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Growth characteristics of channel catfish ovary cells—influence of glucose and glutamine

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Abstract

The growth characteristics and influence of glucose and glutamine on the growth and maintenance of channel catfish ovary (CCO) cells were investigated. Besides glutamine, amino acids threonine, arginine, methionine and serine were found to be essential for CCO cell growth. In the glucose-free medium, glutamine is utilized as energy source and no cell growth limitation was observed. However, the lack of glutamine in culture medium did not stimulate CCO cells to efficient glucose consumption. When both glucose and glutamine were deficient, cell growth was also observed suggesting no rigorous nutritional requirements. Obtained results are useful for further understanding of culture processes using CCO cells.

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Acknowledgement

This work was supported by the Ministry of Science, Education and Sports of the Republic of Croatia (Grant No. 058-0582184-2141).

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Authors and Affiliations

  1. Laboratory of Cell Culture Technology, Application and Biotransformation, Faculty of Food Technology and Biotechnology, University of Zagreb, 6 Pierotti St., 10000, Zagreb, Croatia

    Igor Slivac, Višnja Gaurina Srček, Kristina Radošević, Ivana Porobić & Zlatko Kniewald

  2. Laboratory for Diagnosis of Metabolic Diseases, Clinical Hospital Centre Zagreb, 12 Kispatic St., 10000, Zagreb, Croatia

    Karmen Bilić & Ksenija Fumić

Authors
  1. Igor Slivac
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  2. Višnja Gaurina Srček
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  3. Kristina Radošević
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  4. Ivana Porobić
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  5. Karmen Bilić
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  6. Ksenija Fumić
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  7. Zlatko Kniewald
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Correspondence to Zlatko Kniewald.

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Slivac, I., Gaurina Srček, V., Radošević, K. et al. Growth characteristics of channel catfish ovary cells—influence of glucose and glutamine. Cytotechnology 57, 273–278 (2008). https://doi.org/10.1007/s10616-008-9171-y

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  • DOI: https://doi.org/10.1007/s10616-008-9171-y

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