utilization and lactate production were reduced. Without glutamine in culture medium cell growth was arrested. However, the lack of glucose reversed the stimulating effect of glutamine by decreasing cell growth rate and affecting amino acid utilization.

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Acknowledgments

This work was supported by the Ministry of Science, Education and Sports of the Republic of Croatia (Grant No. 058-0582184-2141). The authors are greatful to Prof. K. Fumic Ph.D. and K. Bilic M.Sc. of Laboratory for Diagnosis of Metabolic Diseases, Clinical Hospital Centre Zagreb, for amino acid analysis.

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  1. Laboratory of Cell Culture Technology and Biotransformation, Faculty of Food Technology and Biotechnology, University of Zagreb, 6 Pierotti St., 10000, Zagreb, Croatia

    Igor Slivac, Višnja Blajić, Kristina Radošević, Zlatko Kniewald & Višnja Gaurina Srček

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  1. Igor Slivac
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  2. Višnja Blajić
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  3. Kristina Radošević
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  4. Zlatko Kniewald
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  5. Višnja Gaurina Srček
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Correspondence to Višnja Gaurina Srček.

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Slivac, I., Blajić, V., Radošević, K. et al. Influence of different ammonium, lactate and glutamine concentrations on CCO cell growth. Cytotechnology 62, 585–594 (2010). https://doi.org/10.1007/s10616-010-9312-y

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